Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3884

Experiment: Ffm_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3883 and GFF3884 overlap by 4 nucleotidesGFF3884 and GFF3885 are separated by 10 nucleotides GFF3883 - Colanic acid biosysnthesis protein WcaK, at 160,914 to 162,194 GFF3883 GFF3884 - Colanic acid biosynthesis glycosyl transferase WcaL, at 162,191 to 163,411 GFF3884 GFF3885 - Colanic acid biosynthesis protein wcaM, at 163,422 to 164,825 GFF3885 Position (kb) 162 163 164Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 161.266 kb on - strand, within GFF3883at 161.368 kb on - strand, within GFF3883at 161.577 kb on + strand, within GFF3883at 161.765 kb on + strand, within GFF3883at 161.818 kb on - strand, within GFF3883at 161.973 kb on + strand, within GFF3883at 161.982 kb on - strand, within GFF3883at 162.007 kb on + strand, within GFF3883at 162.007 kb on + strand, within GFF3883at 162.266 kb on - strandat 162.322 kb on + strand, within GFF3884at 162.322 kb on + strand, within GFF3884at 162.345 kb on - strand, within GFF3884at 162.422 kb on - strand, within GFF3884at 162.557 kb on - strand, within GFF3884at 162.557 kb on - strand, within GFF3884at 162.653 kb on - strand, within GFF3884at 162.658 kb on + strand, within GFF3884at 163.139 kb on - strand, within GFF3884at 163.185 kb on + strand, within GFF3884at 163.189 kb on + strand, within GFF3884at 163.409 kb on + strandat 163.421 kb on + strandat 163.809 kb on - strand, within GFF3885at 163.996 kb on + strand, within GFF3885at 164.075 kb on + strand, within GFF3885at 164.082 kb on - strand, within GFF3885at 164.134 kb on - strand, within GFF3885at 164.146 kb on - strand, within GFF3885at 164.410 kb on - strand, within GFF3885

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Per-strain Table

Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay
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161,266 - GFF3883 0.27 -1.6
161,368 - GFF3883 0.35 -0.2
161,577 + GFF3883 0.52 +1.1
161,765 + GFF3883 0.66 +1.2
161,818 - GFF3883 0.71 -2.2
161,973 + GFF3883 0.83 +1.1
161,982 - GFF3883 0.83 +0.2
162,007 + GFF3883 0.85 -0.8
162,007 + GFF3883 0.85 -0.7
162,266 - -0.9
162,322 + GFF3884 0.11 +0.4
162,322 + GFF3884 0.11 +1.0
162,345 - GFF3884 0.13 +0.2
162,422 - GFF3884 0.19 -0.0
162,557 - GFF3884 0.30 -0.5
162,557 - GFF3884 0.30 -0.2
162,653 - GFF3884 0.38 -1.6
162,658 + GFF3884 0.38 -4.1
163,139 - GFF3884 0.78 -0.4
163,185 + GFF3884 0.81 -0.6
163,189 + GFF3884 0.82 -0.2
163,409 + +0.6
163,421 + +0.5
163,809 - GFF3885 0.28 -0.3
163,996 + GFF3885 0.41 +1.3
164,075 + GFF3885 0.47 +0.1
164,082 - GFF3885 0.47 -0.3
164,134 - GFF3885 0.51 +0.7
164,146 - GFF3885 0.52 -0.9
164,410 - GFF3885 0.70 -4.2

Or see this region's nucleotide sequence