Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4592 and GFF4593 are separated by 50 nucleotides GFF4593 and GFF4594 overlap by 14 nucleotides
GFF4592 - TcuC: integral membrane protein used to transport tricarballylate across the cell membrane, at 381,915 to 383,219
GFF4592
GFF4593 - TcuB: works with TcuA to oxidize tricarballylate to cis-aconitate, at 383,270 to 384,409
GFF4593
GFF4594 - TcuA: flavoprotein used to oxidize tricarballylate to cis-aconitate, at 384,396 to 385,799
GFF4594
Position (kb) 383
384
385 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 382.289 kb on + strand, within GFF4592 at 382.290 kb on - strand, within GFF4592 at 382.456 kb on + strand, within GFF4592 at 382.456 kb on + strand, within GFF4592 at 382.503 kb on - strand, within GFF4592 at 382.689 kb on - strand, within GFF4592 at 382.697 kb on + strand, within GFF4592 at 382.799 kb on - strand, within GFF4592 at 382.984 kb on + strand, within GFF4592 at 383.101 kb on + strand at 383.101 kb on + strand at 383.102 kb on - strand at 383.141 kb on - strand at 383.257 kb on - strand at 383.662 kb on - strand, within GFF4593 at 383.705 kb on - strand, within GFF4593 at 383.886 kb on - strand, within GFF4593 at 383.950 kb on + strand, within GFF4593 at 384.084 kb on + strand, within GFF4593 at 384.220 kb on + strand, within GFF4593 at 384.241 kb on - strand, within GFF4593 at 384.445 kb on - strand at 384.614 kb on + strand, within GFF4594 at 384.646 kb on + strand, within GFF4594 at 384.763 kb on + strand, within GFF4594 at 384.831 kb on - strand, within GFF4594 at 385.158 kb on - strand, within GFF4594 at 385.269 kb on + strand, within GFF4594 at 385.400 kb on - strand, within GFF4594
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 382,289 + GFF4592 0.29 +0.1 382,290 - GFF4592 0.29 +0.8 382,456 + GFF4592 0.41 -0.6 382,456 + GFF4592 0.41 -1.6 382,503 - GFF4592 0.45 +0.5 382,689 - GFF4592 0.59 -2.4 382,697 + GFF4592 0.60 -1.2 382,799 - GFF4592 0.68 -0.3 382,984 + GFF4592 0.82 -0.9 383,101 + +1.5 383,101 + -1.7 383,102 - -0.8 383,141 - -4.1 383,257 - +0.5 383,662 - GFF4593 0.34 -2.6 383,705 - GFF4593 0.38 +0.5 383,886 - GFF4593 0.54 +0.9 383,950 + GFF4593 0.60 -0.8 384,084 + GFF4593 0.71 +2.0 384,220 + GFF4593 0.83 +0.2 384,241 - GFF4593 0.85 -0.2 384,445 - -0.3 384,614 + GFF4594 0.16 -0.4 384,646 + GFF4594 0.18 +1.3 384,763 + GFF4594 0.26 -0.2 384,831 - GFF4594 0.31 +0.7 385,158 - GFF4594 0.54 -1.3 385,269 + GFF4594 0.62 -0.3 385,400 - GFF4594 0.72 +0.4
Or see this region's nucleotide sequence