Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4512 and GFF4513 are separated by 380 nucleotides GFF4513 and GFF4514 are separated by 241 nucleotides
GFF4512 - Thiol:disulfide interchange protein DsbG precursor, at 299,476 to 300,282
GFF4512
GFF4513 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 300,663 to 301,226
GFF4513
GFF4514 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 301,468 to 303,033
GFF4514
Position (kb) 300
301
302 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 299.867 kb on + strand, within GFF4512 at 299.867 kb on + strand, within GFF4512 at 299.868 kb on - strand, within GFF4512 at 299.868 kb on - strand, within GFF4512 at 299.955 kb on - strand, within GFF4512 at 300.005 kb on + strand, within GFF4512 at 300.006 kb on - strand, within GFF4512 at 300.009 kb on + strand, within GFF4512 at 300.009 kb on + strand, within GFF4512 at 300.009 kb on + strand, within GFF4512 at 300.011 kb on + strand, within GFF4512 at 300.048 kb on - strand, within GFF4512 at 300.196 kb on - strand, within GFF4512 at 300.217 kb on - strand at 300.282 kb on + strand at 300.283 kb on - strand at 300.283 kb on - strand at 300.347 kb on + strand at 300.348 kb on - strand at 300.392 kb on + strand at 300.394 kb on + strand at 300.394 kb on + strand at 300.395 kb on - strand at 300.423 kb on - strand at 300.568 kb on - strand at 300.791 kb on + strand, within GFF4513 at 300.834 kb on + strand, within GFF4513 at 300.835 kb on - strand, within GFF4513 at 300.943 kb on - strand, within GFF4513 at 301.156 kb on + strand, within GFF4513 at 301.285 kb on + strand at 301.285 kb on + strand at 301.483 kb on - strand at 301.635 kb on + strand, within GFF4514 at 301.668 kb on + strand, within GFF4514 at 301.915 kb on - strand, within GFF4514 at 302.022 kb on + strand, within GFF4514 at 302.022 kb on + strand, within GFF4514 at 302.022 kb on + strand, within GFF4514 at 302.104 kb on + strand, within GFF4514 at 302.105 kb on - strand, within GFF4514 at 302.112 kb on - strand, within GFF4514
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 299,867 + GFF4512 0.48 +1.2 299,867 + GFF4512 0.48 +0.3 299,868 - GFF4512 0.49 -0.3 299,868 - GFF4512 0.49 +0.7 299,955 - GFF4512 0.59 +1.0 300,005 + GFF4512 0.66 +0.1 300,006 - GFF4512 0.66 +0.9 300,009 + GFF4512 0.66 +0.2 300,009 + GFF4512 0.66 +2.5 300,009 + GFF4512 0.66 -4.6 300,011 + GFF4512 0.66 +1.0 300,048 - GFF4512 0.71 +1.2 300,196 - GFF4512 0.89 -0.0 300,217 - +0.4 300,282 + +0.4 300,283 - +0.0 300,283 - -1.1 300,347 + -0.1 300,348 - +0.5 300,392 + +0.3 300,394 + +0.6 300,394 + +0.9 300,395 - -1.0 300,423 - -0.5 300,568 - +1.0 300,791 + GFF4513 0.23 +0.0 300,834 + GFF4513 0.30 -1.8 300,835 - GFF4513 0.30 -6.4 300,943 - GFF4513 0.50 -2.1 301,156 + GFF4513 0.87 -0.5 301,285 + +0.7 301,285 + +0.8 301,483 - -1.7 301,635 + GFF4514 0.11 +0.0 301,668 + GFF4514 0.13 -0.1 301,915 - GFF4514 0.29 -2.5 302,022 + GFF4514 0.35 +0.4 302,022 + GFF4514 0.35 -0.1 302,022 + GFF4514 0.35 +0.2 302,104 + GFF4514 0.41 +0.5 302,105 - GFF4514 0.41 -0.8 302,112 - GFF4514 0.41 -0.7
Or see this region's nucleotide sequence