Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3440 and GFF3441 overlap by 1 nucleotides GFF3441 and GFF3442 overlap by 11 nucleotides GFF3442 and GFF3443 overlap by 7 nucleotides
GFF3440 - Nickel ABC transporter, periplasmic nickel-binding protein nikA2 (TC 3.A.1.5.3), at 201,175 to 202,809
GFF3440
GFF3441 - Nickel transport system permease protein nikB2 (TC 3.A.1.5.3), at 202,809 to 203,783
GFF3441
GFF3442 - Nickel transport system permease protein nikC2 (TC 3.A.1.5.3), at 203,773 to 204,585
GFF3442
GFF3443 - Nickel transport ATP-binding protein nikD2 (TC 3.A.1.5.3), at 204,579 to 205,376
GFF3443
Position (kb) 202
203
204 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 201.811 kb on - strand, within GFF3440 at 201.933 kb on + strand, within GFF3440 at 201.934 kb on - strand, within GFF3440 at 201.934 kb on - strand, within GFF3440 at 201.963 kb on + strand, within GFF3440 at 202.114 kb on - strand, within GFF3440 at 202.159 kb on + strand, within GFF3440 at 202.162 kb on - strand, within GFF3440 at 202.499 kb on - strand, within GFF3440 at 202.528 kb on + strand, within GFF3440 at 202.528 kb on + strand, within GFF3440 at 202.583 kb on + strand, within GFF3440 at 202.583 kb on + strand, within GFF3440 at 202.584 kb on - strand, within GFF3440 at 202.702 kb on - strand at 202.705 kb on + strand at 202.705 kb on + strand at 202.886 kb on + strand at 202.920 kb on - strand, within GFF3441 at 203.227 kb on + strand, within GFF3441 at 203.228 kb on - strand, within GFF3441 at 203.297 kb on - strand, within GFF3441 at 203.384 kb on - strand, within GFF3441 at 203.392 kb on + strand, within GFF3441 at 203.393 kb on - strand, within GFF3441 at 203.510 kb on - strand, within GFF3441 at 203.524 kb on + strand, within GFF3441 at 203.524 kb on + strand, within GFF3441 at 203.524 kb on + strand, within GFF3441 at 203.524 kb on + strand, within GFF3441 at 203.525 kb on - strand, within GFF3441 at 203.554 kb on + strand, within GFF3441 at 203.689 kb on + strand at 203.701 kb on + strand at 203.701 kb on + strand at 203.702 kb on - strand at 203.705 kb on - strand at 203.705 kb on - strand at 203.821 kb on - strand at 203.951 kb on + strand, within GFF3442 at 204.293 kb on - strand, within GFF3442 at 204.491 kb on + strand, within GFF3442 at 204.503 kb on + strand, within GFF3442 at 204.655 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 201,811 - GFF3440 0.39 -0.1 201,933 + GFF3440 0.46 -0.2 201,934 - GFF3440 0.46 -0.4 201,934 - GFF3440 0.46 +0.1 201,963 + GFF3440 0.48 -4.1 202,114 - GFF3440 0.57 +1.2 202,159 + GFF3440 0.60 +0.5 202,162 - GFF3440 0.60 +0.0 202,499 - GFF3440 0.81 -1.8 202,528 + GFF3440 0.83 -0.0 202,528 + GFF3440 0.83 +1.1 202,583 + GFF3440 0.86 +0.3 202,583 + GFF3440 0.86 +0.2 202,584 - GFF3440 0.86 +0.3 202,702 - -1.0 202,705 + +0.1 202,705 + +0.4 202,886 + +3.4 202,920 - GFF3441 0.11 -0.0 203,227 + GFF3441 0.43 +0.9 203,228 - GFF3441 0.43 +1.5 203,297 - GFF3441 0.50 +0.9 203,384 - GFF3441 0.59 -0.6 203,392 + GFF3441 0.60 +0.0 203,393 - GFF3441 0.60 +0.1 203,510 - GFF3441 0.72 +0.6 203,524 + GFF3441 0.73 +0.3 203,524 + GFF3441 0.73 +1.5 203,524 + GFF3441 0.73 +0.6 203,524 + GFF3441 0.73 +0.7 203,525 - GFF3441 0.73 +0.5 203,554 + GFF3441 0.76 +0.6 203,689 + -0.0 203,701 + +0.1 203,701 + -1.0 203,702 - -0.3 203,705 - +0.4 203,705 - +0.9 203,821 - +1.8 203,951 + GFF3442 0.22 +1.6 204,293 - GFF3442 0.64 +0.6 204,491 + GFF3442 0.88 +1.4 204,503 + GFF3442 0.90 +0.9 204,655 + +0.5
Or see this region's nucleotide sequence