Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4521 and GFF4522 are separated by 82 nucleotides GFF4522 and GFF4523 are separated by 234 nucleotides GFF4523 and GFF4524 are separated by 109 nucleotides
GFF4521 - Uncharacterized zinc-type alcohol dehydrogenase-like protein ybdR, at 308,237 to 309,475
GFF4521
GFF4522 - Regulator of nucleoside diphosphate kinase, at 309,558 to 309,968
GFF4522
GFF4523 - Ribonuclease I precursor (EC 3.1.27.6), at 310,203 to 311,009
GFF4523
GFF4524 - Citrate Succinate antiporter (TC 2.A.47.3.2), at 311,119 to 312,582
GFF4524
Position (kb) 310
311
312 Strain fitness (log2 ratio) -2
-1
0
1
2 at 309.317 kb on + strand, within GFF4521 at 309.317 kb on + strand, within GFF4521 at 309.318 kb on - strand, within GFF4521 at 309.318 kb on - strand, within GFF4521 at 309.319 kb on + strand, within GFF4521 at 309.319 kb on + strand, within GFF4521 at 309.319 kb on + strand, within GFF4521 at 309.320 kb on - strand, within GFF4521 at 309.546 kb on - strand at 309.896 kb on - strand, within GFF4522 at 309.924 kb on - strand, within GFF4522 at 309.951 kb on - strand at 309.989 kb on + strand at 310.510 kb on + strand, within GFF4523 at 310.900 kb on + strand, within GFF4523 at 311.026 kb on + strand at 311.053 kb on - strand at 311.170 kb on - strand at 311.172 kb on - strand at 311.172 kb on - strand at 311.172 kb on - strand at 311.205 kb on - strand at 311.284 kb on - strand, within GFF4524 at 311.308 kb on - strand, within GFF4524 at 311.405 kb on + strand, within GFF4524 at 311.406 kb on - strand, within GFF4524 at 311.476 kb on - strand, within GFF4524 at 311.544 kb on + strand, within GFF4524 at 311.812 kb on + strand, within GFF4524 at 311.817 kb on - strand, within GFF4524 at 311.930 kb on - strand, within GFF4524 at 311.932 kb on + strand, within GFF4524 at 311.932 kb on + strand, within GFF4524
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 309,317 + GFF4521 0.87 +0.3 309,317 + GFF4521 0.87 +1.1 309,318 - GFF4521 0.87 +0.6 309,318 - GFF4521 0.87 -2.7 309,319 + GFF4521 0.87 +1.5 309,319 + GFF4521 0.87 +0.5 309,319 + GFF4521 0.87 -0.0 309,320 - GFF4521 0.87 -0.6 309,546 - +0.7 309,896 - GFF4522 0.82 -1.3 309,924 - GFF4522 0.89 -0.6 309,951 - +0.7 309,989 + +0.6 310,510 + GFF4523 0.38 -1.7 310,900 + GFF4523 0.86 -0.3 311,026 + +0.5 311,053 - +0.9 311,170 - -0.6 311,172 - +0.0 311,172 - +0.2 311,172 - +0.8 311,205 - +1.1 311,284 - GFF4524 0.11 +0.7 311,308 - GFF4524 0.13 -1.7 311,405 + GFF4524 0.20 -0.4 311,406 - GFF4524 0.20 +1.6 311,476 - GFF4524 0.24 +1.5 311,544 + GFF4524 0.29 +2.4 311,812 + GFF4524 0.47 -0.9 311,817 - GFF4524 0.48 +0.6 311,930 - GFF4524 0.55 +0.3 311,932 + GFF4524 0.56 +0.4 311,932 + GFF4524 0.56 -0.8
Or see this region's nucleotide sequence