Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1381 and GFF1382 overlap by 13 nucleotides GFF1382 and GFF1383 overlap by 1 nucleotides GFF1383 and GFF1384 overlap by 1 nucleotides
GFF1381 - Gene D protein, at 28,469 to 29,512
GFF1381
GFF1382 - Putative inner membrane protein, at 29,500 to 29,709
GFF1382
GFF1383 - FIG00639790: hypothetical protein, at 29,709 to 30,662
GFF1383
GFF1384 - Phage tail length tape-measure protein, at 30,662 to 33,016
GFF1384
Position (kb)
29
30
31 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 28.864 kb on + strand, within GFF1381 at 28.865 kb on - strand, within GFF1381 at 28.879 kb on - strand, within GFF1381 at 28.980 kb on + strand, within GFF1381 at 28.981 kb on - strand, within GFF1381 at 28.981 kb on - strand, within GFF1381 at 29.126 kb on - strand, within GFF1381 at 29.241 kb on + strand, within GFF1381 at 29.381 kb on - strand, within GFF1381 at 29.384 kb on - strand, within GFF1381 at 29.413 kb on - strand at 29.413 kb on - strand at 29.427 kb on + strand at 29.441 kb on - strand at 29.501 kb on + strand at 29.547 kb on + strand, within GFF1382 at 29.703 kb on - strand at 29.724 kb on - strand at 30.162 kb on - strand, within GFF1383 at 30.584 kb on - strand at 30.600 kb on - strand at 30.663 kb on + strand at 30.664 kb on - strand at 30.842 kb on - strand at 30.842 kb on - strand at 30.843 kb on + strand at 30.843 kb on + strand at 31.162 kb on + strand, within GFF1384 at 31.217 kb on + strand, within GFF1384 at 31.363 kb on - strand, within GFF1384 at 31.367 kb on + strand, within GFF1384
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 28,864 + GFF1381 0.38 -2.5 28,865 - GFF1381 0.38 -2.8 28,879 - GFF1381 0.39 -0.3 28,980 + GFF1381 0.49 -0.6 28,981 - GFF1381 0.49 -0.3 28,981 - GFF1381 0.49 +0.6 29,126 - GFF1381 0.63 -0.2 29,241 + GFF1381 0.74 +2.1 29,381 - GFF1381 0.87 -0.2 29,384 - GFF1381 0.88 +0.0 29,413 - -0.9 29,413 - +0.2 29,427 + +1.2 29,441 - +1.8 29,501 + +1.0 29,547 + GFF1382 0.22 -1.1 29,703 - -2.0 29,724 - +1.1 30,162 - GFF1383 0.47 +0.8 30,584 - +0.4 30,600 - +0.4 30,663 + +0.6 30,664 - -0.6 30,842 - +0.7 30,842 - +0.7 30,843 + +0.8 30,843 + -0.5 31,162 + GFF1384 0.21 +0.6 31,217 + GFF1384 0.24 +0.1 31,363 - GFF1384 0.30 +0.6 31,367 + GFF1384 0.30 -0.1
Or see this region's nucleotide sequence