Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF54 and GFF55 are separated by 236 nucleotides GFF55 and GFF56 are separated by 29 nucleotides GFF56 and GFF57 are separated by 36 nucleotides
GFF54 - Methionine aminopeptidase (EC 3.4.11.18), at 35,243 to 36,037
GFF54
GFF55 - [Protein-PII] uridylyltransferase (EC 2.7.7.59), at 36,274 to 38,946
GFF55
GFF56 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 38,976 to 39,800
GFF56
GFF57 - Permeases of the major facilitator superfamily, at 39,837 to 41,138
GFF57
Position (kb) 36
37
38
39 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 36.036 kb on - strand at 36.149 kb on + strand at 36.149 kb on + strand at 36.150 kb on - strand at 36.150 kb on - strand at 36.153 kb on + strand at 36.153 kb on + strand at 36.153 kb on + strand at 36.157 kb on + strand at 36.157 kb on + strand at 36.189 kb on + strand at 36.190 kb on - strand at 36.214 kb on - strand at 36.260 kb on - strand at 36.279 kb on + strand at 36.694 kb on - strand, within GFF55 at 36.694 kb on - strand, within GFF55 at 36.698 kb on + strand, within GFF55 at 36.699 kb on - strand, within GFF55 at 36.817 kb on - strand, within GFF55 at 36.912 kb on + strand, within GFF55 at 36.930 kb on + strand, within GFF55 at 36.963 kb on + strand, within GFF55 at 36.963 kb on + strand, within GFF55 at 36.963 kb on + strand, within GFF55 at 37.220 kb on - strand, within GFF55 at 37.353 kb on + strand, within GFF55 at 37.416 kb on + strand, within GFF55 at 37.510 kb on + strand, within GFF55 at 37.511 kb on - strand, within GFF55 at 37.542 kb on + strand, within GFF55 at 37.601 kb on + strand, within GFF55 at 37.612 kb on + strand, within GFF55 at 37.612 kb on + strand, within GFF55 at 37.612 kb on + strand, within GFF55 at 37.612 kb on + strand, within GFF55 at 37.612 kb on + strand, within GFF55 at 37.613 kb on - strand, within GFF55 at 37.614 kb on + strand, within GFF55 at 37.614 kb on + strand, within GFF55 at 37.614 kb on + strand, within GFF55 at 37.614 kb on + strand, within GFF55 at 37.670 kb on - strand, within GFF55 at 37.869 kb on - strand, within GFF55 at 37.947 kb on - strand, within GFF55 at 38.094 kb on + strand, within GFF55 at 38.333 kb on + strand, within GFF55 at 38.471 kb on + strand, within GFF55 at 38.523 kb on + strand, within GFF55 at 38.523 kb on + strand, within GFF55 at 38.530 kb on - strand, within GFF55 at 38.616 kb on + strand, within GFF55 at 38.667 kb on + strand, within GFF55 at 38.877 kb on + strand at 38.878 kb on - strand at 38.880 kb on + strand at 38.957 kb on + strand at 38.959 kb on + strand at 39.792 kb on - strand at 39.938 kb on + strand at 39.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 36,036 - -2.6 36,149 + -3.0 36,149 + +0.4 36,150 - +1.5 36,150 - -1.5 36,153 + +0.5 36,153 + +1.1 36,153 + -0.2 36,157 + +1.3 36,157 + -0.9 36,189 + +0.5 36,190 - -5.0 36,214 - -1.2 36,260 - -1.8 36,279 + -1.7 36,694 - GFF55 0.16 -1.9 36,694 - GFF55 0.16 -2.9 36,698 + GFF55 0.16 -3.5 36,699 - GFF55 0.16 -1.8 36,817 - GFF55 0.20 -2.6 36,912 + GFF55 0.24 -3.8 36,930 + GFF55 0.25 -4.0 36,963 + GFF55 0.26 -2.6 36,963 + GFF55 0.26 -0.5 36,963 + GFF55 0.26 -3.9 37,220 - GFF55 0.35 -0.5 37,353 + GFF55 0.40 +0.9 37,416 + GFF55 0.43 -2.0 37,510 + GFF55 0.46 -0.5 37,511 - GFF55 0.46 -4.0 37,542 + GFF55 0.47 -3.1 37,601 + GFF55 0.50 -0.5 37,612 + GFF55 0.50 -3.3 37,612 + GFF55 0.50 -1.7 37,612 + GFF55 0.50 -0.3 37,612 + GFF55 0.50 -1.0 37,612 + GFF55 0.50 -1.4 37,613 - GFF55 0.50 -3.9 37,614 + GFF55 0.50 -1.3 37,614 + GFF55 0.50 -3.2 37,614 + GFF55 0.50 -4.0 37,614 + GFF55 0.50 -1.9 37,670 - GFF55 0.52 -3.8 37,869 - GFF55 0.60 -1.5 37,947 - GFF55 0.63 -3.4 38,094 + GFF55 0.68 -3.9 38,333 + GFF55 0.77 -4.4 38,471 + GFF55 0.82 -2.2 38,523 + GFF55 0.84 -2.8 38,523 + GFF55 0.84 -2.5 38,530 - GFF55 0.84 -2.7 38,616 + GFF55 0.88 -2.5 38,667 + GFF55 0.90 -2.7 38,877 + -2.3 38,878 - -2.7 38,880 + -1.0 38,957 + +1.2 38,959 + +0.8 39,792 - -1.6 39,938 + +0.5 39,944 - -1.1
Or see this region's nucleotide sequence