Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1176

Experiment: 6H2_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1175 and GFF1176 are separated by 19 nucleotidesGFF1176 and GFF1177 are separated by 8 nucleotidesGFF1177 and GFF1178 overlap by 32 nucleotidesGFF1178 and GFF1179 are separated by 113 nucleotides GFF1175 - IncF plasmid conjugative transfer protein TraN, at 20,907 to 22,733 GFF1175 GFF1176 - IncF plasmid conjugative transfer protein TrbE, at 22,753 to 22,959 GFF1176 GFF1177 - IncF plasmid conjugative transfer pilus assembly protein TraF, at 22,968 to 23,720 GFF1177 GFF1178 - hypothetical protein, at 23,689 to 23,820 GFF1178 GFF1179 - IncF plasmid conjugative transfer protein TraQ, at 23,934 to 24,227 GFF1179 Position (kb) 22 23Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 21.773 kb on - strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.945 kb on + strand, within GFF1175at 21.946 kb on - strand, within GFF1175at 21.946 kb on - strand, within GFF1175at 21.946 kb on - strand, within GFF1175at 21.946 kb on - strand, within GFF1175at 21.984 kb on + strand, within GFF1175at 21.984 kb on + strand, within GFF1175at 21.985 kb on - strand, within GFF1175at 22.062 kb on + strand, within GFF1175at 22.062 kb on + strand, within GFF1175at 22.072 kb on - strand, within GFF1175at 22.220 kb on + strand, within GFF1175at 22.470 kb on + strand, within GFF1175at 22.602 kb on - strandat 22.731 kb on + strandat 22.732 kb on - strandat 22.751 kb on + strandat 22.751 kb on + strandat 22.752 kb on - strandat 22.786 kb on - strand, within GFF1176at 22.786 kb on - strand, within GFF1176at 22.826 kb on + strand, within GFF1176at 22.870 kb on - strand, within GFF1176at 22.870 kb on - strand, within GFF1176at 22.879 kb on + strand, within GFF1176at 22.879 kb on + strand, within GFF1176at 22.880 kb on - strand, within GFF1176at 22.880 kb on - strand, within GFF1176at 22.944 kb on + strandat 22.967 kb on + strandat 22.967 kb on + strandat 22.968 kb on - strandat 22.968 kb on - strandat 23.005 kb on + strandat 23.055 kb on + strand, within GFF1177at 23.056 kb on - strand, within GFF1177at 23.097 kb on - strand, within GFF1177at 23.130 kb on - strand, within GFF1177at 23.130 kb on - strand, within GFF1177at 23.217 kb on + strand, within GFF1177at 23.307 kb on + strand, within GFF1177at 23.307 kb on + strand, within GFF1177at 23.308 kb on - strand, within GFF1177at 23.308 kb on - strand, within GFF1177at 23.319 kb on + strand, within GFF1177at 23.329 kb on - strand, within GFF1177at 23.341 kb on + strand, within GFF1177at 23.342 kb on - strand, within GFF1177at 23.406 kb on + strand, within GFF1177at 23.407 kb on - strand, within GFF1177at 23.412 kb on - strand, within GFF1177at 23.425 kb on - strand, within GFF1177at 23.468 kb on + strand, within GFF1177at 23.487 kb on + strand, within GFF1177at 23.487 kb on + strand, within GFF1177at 23.487 kb on + strand, within GFF1177at 23.612 kb on - strand, within GFF1177at 23.655 kb on - strandat 23.739 kb on + strand, within GFF1178at 23.739 kb on + strand, within GFF1178at 23.905 kb on - strandat 23.907 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay
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21,773 - GFF1175 0.47 -0.7
21,945 + GFF1175 0.57 +0.1
21,945 + GFF1175 0.57 +1.5
21,945 + GFF1175 0.57 +0.8
21,945 + GFF1175 0.57 -0.0
21,945 + GFF1175 0.57 +0.1
21,946 - GFF1175 0.57 +0.3
21,946 - GFF1175 0.57 -1.3
21,946 - GFF1175 0.57 -0.2
21,946 - GFF1175 0.57 -3.7
21,984 + GFF1175 0.59 -0.2
21,984 + GFF1175 0.59 -0.3
21,985 - GFF1175 0.59 -3.8
22,062 + GFF1175 0.63 -0.7
22,062 + GFF1175 0.63 +1.2
22,072 - GFF1175 0.64 +0.8
22,220 + GFF1175 0.72 -0.0
22,470 + GFF1175 0.86 +1.3
22,602 - -1.4
22,731 + -0.2
22,732 - +0.5
22,751 + -2.1
22,751 + -3.0
22,752 - -1.3
22,786 - GFF1176 0.16 +0.1
22,786 - GFF1176 0.16 -3.1
22,826 + GFF1176 0.35 -0.7
22,870 - GFF1176 0.57 -4.1
22,870 - GFF1176 0.57 -1.3
22,879 + GFF1176 0.61 +0.6
22,879 + GFF1176 0.61 +0.8
22,880 - GFF1176 0.61 -0.5
22,880 - GFF1176 0.61 +0.7
22,944 + +0.2
22,967 + +0.2
22,967 + +1.3
22,968 - +0.5
22,968 - -0.3
23,005 + +1.8
23,055 + GFF1177 0.12 +0.1
23,056 - GFF1177 0.12 +1.1
23,097 - GFF1177 0.17 -1.4
23,130 - GFF1177 0.22 +1.1
23,130 - GFF1177 0.22 -0.1
23,217 + GFF1177 0.33 +0.2
23,307 + GFF1177 0.45 -0.3
23,307 + GFF1177 0.45 +1.1
23,308 - GFF1177 0.45 +0.6
23,308 - GFF1177 0.45 +0.7
23,319 + GFF1177 0.47 +0.4
23,329 - GFF1177 0.48 +1.1
23,341 + GFF1177 0.50 +0.7
23,342 - GFF1177 0.50 -4.7
23,406 + GFF1177 0.58 -2.8
23,407 - GFF1177 0.58 +0.2
23,412 - GFF1177 0.59 -0.7
23,425 - GFF1177 0.61 +0.4
23,468 + GFF1177 0.66 +0.8
23,487 + GFF1177 0.69 -0.1
23,487 + GFF1177 0.69 +0.6
23,487 + GFF1177 0.69 +1.3
23,612 - GFF1177 0.86 +0.1
23,655 - -2.6
23,739 + GFF1178 0.38 +0.6
23,739 + GFF1178 0.38 -0.8
23,905 - +1.6
23,907 - +0.4

Or see this region's nucleotide sequence