Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4679 and GFF4680 are separated by 45 nucleotides GFF4680 and GFF4681 are separated by 62 nucleotides GFF4681 and GFF4682 overlap by 20 nucleotides GFF4682 and GFF4683 overlap by 4 nucleotides GFF4683 and GFF4684 are separated by 98 nucleotides
GFF4679 - Phage tail assembly, at 8,724 to 9,473
GFF4679
GFF4680 - Phage minor tail protein, at 9,519 to 9,917
GFF4680
GFF4681 - Phage minor tail protein, at 9,980 to 10,243
GFF4681
GFF4682 - Phage tail length tape-measure protein 1, at 10,224 to 13,310
GFF4682
GFF4683 - Phage minor tail protein, at 13,307 to 13,639
GFF4683
GFF4684 - Attachment invasion locus protein precursor, at 13,738 to 14,235
GFF4684
Position (kb) 10
11
12
13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 9.382 kb on + strand, within GFF4679 at 9.392 kb on + strand, within GFF4679 at 9.514 kb on - strand at 9.514 kb on - strand at 9.593 kb on + strand, within GFF4680 at 9.594 kb on - strand, within GFF4680 at 9.623 kb on + strand, within GFF4680 at 9.634 kb on - strand, within GFF4680 at 9.681 kb on + strand, within GFF4680 at 9.681 kb on + strand, within GFF4680 at 9.682 kb on - strand, within GFF4680 at 9.682 kb on - strand, within GFF4680 at 10.268 kb on + strand at 10.460 kb on + strand at 10.610 kb on - strand, within GFF4682 at 10.610 kb on - strand, within GFF4682 at 10.972 kb on + strand, within GFF4682 at 11.132 kb on + strand, within GFF4682 at 11.143 kb on - strand, within GFF4682 at 11.183 kb on + strand, within GFF4682 at 11.285 kb on - strand, within GFF4682 at 11.584 kb on + strand, within GFF4682 at 11.585 kb on - strand, within GFF4682 at 11.585 kb on - strand, within GFF4682 at 11.853 kb on + strand, within GFF4682 at 11.964 kb on + strand, within GFF4682 at 12.018 kb on - strand, within GFF4682 at 12.058 kb on - strand, within GFF4682 at 12.534 kb on + strand, within GFF4682 at 12.534 kb on + strand, within GFF4682 at 12.879 kb on - strand, within GFF4682 at 12.928 kb on - strand, within GFF4682 at 12.975 kb on + strand, within GFF4682 at 13.010 kb on + strand at 13.083 kb on - strand at 13.239 kb on - strand at 13.544 kb on + strand, within GFF4683 at 13.555 kb on + strand, within GFF4683 at 13.666 kb on + strand at 13.782 kb on + strand at 13.840 kb on + strand, within GFF4684 at 13.941 kb on + strand, within GFF4684 at 13.942 kb on - strand, within GFF4684 at 13.945 kb on + strand, within GFF4684 at 13.946 kb on - strand, within GFF4684 at 13.965 kb on + strand, within GFF4684 at 13.969 kb on + strand, within GFF4684 at 13.976 kb on - strand, within GFF4684 at 13.977 kb on + strand, within GFF4684 at 13.997 kb on - strand, within GFF4684 at 13.998 kb on + strand, within GFF4684 at 14.039 kb on - strand, within GFF4684 at 14.088 kb on + strand, within GFF4684 at 14.187 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 9,382 + GFF4679 0.88 -1.8 9,392 + GFF4679 0.89 +0.0 9,514 - -0.3 9,514 - +0.2 9,593 + GFF4680 0.19 +0.3 9,594 - GFF4680 0.19 -1.6 9,623 + GFF4680 0.26 -0.7 9,634 - GFF4680 0.29 -0.1 9,681 + GFF4680 0.41 +0.4 9,681 + GFF4680 0.41 +0.3 9,682 - GFF4680 0.41 +1.4 9,682 - GFF4680 0.41 +0.4 10,268 + -0.9 10,460 + -3.5 10,610 - GFF4682 0.13 -0.9 10,610 - GFF4682 0.13 -2.4 10,972 + GFF4682 0.24 +0.0 11,132 + GFF4682 0.29 -3.6 11,143 - GFF4682 0.30 -0.4 11,183 + GFF4682 0.31 -1.9 11,285 - GFF4682 0.34 -0.8 11,584 + GFF4682 0.44 -0.7 11,585 - GFF4682 0.44 +0.8 11,585 - GFF4682 0.44 -0.2 11,853 + GFF4682 0.53 +0.8 11,964 + GFF4682 0.56 +4.0 12,018 - GFF4682 0.58 -0.8 12,058 - GFF4682 0.59 +0.1 12,534 + GFF4682 0.75 -1.3 12,534 + GFF4682 0.75 +0.5 12,879 - GFF4682 0.86 +0.4 12,928 - GFF4682 0.88 -0.1 12,975 + GFF4682 0.89 +0.3 13,010 + +0.7 13,083 - +1.4 13,239 - -0.8 13,544 + GFF4683 0.71 +0.1 13,555 + GFF4683 0.74 +0.1 13,666 + -0.1 13,782 + +0.1 13,840 + GFF4684 0.20 -2.5 13,941 + GFF4684 0.41 +0.2 13,942 - GFF4684 0.41 -0.8 13,945 + GFF4684 0.42 -1.0 13,946 - GFF4684 0.42 +1.1 13,965 + GFF4684 0.46 -0.1 13,969 + GFF4684 0.46 +0.1 13,976 - GFF4684 0.48 +0.1 13,977 + GFF4684 0.48 -1.0 13,997 - GFF4684 0.52 +1.2 13,998 + GFF4684 0.52 -2.5 14,039 - GFF4684 0.60 +0.3 14,088 + GFF4684 0.70 -0.6 14,187 + +0.8
Or see this region's nucleotide sequence