Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3551 and GFF3552 are separated by 140 nucleotides GFF3552 and GFF3553 are separated by 457 nucleotides GFF3553 and GFF3554 are separated by 264 nucleotides
GFF3551 - Putative transport system permease protein, at 305,005 to 306,216
GFF3551
GFF3552 - Putative cytoplasmic protein, at 306,357 to 306,716
GFF3552
GFF3553 - Putative membrane protein, at 307,174 to 308,292
GFF3553
GFF3554 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 308,557 to 311,613
GFF3554
Position (kb) 307
308
309 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 306.249 kb on - strand at 306.748 kb on + strand at 306.860 kb on - strand at 307.259 kb on - strand at 307.259 kb on - strand at 307.309 kb on - strand, within GFF3553 at 307.485 kb on - strand, within GFF3553 at 307.494 kb on + strand, within GFF3553 at 307.622 kb on - strand, within GFF3553 at 307.730 kb on - strand, within GFF3553 at 307.731 kb on + strand, within GFF3553 at 307.732 kb on - strand, within GFF3553 at 307.857 kb on + strand, within GFF3553 at 307.859 kb on + strand, within GFF3553 at 307.860 kb on - strand, within GFF3553 at 307.882 kb on - strand, within GFF3553 at 307.909 kb on - strand, within GFF3553 at 307.950 kb on + strand, within GFF3553 at 307.951 kb on - strand, within GFF3553 at 308.190 kb on + strand at 308.200 kb on + strand at 308.265 kb on + strand at 308.266 kb on - strand at 308.406 kb on + strand at 308.440 kb on - strand at 308.533 kb on - strand at 308.533 kb on - strand at 308.553 kb on + strand at 308.659 kb on + strand at 308.660 kb on - strand at 308.841 kb on - strand at 308.872 kb on + strand, within GFF3554 at 308.875 kb on - strand, within GFF3554 at 308.936 kb on + strand, within GFF3554 at 309.034 kb on - strand, within GFF3554 at 309.196 kb on + strand, within GFF3554 at 309.197 kb on - strand, within GFF3554 at 309.281 kb on - strand, within GFF3554 at 309.287 kb on + strand, within GFF3554
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 306,249 - -2.7 306,748 + -0.1 306,860 - -1.5 307,259 - -0.4 307,259 - +0.8 307,309 - GFF3553 0.12 +1.2 307,485 - GFF3553 0.28 -1.8 307,494 + GFF3553 0.29 +1.4 307,622 - GFF3553 0.40 +1.1 307,730 - GFF3553 0.50 +1.8 307,731 + GFF3553 0.50 -3.4 307,732 - GFF3553 0.50 +1.7 307,857 + GFF3553 0.61 -1.2 307,859 + GFF3553 0.61 -0.6 307,860 - GFF3553 0.61 +1.1 307,882 - GFF3553 0.63 +1.4 307,909 - GFF3553 0.66 +2.0 307,950 + GFF3553 0.69 -1.2 307,951 - GFF3553 0.69 +0.5 308,190 + -0.1 308,200 + -0.1 308,265 + -1.7 308,266 - -1.0 308,406 + +0.4 308,440 - -0.7 308,533 - -0.2 308,533 - -0.2 308,553 + +0.7 308,659 + -1.0 308,660 - -3.5 308,841 - -0.4 308,872 + GFF3554 0.10 +0.9 308,875 - GFF3554 0.10 -1.2 308,936 + GFF3554 0.12 +0.2 309,034 - GFF3554 0.16 -0.0 309,196 + GFF3554 0.21 -0.1 309,197 - GFF3554 0.21 +0.6 309,281 - GFF3554 0.24 +0.1 309,287 + GFF3554 0.24 +0.2
Or see this region's nucleotide sequence