Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1385

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1384 and GFF1385 are separated by 96 nucleotidesGFF1385 and GFF1386 overlap by 41 nucleotidesGFF1386 and GFF1387 are separated by 51 nucleotidesGFF1387 and GFF1388 overlap by 1 nucleotides GFF1384 - Phage tail length tape-measure protein, at 30,662 to 33,016 GFF1384 GFF1385 - hypothetical protein, at 33,113 to 33,241 GFF1385 GFF1386 - FIG00639134: hypothetical protein, at 33,201 to 33,518 GFF1386 GFF1387 - Putative phage tail core protein, at 33,570 to 34,094 GFF1387 GFF1388 - Phage tail sheath monomer, at 34,094 to 35,521 GFF1388 Position (kb) 33 34Strain fitness (log2 ratio) -1 0 1at 32.427 kb on + strand, within GFF1384at 32.428 kb on - strand, within GFF1384at 32.428 kb on - strand, within GFF1384at 32.434 kb on + strand, within GFF1384at 32.559 kb on + strand, within GFF1384at 33.001 kb on + strandat 33.019 kb on + strandat 33.173 kb on - strand, within GFF1385at 33.314 kb on - strand, within GFF1386at 33.447 kb on + strand, within GFF1386at 33.448 kb on - strand, within GFF1386at 33.485 kb on + strand, within GFF1386at 33.522 kb on - strandat 33.582 kb on - strandat 33.813 kb on - strand, within GFF1387at 33.872 kb on + strand, within GFF1387at 33.984 kb on - strand, within GFF1387at 34.008 kb on - strand, within GFF1387

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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32,427 + GFF1384 0.75 +1.1
32,428 - GFF1384 0.75 +0.2
32,428 - GFF1384 0.75 +0.1
32,434 + GFF1384 0.75 -0.6
32,559 + GFF1384 0.81 +1.0
33,001 + +0.4
33,019 + +0.3
33,173 - GFF1385 0.47 -0.9
33,314 - GFF1386 0.36 -0.7
33,447 + GFF1386 0.77 +0.9
33,448 - GFF1386 0.78 +0.6
33,485 + GFF1386 0.89 +1.0
33,522 - +0.7
33,582 - -0.1
33,813 - GFF1387 0.46 -0.7
33,872 + GFF1387 0.58 -0.0
33,984 - GFF1387 0.79 +0.6
34,008 - GFF1387 0.83 +1.0

Or see this region's nucleotide sequence