Experiment: 6H2_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1131 and GFF1132 are separated by 21 nucleotides GFF1132 and GFF1133 are separated by 1 nucleotides GFF1133 and GFF1134 are separated by 95 nucleotides
GFF1131 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 320,948 to 321,868
GFF1131
GFF1132 - Transcriptional regulatory protein CitB, DpiA, at 321,890 to 322,576
GFF1132
GFF1133 - Sensor kinase CitA, DpiB (EC 2.7.3.-), at 322,578 to 324,236
GFF1133
GFF1134 - Oxaloacetate decarboxylase beta chain (EC 4.1.1.3), at 324,332 to 324,595
GFF1134
Position (kb) 322
323
324
325 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 321.625 kb on + strand, within GFF1131 at 321.870 kb on + strand at 321.871 kb on - strand at 321.907 kb on - strand at 321.937 kb on - strand at 322.039 kb on + strand, within GFF1132 at 322.039 kb on + strand, within GFF1132 at 322.040 kb on - strand, within GFF1132 at 322.043 kb on + strand, within GFF1132 at 322.044 kb on - strand, within GFF1132 at 322.064 kb on + strand, within GFF1132 at 322.065 kb on - strand, within GFF1132 at 322.150 kb on + strand, within GFF1132 at 322.248 kb on + strand, within GFF1132 at 322.313 kb on - strand, within GFF1132 at 322.367 kb on - strand, within GFF1132 at 322.421 kb on + strand, within GFF1132 at 322.565 kb on - strand at 322.603 kb on + strand at 322.603 kb on + strand at 322.604 kb on - strand at 322.604 kb on - strand at 322.647 kb on - strand at 322.672 kb on + strand at 322.673 kb on - strand at 322.805 kb on + strand, within GFF1133 at 322.871 kb on - strand, within GFF1133 at 323.039 kb on + strand, within GFF1133 at 323.226 kb on - strand, within GFF1133 at 323.253 kb on - strand, within GFF1133 at 323.542 kb on - strand, within GFF1133 at 323.589 kb on - strand, within GFF1133 at 323.589 kb on - strand, within GFF1133 at 323.617 kb on + strand, within GFF1133 at 323.702 kb on + strand, within GFF1133 at 323.778 kb on + strand, within GFF1133 at 323.803 kb on + strand, within GFF1133 at 323.816 kb on + strand, within GFF1133 at 323.819 kb on - strand, within GFF1133 at 323.851 kb on + strand, within GFF1133 at 323.852 kb on - strand, within GFF1133 at 323.917 kb on + strand, within GFF1133 at 324.033 kb on - strand, within GFF1133 at 324.089 kb on - strand at 324.198 kb on - strand at 324.204 kb on - strand at 324.331 kb on - strand at 324.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6H2_phage 10 MOI; agar plate assay remove 321,625 + GFF1131 0.74 +1.4 321,870 + +0.2 321,871 - -0.4 321,907 - -1.9 321,937 - -1.6 322,039 + GFF1132 0.22 +1.2 322,039 + GFF1132 0.22 +1.5 322,040 - GFF1132 0.22 +0.0 322,043 + GFF1132 0.22 +0.6 322,044 - GFF1132 0.22 +0.2 322,064 + GFF1132 0.25 -0.5 322,065 - GFF1132 0.25 -0.2 322,150 + GFF1132 0.38 -0.4 322,248 + GFF1132 0.52 -3.6 322,313 - GFF1132 0.62 -1.8 322,367 - GFF1132 0.69 +0.7 322,421 + GFF1132 0.77 +0.2 322,565 - +0.3 322,603 + +0.2 322,603 + +0.3 322,604 - +1.0 322,604 - +0.0 322,647 - +0.7 322,672 + +0.7 322,673 - +0.7 322,805 + GFF1133 0.14 -0.5 322,871 - GFF1133 0.18 +0.1 323,039 + GFF1133 0.28 -3.1 323,226 - GFF1133 0.39 +0.0 323,253 - GFF1133 0.41 -0.0 323,542 - GFF1133 0.58 +0.2 323,589 - GFF1133 0.61 +2.2 323,589 - GFF1133 0.61 -2.4 323,617 + GFF1133 0.63 -2.0 323,702 + GFF1133 0.68 -0.1 323,778 + GFF1133 0.72 +2.5 323,803 + GFF1133 0.74 +0.4 323,816 + GFF1133 0.75 +1.5 323,819 - GFF1133 0.75 -2.2 323,851 + GFF1133 0.77 +0.3 323,852 - GFF1133 0.77 -0.6 323,917 + GFF1133 0.81 -0.1 324,033 - GFF1133 0.88 -2.1 324,089 - -1.3 324,198 - +0.5 324,204 - -0.8 324,331 - -0.6 324,334 - +0.0
Or see this region's nucleotide sequence