Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF913 and GFF914 overlap by 4 nucleotides GFF914 and GFF915 are separated by 16 nucleotides
GFF913 - D-Glucosaminate-6-phosphate ammonia-lyase (EC 4.3.1.-), at 90,937 to 92,055
GFF913
GFF914 - 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14) in D-glucosaminate utilization operon, at 92,052 to 92,792
GFF914
GFF915 - Putative BglB-family transcriptional antiterminator, at 92,809 to 94,722
GFF915
Position (kb)
92
93 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 91.103 kb on - strand, within GFF913 at 91.227 kb on + strand, within GFF913 at 91.228 kb on - strand, within GFF913 at 91.381 kb on - strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.405 kb on + strand, within GFF913 at 91.408 kb on - strand, within GFF913 at 91.427 kb on + strand, within GFF913 at 91.480 kb on - strand, within GFF913 at 91.554 kb on + strand, within GFF913 at 91.558 kb on + strand, within GFF913 at 91.632 kb on - strand, within GFF913 at 91.752 kb on - strand, within GFF913 at 91.811 kb on + strand, within GFF913 at 91.921 kb on + strand, within GFF913 at 91.930 kb on + strand, within GFF913 at 91.931 kb on - strand, within GFF913 at 92.001 kb on - strand at 92.220 kb on + strand, within GFF914 at 92.232 kb on - strand, within GFF914 at 92.311 kb on + strand, within GFF914 at 92.482 kb on + strand, within GFF914 at 92.568 kb on + strand, within GFF914 at 92.569 kb on - strand, within GFF914 at 92.570 kb on + strand, within GFF914 at 92.571 kb on - strand, within GFF914 at 92.578 kb on + strand, within GFF914 at 92.681 kb on - strand, within GFF914 at 92.703 kb on + strand, within GFF914 at 92.706 kb on - strand, within GFF914 at 92.706 kb on - strand, within GFF914 at 92.720 kb on + strand at 92.720 kb on + strand at 92.761 kb on + strand at 92.761 kb on + strand at 93.090 kb on - strand, within GFF915 at 93.094 kb on + strand, within GFF915 at 93.095 kb on - strand, within GFF915 at 93.308 kb on + strand, within GFF915 at 93.308 kb on + strand, within GFF915 at 93.309 kb on - strand, within GFF915 at 93.351 kb on + strand, within GFF915 at 93.352 kb on - strand, within GFF915 at 93.573 kb on - strand, within GFF915 at 93.575 kb on + strand, within GFF915 at 93.614 kb on + strand, within GFF915 at 93.634 kb on - strand, within GFF915 at 93.634 kb on - strand, within GFF915
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 91,103 - GFF913 0.15 +0.8 91,227 + GFF913 0.26 +1.5 91,228 - GFF913 0.26 +0.4 91,381 - GFF913 0.40 +1.2 91,405 + GFF913 0.42 -0.1 91,405 + GFF913 0.42 -0.1 91,405 + GFF913 0.42 -0.1 91,408 - GFF913 0.42 +0.6 91,427 + GFF913 0.44 -0.7 91,480 - GFF913 0.49 +1.8 91,554 + GFF913 0.55 -0.5 91,558 + GFF913 0.55 +0.1 91,632 - GFF913 0.62 +0.5 91,752 - GFF913 0.73 +1.0 91,811 + GFF913 0.78 +1.3 91,921 + GFF913 0.88 -0.0 91,930 + GFF913 0.89 -1.0 91,931 - GFF913 0.89 +0.3 92,001 - -0.6 92,220 + GFF914 0.23 -0.4 92,232 - GFF914 0.24 +0.4 92,311 + GFF914 0.35 -0.4 92,482 + GFF914 0.58 +0.1 92,568 + GFF914 0.70 -0.6 92,569 - GFF914 0.70 -0.2 92,570 + GFF914 0.70 -2.0 92,571 - GFF914 0.70 -3.2 92,578 + GFF914 0.71 +1.2 92,681 - GFF914 0.85 -1.1 92,703 + GFF914 0.88 -3.8 92,706 - GFF914 0.88 +0.4 92,706 - GFF914 0.88 +0.1 92,720 + -0.0 92,720 + +1.0 92,761 + -0.3 92,761 + -0.4 93,090 - GFF915 0.15 -2.4 93,094 + GFF915 0.15 +0.2 93,095 - GFF915 0.15 -0.2 93,308 + GFF915 0.26 +1.1 93,308 + GFF915 0.26 +0.0 93,309 - GFF915 0.26 -0.1 93,351 + GFF915 0.28 +0.6 93,352 - GFF915 0.28 -0.2 93,573 - GFF915 0.40 -0.5 93,575 + GFF915 0.40 -1.3 93,614 + GFF915 0.42 +0.2 93,634 - GFF915 0.43 -3.3 93,634 - GFF915 0.43 +2.2
Or see this region's nucleotide sequence