Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4340 and GFF4341 are separated by 11 nucleotides GFF4341 and GFF4342 are separated by 81 nucleotides GFF4342 and GFF4343 are separated by 14 nucleotides
GFF4340 - corresponds to STY0478 from Accession AL513382: Salmonella typhi CT18, at 120,468 to 121,979
GFF4340
GFF4341 - Probable secreted protein, at 121,991 to 123,484
GFF4341
GFF4342 - Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB, at 123,566 to 124,453
GFF4342
GFF4343 - Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-), at 124,468 to 124,797
GFF4343
Position (kb) 121
122
123
124 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 121.027 kb on + strand, within GFF4340 at 122.439 kb on - strand, within GFF4341 at 122.837 kb on - strand, within GFF4341 at 123.391 kb on + strand at 123.463 kb on - strand at 123.577 kb on - strand at 123.607 kb on + strand at 123.652 kb on + strand at 123.686 kb on - strand, within GFF4342 at 123.823 kb on - strand, within GFF4342 at 124.078 kb on - strand, within GFF4342 at 124.078 kb on - strand, within GFF4342 at 124.086 kb on + strand, within GFF4342 at 124.194 kb on - strand, within GFF4342 at 124.289 kb on - strand, within GFF4342 at 124.289 kb on - strand, within GFF4342 at 124.334 kb on - strand, within GFF4342 at 124.336 kb on - strand, within GFF4342
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 121,027 + GFF4340 0.37 -1.1 122,439 - GFF4341 0.30 -4.6 122,837 - GFF4341 0.57 -2.0 123,391 + +0.1 123,463 - -2.4 123,577 - -0.0 123,607 + +0.2 123,652 + -0.4 123,686 - GFF4342 0.14 +0.1 123,823 - GFF4342 0.29 -1.0 124,078 - GFF4342 0.58 -0.8 124,078 - GFF4342 0.58 -0.2 124,086 + GFF4342 0.59 -3.8 124,194 - GFF4342 0.71 -1.0 124,289 - GFF4342 0.81 -0.7 124,289 - GFF4342 0.81 +0.1 124,334 - GFF4342 0.86 +0.5 124,336 - GFF4342 0.87 +0.4
Or see this region's nucleotide sequence