Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF92

Experiment: Ffm_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF91 and GFF92 are separated by 125 nucleotidesGFF92 and GFF93 are separated by 94 nucleotidesGFF93 and GFF94 are separated by 6 nucleotides GFF91 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 75,813 to 76,604 GFF91 GFF92 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 76,730 to 77,584 GFF92 GFF93 - Aspartate 1-decarboxylase (EC 4.1.1.11), at 77,679 to 78,059 GFF93 GFF94 - Polysaccharide deacetylase, at 78,066 to 79,295 GFF94 Position (kb) 76 77 78Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 75.773 kb on - strandat 75.773 kb on - strandat 75.796 kb on + strandat 75.907 kb on + strand, within GFF91at 75.923 kb on + strand, within GFF91at 75.924 kb on - strand, within GFF91at 76.005 kb on - strand, within GFF91at 76.051 kb on + strand, within GFF91at 76.074 kb on - strand, within GFF91at 76.248 kb on - strand, within GFF91at 76.318 kb on + strand, within GFF91at 76.484 kb on + strand, within GFF91at 76.548 kb on + strandat 76.548 kb on + strandat 76.548 kb on + strandat 76.549 kb on - strandat 76.549 kb on - strandat 76.550 kb on + strandat 76.551 kb on - strandat 76.551 kb on - strandat 76.551 kb on - strandat 76.679 kb on + strandat 76.734 kb on + strandat 76.769 kb on - strandat 76.994 kb on + strand, within GFF92at 77.051 kb on + strand, within GFF92at 77.051 kb on + strand, within GFF92at 77.052 kb on - strand, within GFF92at 77.297 kb on - strand, within GFF92at 77.304 kb on - strand, within GFF92at 77.342 kb on + strand, within GFF92at 77.356 kb on + strand, within GFF92at 77.357 kb on - strand, within GFF92at 77.405 kb on - strand, within GFF92at 77.649 kb on - strandat 77.789 kb on + strand, within GFF93at 77.813 kb on + strand, within GFF93at 77.851 kb on - strand, within GFF93at 78.060 kb on + strandat 78.131 kb on - strandat 78.270 kb on + strand, within GFF94at 78.335 kb on - strand, within GFF94at 78.348 kb on + strand, within GFF94

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Per-strain Table

Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay
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75,773 - -0.4
75,773 - +2.2
75,796 + +0.9
75,907 + GFF91 0.12 -2.0
75,923 + GFF91 0.14 +0.7
75,924 - GFF91 0.14 +0.6
76,005 - GFF91 0.24 -0.3
76,051 + GFF91 0.30 -0.4
76,074 - GFF91 0.33 +0.8
76,248 - GFF91 0.55 -0.4
76,318 + GFF91 0.64 -2.1
76,484 + GFF91 0.85 -0.1
76,548 + -0.7
76,548 + +0.5
76,548 + -1.6
76,549 - +0.3
76,549 - +1.9
76,550 + +1.5
76,551 - -0.1
76,551 - +0.1
76,551 - -0.7
76,679 + +0.1
76,734 + +1.1
76,769 - +1.1
76,994 + GFF92 0.31 -1.6
77,051 + GFF92 0.38 -0.8
77,051 + GFF92 0.38 -0.3
77,052 - GFF92 0.38 -0.9
77,297 - GFF92 0.66 -1.4
77,304 - GFF92 0.67 -0.8
77,342 + GFF92 0.72 +0.7
77,356 + GFF92 0.73 +0.3
77,357 - GFF92 0.73 +1.0
77,405 - GFF92 0.79 -1.7
77,649 - +0.4
77,789 + GFF93 0.29 +0.1
77,813 + GFF93 0.35 +0.5
77,851 - GFF93 0.45 -4.1
78,060 + -0.2
78,131 - +0.3
78,270 + GFF94 0.17 -0.6
78,335 - GFF94 0.22 +1.5
78,348 + GFF94 0.23 -0.4

Or see this region's nucleotide sequence