Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF509 and GFF510 are separated by 63 nucleotides GFF510 and GFF511 are separated by 53 nucleotides
GFF509 - Putative phosphosugar-binding protein, at 238,728 to 239,702
GFF509
GFF510 - kinase (ribokinase family), at 239,766 to 240,611
GFF510
GFF511 - anaerobic C4-dicarboxylate transporter, at 240,665 to 241,984
GFF511
Position (kb) 239
240
241 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 238.788 kb on - strand at 238.801 kb on - strand at 238.907 kb on + strand, within GFF509 at 238.968 kb on - strand, within GFF509 at 238.985 kb on + strand, within GFF509 at 239.073 kb on + strand, within GFF509 at 239.074 kb on - strand, within GFF509 at 239.108 kb on + strand, within GFF509 at 239.280 kb on + strand, within GFF509 at 239.282 kb on + strand, within GFF509 at 239.342 kb on - strand, within GFF509 at 239.390 kb on + strand, within GFF509 at 239.589 kb on + strand, within GFF509 at 239.628 kb on + strand at 239.700 kb on + strand at 239.740 kb on - strand at 239.744 kb on + strand at 239.811 kb on + strand at 239.844 kb on - strand at 239.865 kb on + strand, within GFF510 at 239.936 kb on - strand, within GFF510 at 239.963 kb on - strand, within GFF510 at 240.418 kb on - strand, within GFF510 at 240.614 kb on + strand at 240.615 kb on - strand at 240.664 kb on + strand at 240.664 kb on + strand at 240.681 kb on + strand at 240.823 kb on + strand, within GFF511 at 240.856 kb on - strand, within GFF511 at 240.856 kb on - strand, within GFF511 at 240.923 kb on + strand, within GFF511 at 240.929 kb on - strand, within GFF511 at 240.929 kb on - strand, within GFF511 at 240.934 kb on - strand, within GFF511 at 240.953 kb on + strand, within GFF511 at 241.057 kb on - strand, within GFF511 at 241.103 kb on - strand, within GFF511 at 241.169 kb on - strand, within GFF511 at 241.229 kb on - strand, within GFF511 at 241.288 kb on - strand, within GFF511 at 241.507 kb on - strand, within GFF511
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 238,788 - -0.2 238,801 - +0.2 238,907 + GFF509 0.18 -0.1 238,968 - GFF509 0.25 +0.6 238,985 + GFF509 0.26 +0.0 239,073 + GFF509 0.35 +1.1 239,074 - GFF509 0.35 -0.6 239,108 + GFF509 0.39 +0.7 239,280 + GFF509 0.57 +0.2 239,282 + GFF509 0.57 -0.7 239,342 - GFF509 0.63 +0.5 239,390 + GFF509 0.68 -0.7 239,589 + GFF509 0.88 -0.2 239,628 + -2.1 239,700 + -1.4 239,740 - -0.9 239,744 + +0.5 239,811 + +0.2 239,844 - -0.4 239,865 + GFF510 0.12 -2.4 239,936 - GFF510 0.20 -2.4 239,963 - GFF510 0.23 -2.2 240,418 - GFF510 0.77 -0.7 240,614 + +0.9 240,615 - -0.1 240,664 + -0.7 240,664 + -2.2 240,681 + +0.1 240,823 + GFF511 0.12 -0.2 240,856 - GFF511 0.14 -0.4 240,856 - GFF511 0.14 -1.7 240,923 + GFF511 0.20 +0.6 240,929 - GFF511 0.20 -3.6 240,929 - GFF511 0.20 -0.0 240,934 - GFF511 0.20 +0.5 240,953 + GFF511 0.22 -0.4 241,057 - GFF511 0.30 -0.0 241,103 - GFF511 0.33 -0.1 241,169 - GFF511 0.38 +0.4 241,229 - GFF511 0.43 -0.1 241,288 - GFF511 0.47 -1.6 241,507 - GFF511 0.64 +0.1
Or see this region's nucleotide sequence