Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF91 and GFF92 are separated by 125 nucleotides GFF92 and GFF93 are separated by 94 nucleotides GFF93 and GFF94 are separated by 6 nucleotides
GFF91 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 75,813 to 76,604
GFF91
GFF92 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 76,730 to 77,584
GFF92
GFF93 - Aspartate 1-decarboxylase (EC 4.1.1.11), at 77,679 to 78,059
GFF93
GFF94 - Polysaccharide deacetylase, at 78,066 to 79,295
GFF94
Position (kb)
76
77
78 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 75.773 kb on - strand at 75.773 kb on - strand at 75.796 kb on + strand at 75.907 kb on + strand, within GFF91 at 75.923 kb on + strand, within GFF91 at 75.924 kb on - strand, within GFF91 at 76.005 kb on - strand, within GFF91 at 76.051 kb on + strand, within GFF91 at 76.074 kb on - strand, within GFF91 at 76.248 kb on - strand, within GFF91 at 76.318 kb on + strand, within GFF91 at 76.484 kb on + strand, within GFF91 at 76.548 kb on + strand at 76.548 kb on + strand at 76.548 kb on + strand at 76.549 kb on - strand at 76.549 kb on - strand at 76.550 kb on + strand at 76.551 kb on - strand at 76.551 kb on - strand at 76.551 kb on - strand at 76.679 kb on + strand at 76.734 kb on + strand at 76.769 kb on - strand at 76.994 kb on + strand, within GFF92 at 77.051 kb on + strand, within GFF92 at 77.051 kb on + strand, within GFF92 at 77.052 kb on - strand, within GFF92 at 77.297 kb on - strand, within GFF92 at 77.304 kb on - strand, within GFF92 at 77.342 kb on + strand, within GFF92 at 77.356 kb on + strand, within GFF92 at 77.357 kb on - strand, within GFF92 at 77.405 kb on - strand, within GFF92 at 77.649 kb on - strand at 77.789 kb on + strand, within GFF93 at 77.813 kb on + strand, within GFF93 at 77.851 kb on - strand, within GFF93 at 78.060 kb on + strand at 78.131 kb on - strand at 78.270 kb on + strand, within GFF94 at 78.335 kb on - strand, within GFF94 at 78.348 kb on + strand, within GFF94
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 75,773 - -0.4 75,773 - +2.2 75,796 + +0.9 75,907 + GFF91 0.12 -2.0 75,923 + GFF91 0.14 +0.7 75,924 - GFF91 0.14 +0.6 76,005 - GFF91 0.24 -0.3 76,051 + GFF91 0.30 -0.4 76,074 - GFF91 0.33 +0.8 76,248 - GFF91 0.55 -0.4 76,318 + GFF91 0.64 -2.1 76,484 + GFF91 0.85 -0.1 76,548 + -0.7 76,548 + +0.5 76,548 + -1.6 76,549 - +0.3 76,549 - +1.9 76,550 + +1.5 76,551 - -0.1 76,551 - +0.1 76,551 - -0.7 76,679 + +0.1 76,734 + +1.1 76,769 - +1.1 76,994 + GFF92 0.31 -1.6 77,051 + GFF92 0.38 -0.8 77,051 + GFF92 0.38 -0.3 77,052 - GFF92 0.38 -0.9 77,297 - GFF92 0.66 -1.4 77,304 - GFF92 0.67 -0.8 77,342 + GFF92 0.72 +0.7 77,356 + GFF92 0.73 +0.3 77,357 - GFF92 0.73 +1.0 77,405 - GFF92 0.79 -1.7 77,649 - +0.4 77,789 + GFF93 0.29 +0.1 77,813 + GFF93 0.35 +0.5 77,851 - GFF93 0.45 -4.1 78,060 + -0.2 78,131 - +0.3 78,270 + GFF94 0.17 -0.6 78,335 - GFF94 0.22 +1.5 78,348 + GFF94 0.23 -0.4
Or see this region's nucleotide sequence