Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF181 and GFF182 are separated by 43 nucleotides GFF182 and GFF183 are separated by 173 nucleotides
GFF181 - FIG01046907: hypothetical protein, at 172,900 to 173,613
GFF181
GFF182 - DNA polymerase II (EC 2.7.7.7), at 173,657 to 176,008
GFF182
GFF183 - RNA polymerase associated protein RapA (EC 3.6.1.-), at 176,182 to 179,088
GFF183
Position (kb) 173
174
175
176
177 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 172.668 kb on + strand at 172.721 kb on - strand at 173.073 kb on + strand, within GFF181 at 173.077 kb on + strand, within GFF181 at 173.288 kb on - strand, within GFF181 at 173.506 kb on - strand, within GFF181 at 173.554 kb on + strand at 173.623 kb on + strand at 173.623 kb on + strand at 173.924 kb on - strand, within GFF182 at 174.016 kb on + strand, within GFF182 at 174.017 kb on - strand, within GFF182 at 174.077 kb on - strand, within GFF182 at 174.077 kb on - strand, within GFF182 at 174.152 kb on + strand, within GFF182 at 174.193 kb on + strand, within GFF182 at 174.193 kb on + strand, within GFF182 at 174.194 kb on - strand, within GFF182 at 174.194 kb on - strand, within GFF182 at 174.194 kb on - strand, within GFF182 at 174.245 kb on + strand, within GFF182 at 174.246 kb on - strand, within GFF182 at 174.246 kb on - strand, within GFF182 at 174.331 kb on - strand, within GFF182 at 174.349 kb on + strand, within GFF182 at 174.380 kb on - strand, within GFF182 at 174.786 kb on - strand, within GFF182 at 174.786 kb on - strand, within GFF182 at 174.812 kb on + strand, within GFF182 at 174.864 kb on - strand, within GFF182 at 174.868 kb on - strand, within GFF182 at 175.006 kb on + strand, within GFF182 at 175.141 kb on + strand, within GFF182 at 175.155 kb on - strand, within GFF182 at 175.284 kb on - strand, within GFF182 at 175.284 kb on - strand, within GFF182 at 175.614 kb on - strand, within GFF182 at 175.614 kb on - strand, within GFF182 at 175.614 kb on - strand, within GFF182 at 175.614 kb on - strand, within GFF182 at 175.649 kb on + strand, within GFF182 at 175.650 kb on - strand, within GFF182 at 175.731 kb on + strand, within GFF182 at 175.732 kb on - strand, within GFF182 at 175.845 kb on - strand at 175.845 kb on - strand at 175.902 kb on - strand at 175.911 kb on - strand at 175.927 kb on - strand at 175.976 kb on - strand at 175.980 kb on + strand at 176.085 kb on + strand at 176.085 kb on + strand at 176.086 kb on - strand at 176.160 kb on - strand at 176.167 kb on + strand at 176.190 kb on + strand at 176.277 kb on - strand at 176.364 kb on + strand at 176.402 kb on + strand at 176.429 kb on - strand at 176.658 kb on + strand, within GFF183 at 176.676 kb on - strand, within GFF183 at 176.727 kb on + strand, within GFF183
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 172,668 + -0.6 172,721 - -0.5 173,073 + GFF181 0.24 -2.6 173,077 + GFF181 0.25 -0.3 173,288 - GFF181 0.54 -0.9 173,506 - GFF181 0.85 +0.1 173,554 + -0.1 173,623 + +0.5 173,623 + -0.4 173,924 - GFF182 0.11 +0.2 174,016 + GFF182 0.15 +0.7 174,017 - GFF182 0.15 +0.4 174,077 - GFF182 0.18 -1.7 174,077 - GFF182 0.18 -0.4 174,152 + GFF182 0.21 -3.6 174,193 + GFF182 0.23 -0.6 174,193 + GFF182 0.23 -2.0 174,194 - GFF182 0.23 -0.0 174,194 - GFF182 0.23 +0.8 174,194 - GFF182 0.23 -0.6 174,245 + GFF182 0.25 +0.2 174,246 - GFF182 0.25 -0.6 174,246 - GFF182 0.25 +0.4 174,331 - GFF182 0.29 +0.8 174,349 + GFF182 0.29 +1.2 174,380 - GFF182 0.31 +1.2 174,786 - GFF182 0.48 -0.0 174,786 - GFF182 0.48 -1.2 174,812 + GFF182 0.49 +0.0 174,864 - GFF182 0.51 +0.4 174,868 - GFF182 0.51 +1.6 175,006 + GFF182 0.57 +1.1 175,141 + GFF182 0.63 -0.1 175,155 - GFF182 0.64 +0.6 175,284 - GFF182 0.69 -0.4 175,284 - GFF182 0.69 +0.2 175,614 - GFF182 0.83 +1.2 175,614 - GFF182 0.83 +1.3 175,614 - GFF182 0.83 -0.2 175,614 - GFF182 0.83 +1.0 175,649 + GFF182 0.85 -3.8 175,650 - GFF182 0.85 +0.1 175,731 + GFF182 0.88 +0.5 175,732 - GFF182 0.88 +1.0 175,845 - +0.6 175,845 - -0.0 175,902 - +1.3 175,911 - +0.4 175,927 - -0.6 175,976 - +1.0 175,980 + +0.2 176,085 + +0.5 176,085 + +1.0 176,086 - -2.0 176,160 - +0.2 176,167 + -0.8 176,190 + -3.3 176,277 - -0.3 176,364 + -1.6 176,402 + -0.6 176,429 - +0.2 176,658 + GFF183 0.16 -0.6 176,676 - GFF183 0.17 -2.8 176,727 + GFF183 0.19 -0.2
Or see this region's nucleotide sequence