Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3683 and GFF3684 are separated by 27 nucleotides GFF3684 and GFF3685 are separated by 61 nucleotides GFF3685 and GFF3686 are separated by 184 nucleotides
GFF3683 - FIG001587: exported protein, at 38,721 to 39,269
GFF3683
GFF3684 - Hypothetical metal-binding enzyme, YcbL homolog, at 39,297 to 39,944
GFF3684
GFF3685 - Aspartate aminotransferase (EC 2.6.1.1), at 40,006 to 41,196
GFF3685
GFF3686 - Outer membrane protein F precursor, at 41,381 to 42,472
GFF3686
Position (kb) 40
41
42 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 39.079 kb on + strand, within GFF3683 at 39.090 kb on - strand, within GFF3683 at 39.119 kb on + strand, within GFF3683 at 39.119 kb on + strand, within GFF3683 at 39.146 kb on + strand, within GFF3683 at 39.191 kb on + strand, within GFF3683 at 39.225 kb on - strand at 39.225 kb on - strand at 39.227 kb on + strand at 39.303 kb on + strand at 39.309 kb on - strand at 39.312 kb on - strand at 39.676 kb on + strand, within GFF3684 at 39.676 kb on + strand, within GFF3684 at 39.676 kb on + strand, within GFF3684 at 39.676 kb on + strand, within GFF3684 at 39.884 kb on + strand at 40.215 kb on + strand, within GFF3685 at 40.350 kb on - strand, within GFF3685 at 40.353 kb on + strand, within GFF3685 at 40.444 kb on - strand, within GFF3685 at 40.466 kb on + strand, within GFF3685 at 40.467 kb on - strand, within GFF3685 at 40.483 kb on - strand, within GFF3685 at 40.491 kb on + strand, within GFF3685 at 40.650 kb on + strand, within GFF3685 at 40.751 kb on + strand, within GFF3685 at 40.751 kb on + strand, within GFF3685 at 40.920 kb on + strand, within GFF3685 at 40.921 kb on - strand, within GFF3685 at 41.093 kb on - strand at 41.093 kb on - strand at 41.213 kb on - strand at 41.213 kb on - strand at 41.216 kb on + strand at 41.360 kb on + strand at 41.391 kb on + strand at 41.392 kb on - strand at 41.431 kb on - strand at 41.529 kb on + strand, within GFF3686 at 41.529 kb on + strand, within GFF3686 at 41.626 kb on - strand, within GFF3686 at 41.722 kb on - strand, within GFF3686 at 41.736 kb on + strand, within GFF3686 at 41.736 kb on + strand, within GFF3686 at 41.754 kb on + strand, within GFF3686 at 41.754 kb on + strand, within GFF3686 at 41.838 kb on + strand, within GFF3686 at 41.838 kb on + strand, within GFF3686 at 41.911 kb on + strand, within GFF3686 at 41.911 kb on + strand, within GFF3686 at 41.912 kb on - strand, within GFF3686 at 41.997 kb on + strand, within GFF3686 at 42.031 kb on - strand, within GFF3686 at 42.031 kb on - strand, within GFF3686 at 42.031 kb on - strand, within GFF3686 at 42.090 kb on + strand, within GFF3686
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 39,079 + GFF3683 0.65 +0.2 39,090 - GFF3683 0.67 +0.5 39,119 + GFF3683 0.72 -2.1 39,119 + GFF3683 0.72 +1.2 39,146 + GFF3683 0.77 -0.0 39,191 + GFF3683 0.86 -1.4 39,225 - -2.2 39,225 - -0.7 39,227 + -0.3 39,303 + -3.1 39,309 - -1.7 39,312 - -2.1 39,676 + GFF3684 0.58 -1.1 39,676 + GFF3684 0.58 +0.3 39,676 + GFF3684 0.58 -1.5 39,676 + GFF3684 0.58 +0.1 39,884 + -0.9 40,215 + GFF3685 0.18 -0.4 40,350 - GFF3685 0.29 -2.0 40,353 + GFF3685 0.29 -3.1 40,444 - GFF3685 0.37 -0.3 40,466 + GFF3685 0.39 -0.9 40,467 - GFF3685 0.39 -1.6 40,483 - GFF3685 0.40 -3.9 40,491 + GFF3685 0.41 -0.3 40,650 + GFF3685 0.54 -0.6 40,751 + GFF3685 0.63 -0.9 40,751 + GFF3685 0.63 -0.8 40,920 + GFF3685 0.77 -2.2 40,921 - GFF3685 0.77 -2.8 41,093 - -0.5 41,093 - -0.3 41,213 - -0.7 41,213 - -1.5 41,216 + -1.1 41,360 + +0.6 41,391 + -0.6 41,392 - -0.3 41,431 - +0.7 41,529 + GFF3686 0.14 -1.1 41,529 + GFF3686 0.14 -0.5 41,626 - GFF3686 0.22 -0.0 41,722 - GFF3686 0.31 +0.2 41,736 + GFF3686 0.33 -3.8 41,736 + GFF3686 0.33 -3.0 41,754 + GFF3686 0.34 -2.0 41,754 + GFF3686 0.34 -0.5 41,838 + GFF3686 0.42 -2.6 41,838 + GFF3686 0.42 -0.3 41,911 + GFF3686 0.49 -0.7 41,911 + GFF3686 0.49 -1.0 41,912 - GFF3686 0.49 +3.2 41,997 + GFF3686 0.56 -0.9 42,031 - GFF3686 0.60 +0.8 42,031 - GFF3686 0.60 -2.8 42,031 - GFF3686 0.60 +0.5 42,090 + GFF3686 0.65 -3.0
Or see this region's nucleotide sequence