Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4428 and GFF4429 are separated by 58 nucleotides GFF4429 and GFF4430 are separated by 22 nucleotides
GFF4428 - Allantoinase (EC 3.5.2.5), at 214,172 to 215,533
GFF4428
GFF4429 - Xanthine permease, at 215,592 to 216,890
GFF4429
GFF4430 - Glycerate kinase (EC 2.7.1.31), at 216,913 to 218,061
GFF4430
Position (kb) 215
216
217 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 214.725 kb on + strand, within GFF4428 at 214.726 kb on - strand, within GFF4428 at 214.802 kb on + strand, within GFF4428 at 214.802 kb on + strand, within GFF4428 at 214.802 kb on + strand, within GFF4428 at 214.803 kb on - strand, within GFF4428 at 215.234 kb on - strand, within GFF4428 at 215.308 kb on + strand, within GFF4428 at 215.309 kb on - strand, within GFF4428 at 215.342 kb on - strand, within GFF4428 at 215.361 kb on - strand, within GFF4428 at 215.361 kb on - strand, within GFF4428 at 215.361 kb on - strand, within GFF4428 at 215.388 kb on - strand, within GFF4428 at 215.422 kb on + strand at 215.438 kb on - strand at 215.468 kb on + strand at 215.469 kb on - strand at 215.469 kb on - strand at 215.531 kb on + strand at 215.580 kb on + strand at 215.604 kb on - strand at 215.832 kb on - strand, within GFF4429 at 215.954 kb on - strand, within GFF4429 at 216.077 kb on + strand, within GFF4429 at 216.168 kb on - strand, within GFF4429 at 216.245 kb on - strand, within GFF4429 at 216.309 kb on - strand, within GFF4429 at 216.380 kb on + strand, within GFF4429 at 216.496 kb on + strand, within GFF4429 at 216.497 kb on - strand, within GFF4429 at 216.586 kb on + strand, within GFF4429 at 216.720 kb on + strand, within GFF4429 at 216.819 kb on - strand at 216.982 kb on - strand at 217.001 kb on + strand at 217.002 kb on - strand at 217.173 kb on + strand, within GFF4430 at 217.299 kb on + strand, within GFF4430 at 217.497 kb on + strand, within GFF4430 at 217.593 kb on + strand, within GFF4430 at 217.597 kb on + strand, within GFF4430
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 214,725 + GFF4428 0.41 -0.9 214,726 - GFF4428 0.41 -0.1 214,802 + GFF4428 0.46 +0.4 214,802 + GFF4428 0.46 +0.2 214,802 + GFF4428 0.46 +1.6 214,803 - GFF4428 0.46 -3.2 215,234 - GFF4428 0.78 -2.7 215,308 + GFF4428 0.83 +0.3 215,309 - GFF4428 0.83 -0.2 215,342 - GFF4428 0.86 -0.3 215,361 - GFF4428 0.87 -0.4 215,361 - GFF4428 0.87 +1.4 215,361 - GFF4428 0.87 +0.6 215,388 - GFF4428 0.89 -1.3 215,422 + +0.9 215,438 - -1.5 215,468 + -0.9 215,469 - +0.7 215,469 - -0.0 215,531 + +0.7 215,580 + +1.9 215,604 - +1.3 215,832 - GFF4429 0.18 -0.4 215,954 - GFF4429 0.28 +0.0 216,077 + GFF4429 0.37 -1.2 216,168 - GFF4429 0.44 -2.5 216,245 - GFF4429 0.50 -1.0 216,309 - GFF4429 0.55 +0.1 216,380 + GFF4429 0.61 +0.6 216,496 + GFF4429 0.70 -2.0 216,497 - GFF4429 0.70 +0.1 216,586 + GFF4429 0.77 -0.7 216,720 + GFF4429 0.87 -0.7 216,819 - -1.4 216,982 - +0.5 217,001 + +1.2 217,002 - +0.5 217,173 + GFF4430 0.23 +0.5 217,299 + GFF4430 0.34 +0.7 217,497 + GFF4430 0.51 +0.0 217,593 + GFF4430 0.59 -0.6 217,597 + GFF4430 0.60 -1.3
Or see this region's nucleotide sequence