Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3554 and GFF3555 overlap by 4 nucleotides GFF3555 and GFF3556 are separated by 98 nucleotides GFF3556 and GFF3557 are separated by 287 nucleotides
GFF3554 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 308,557 to 311,613
GFF3554
GFF3555 - 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (EC 3.1.2.28) in menaquinone biosynthesis, at 311,610 to 312,020
GFF3555
GFF3556 - FIG00639040: hypothetical protein, at 312,119 to 312,307
GFF3556
GFF3557 - L-cystine uptake protein TcyP, at 312,595 to 313,941
GFF3557
Position (kb) 311
312
313 Strain fitness (log2 ratio) -2
-1
0
1
2 at 310.811 kb on + strand, within GFF3554 at 310.869 kb on + strand, within GFF3554 at 311.053 kb on - strand, within GFF3554 at 311.053 kb on - strand, within GFF3554 at 311.055 kb on + strand, within GFF3554 at 311.474 kb on + strand at 311.474 kb on + strand at 311.475 kb on - strand at 311.604 kb on + strand at 311.607 kb on - strand at 311.607 kb on - strand at 311.821 kb on - strand, within GFF3555 at 311.956 kb on + strand, within GFF3555 at 312.032 kb on + strand at 312.047 kb on + strand at 312.048 kb on - strand at 312.084 kb on + strand at 312.085 kb on - strand at 312.086 kb on + strand at 312.087 kb on - strand at 312.106 kb on - strand at 312.106 kb on - strand at 312.118 kb on + strand at 312.118 kb on + strand at 312.119 kb on - strand at 312.119 kb on - strand at 312.308 kb on + strand at 312.309 kb on - strand at 312.309 kb on - strand at 312.550 kb on + strand at 312.709 kb on - strand at 312.717 kb on + strand at 312.738 kb on + strand, within GFF3557 at 312.795 kb on + strand, within GFF3557
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 310,811 + GFF3554 0.74 +0.5 310,869 + GFF3554 0.76 -0.7 311,053 - GFF3554 0.82 +0.7 311,053 - GFF3554 0.82 +0.6 311,055 + GFF3554 0.82 -0.0 311,474 + +0.7 311,474 + -2.2 311,475 - -0.0 311,604 + -0.1 311,607 - +0.6 311,607 - -0.3 311,821 - GFF3555 0.51 -1.6 311,956 + GFF3555 0.84 -1.4 312,032 + +1.8 312,047 + +0.8 312,048 - +0.3 312,084 + +0.9 312,085 - -0.2 312,086 + +0.4 312,087 - -0.4 312,106 - +1.9 312,106 - +0.7 312,118 + +0.6 312,118 + +0.2 312,119 - -1.9 312,119 - -1.5 312,308 + +1.5 312,309 - +0.6 312,309 - -1.0 312,550 + -0.4 312,709 - -0.4 312,717 + +0.0 312,738 + GFF3557 0.11 +0.2 312,795 + GFF3557 0.15 -1.1
Or see this region's nucleotide sequence