Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4304 and GFF4305 are separated by 196 nucleotides GFF4305 and GFF4306 are separated by 22 nucleotides GFF4306 and GFF4307 are separated by 60 nucleotides
GFF4304 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 89,772 to 90,836
GFF4304
GFF4305 - tRNA-guanine transglycosylase (EC 2.4.2.29), at 91,033 to 92,160
GFF4305
GFF4306 - Preprotein translocase subunit YajC (TC 3.A.5.1.1), at 92,183 to 92,515
GFF4306
GFF4307 - Protein-export membrane protein SecD (TC 3.A.5.1.1), at 92,576 to 94,390
GFF4307
Position (kb)
91
92
93 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 90.225 kb on + strand, within GFF4304 at 90.226 kb on - strand, within GFF4304 at 90.589 kb on + strand, within GFF4304 at 90.624 kb on + strand, within GFF4304 at 90.633 kb on + strand, within GFF4304 at 90.741 kb on + strand at 90.743 kb on + strand at 90.744 kb on - strand at 90.744 kb on - strand at 90.744 kb on - strand at 90.754 kb on + strand at 90.776 kb on + strand at 90.804 kb on + strand at 90.804 kb on + strand at 90.805 kb on - strand at 90.891 kb on - strand at 90.967 kb on + strand at 90.967 kb on + strand at 91.031 kb on + strand at 91.046 kb on + strand at 91.119 kb on + strand at 91.135 kb on + strand at 91.136 kb on - strand at 91.493 kb on - strand, within GFF4305 at 91.498 kb on - strand, within GFF4305 at 91.701 kb on + strand, within GFF4305 at 91.815 kb on + strand, within GFF4305 at 91.815 kb on + strand, within GFF4305 at 91.905 kb on + strand, within GFF4305 at 92.167 kb on + strand at 92.475 kb on + strand, within GFF4306
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 90,225 + GFF4304 0.43 -2.5 90,226 - GFF4304 0.43 -1.3 90,589 + GFF4304 0.77 -0.2 90,624 + GFF4304 0.80 -2.9 90,633 + GFF4304 0.81 -0.5 90,741 + -0.2 90,743 + +0.4 90,744 - -0.4 90,744 - +0.1 90,744 - +0.8 90,754 + -2.1 90,776 + -0.4 90,804 + +1.0 90,804 + +0.0 90,805 - +0.3 90,891 - -1.7 90,967 + +0.1 90,967 + -0.5 91,031 + -2.2 91,046 + +0.6 91,119 + -1.6 91,135 + +0.2 91,136 - -1.3 91,493 - GFF4305 0.41 -1.0 91,498 - GFF4305 0.41 -2.6 91,701 + GFF4305 0.59 -0.5 91,815 + GFF4305 0.69 -1.0 91,815 + GFF4305 0.69 -1.5 91,905 + GFF4305 0.77 -2.1 92,167 + -1.6 92,475 + GFF4306 0.88 -0.4
Or see this region's nucleotide sequence