Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1478

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1477 and GFF1478 are separated by 95 nucleotidesGFF1478 and GFF1479 are separated by 146 nucleotidesGFF1479 and GFF1480 are separated by 409 nucleotides GFF1477 - Fumarate hydratase class I, anaerobic (EC 4.2.1.2), at 145,496 to 147,142 GFF1477 GFF1478 - C4-dicarboxylate transporter DcuB, at 147,238 to 148,578 GFF1478 GFF1479 - hypothetical protein, at 148,725 to 148,898 GFF1479 GFF1480 - Fumarate respiration transcriptional regulator DcuR, at 149,308 to 150,027 GFF1480 Position (kb) 147 148 149Strain fitness (log2 ratio) -2 -1 0 1 2at 146.243 kb on + strand, within GFF1477at 146.386 kb on - strand, within GFF1477at 146.464 kb on - strand, within GFF1477at 146.557 kb on - strand, within GFF1477at 146.557 kb on - strand, within GFF1477at 146.562 kb on + strand, within GFF1477at 146.610 kb on + strand, within GFF1477at 146.679 kb on + strand, within GFF1477at 146.679 kb on + strand, within GFF1477at 146.683 kb on + strand, within GFF1477at 146.684 kb on - strand, within GFF1477at 146.727 kb on + strand, within GFF1477at 146.727 kb on + strand, within GFF1477at 146.728 kb on - strand, within GFF1477at 146.728 kb on - strand, within GFF1477at 146.731 kb on + strand, within GFF1477at 146.732 kb on - strand, within GFF1477at 146.889 kb on + strand, within GFF1477at 146.889 kb on + strand, within GFF1477at 147.028 kb on + strandat 147.079 kb on - strandat 147.081 kb on + strandat 147.158 kb on + strandat 147.408 kb on + strand, within GFF1478at 147.410 kb on + strand, within GFF1478at 147.410 kb on + strand, within GFF1478at 147.410 kb on + strand, within GFF1478at 147.411 kb on - strand, within GFF1478at 147.411 kb on - strand, within GFF1478at 147.411 kb on - strand, within GFF1478at 147.414 kb on - strand, within GFF1478at 147.414 kb on - strand, within GFF1478at 147.805 kb on + strand, within GFF1478at 147.806 kb on - strand, within GFF1478at 147.926 kb on + strand, within GFF1478at 148.002 kb on + strand, within GFF1478at 148.207 kb on - strand, within GFF1478at 148.309 kb on - strand, within GFF1478at 148.309 kb on - strand, within GFF1478at 148.309 kb on - strand, within GFF1478at 148.316 kb on + strand, within GFF1478at 148.317 kb on - strand, within GFF1478at 148.427 kb on - strand, within GFF1478at 148.544 kb on - strandat 148.937 kb on + strandat 149.318 kb on + strandat 149.527 kb on + strand, within GFF1480at 149.527 kb on + strand, within GFF1480

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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146,243 + GFF1477 0.45 -2.7
146,386 - GFF1477 0.54 -0.9
146,464 - GFF1477 0.59 +1.3
146,557 - GFF1477 0.64 -2.0
146,557 - GFF1477 0.64 +0.5
146,562 + GFF1477 0.65 -0.2
146,610 + GFF1477 0.68 +0.3
146,679 + GFF1477 0.72 +0.2
146,679 + GFF1477 0.72 -0.9
146,683 + GFF1477 0.72 +0.2
146,684 - GFF1477 0.72 +0.5
146,727 + GFF1477 0.75 -1.2
146,727 + GFF1477 0.75 +0.5
146,728 - GFF1477 0.75 +0.6
146,728 - GFF1477 0.75 +0.6
146,731 + GFF1477 0.75 -0.9
146,732 - GFF1477 0.75 +0.4
146,889 + GFF1477 0.85 -0.2
146,889 + GFF1477 0.85 +0.6
147,028 + +1.0
147,079 - -0.3
147,081 + +1.2
147,158 + +0.2
147,408 + GFF1478 0.13 +0.3
147,410 + GFF1478 0.13 -0.5
147,410 + GFF1478 0.13 +0.1
147,410 + GFF1478 0.13 -0.8
147,411 - GFF1478 0.13 -0.4
147,411 - GFF1478 0.13 +0.8
147,411 - GFF1478 0.13 -0.3
147,414 - GFF1478 0.13 +0.7
147,414 - GFF1478 0.13 -0.1
147,805 + GFF1478 0.42 +1.6
147,806 - GFF1478 0.42 -0.8
147,926 + GFF1478 0.51 -0.8
148,002 + GFF1478 0.57 -0.2
148,207 - GFF1478 0.72 +0.8
148,309 - GFF1478 0.80 +2.5
148,309 - GFF1478 0.80 +1.2
148,309 - GFF1478 0.80 -0.5
148,316 + GFF1478 0.80 -1.5
148,317 - GFF1478 0.80 +0.8
148,427 - GFF1478 0.89 +1.5
148,544 - +0.6
148,937 + +2.3
149,318 + +0.9
149,527 + GFF1480 0.30 -2.3
149,527 + GFF1480 0.30 +2.6

Or see this region's nucleotide sequence