Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4447 and GFF4448 are separated by 546 nucleotides GFF4448 and GFF4449 are separated by 114 nucleotides GFF4449 and GFF4450 are separated by 43 nucleotides
GFF4447 - Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9), at 232,916 to 233,782
GFF4447
GFF4448 - Fimbriae-like adhesin SfmA, at 234,329 to 234,886
GFF4448
GFF4449 - Fimbriae-like adhesin FimI, at 235,001 to 235,495
GFF4449
GFF4450 - Fimbrial periplasmic chaperone SfmC, at 235,539 to 236,231
GFF4450
Position (kb) 234
235 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 234.479 kb on - strand, within GFF4448 at 234.541 kb on + strand, within GFF4448 at 234.545 kb on - strand, within GFF4448 at 234.721 kb on + strand, within GFF4448 at 234.722 kb on - strand, within GFF4448 at 234.722 kb on - strand, within GFF4448 at 234.812 kb on + strand, within GFF4448 at 234.868 kb on + strand at 234.947 kb on + strand at 234.956 kb on + strand at 234.956 kb on + strand at 234.956 kb on + strand at 234.956 kb on + strand at 234.956 kb on + strand at 234.957 kb on - strand at 234.957 kb on - strand at 235.127 kb on + strand, within GFF4449 at 235.350 kb on + strand, within GFF4449 at 235.354 kb on + strand, within GFF4449 at 235.355 kb on - strand, within GFF4449 at 235.477 kb on + strand at 235.625 kb on - strand, within GFF4450 at 235.682 kb on + strand, within GFF4450 at 235.685 kb on + strand, within GFF4450 at 235.814 kb on + strand, within GFF4450
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 234,479 - GFF4448 0.27 -2.4 234,541 + GFF4448 0.38 -2.3 234,545 - GFF4448 0.39 +0.1 234,721 + GFF4448 0.70 -0.8 234,722 - GFF4448 0.70 +1.4 234,722 - GFF4448 0.70 -2.9 234,812 + GFF4448 0.87 +0.9 234,868 + +0.3 234,947 + +0.2 234,956 + -0.1 234,956 + -1.4 234,956 + +0.5 234,956 + +0.8 234,956 + +0.2 234,957 - +0.4 234,957 - +1.4 235,127 + GFF4449 0.25 -0.3 235,350 + GFF4449 0.71 +0.1 235,354 + GFF4449 0.71 -0.2 235,355 - GFF4449 0.72 +1.3 235,477 + -1.7 235,625 - GFF4450 0.12 -2.1 235,682 + GFF4450 0.21 -1.9 235,685 + GFF4450 0.21 +1.2 235,814 + GFF4450 0.40 +0.6
Or see this region's nucleotide sequence