Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3401 and GFF3402 are separated by 28 nucleotides GFF3402 and GFF3403 are separated by 18 nucleotides GFF3403 and GFF3404 are separated by 81 nucleotides
GFF3401 - Lipoprotein releasing system transmembrane protein LolE, at 166,624 to 167,868
GFF3401
GFF3402 - N-acetyl-D-glucosamine kinase (EC 2.7.1.59), at 167,897 to 168,808
GFF3402
GFF3403 - NAD-dependent protein deacetylase of SIR2 family, at 168,827 to 169,648
GFF3403
GFF3404 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 169,730 to 170,776
GFF3404
Position (kb) 167
168
169 Strain fitness (log2 ratio) -2
-1
0
1
2 at 167.900 kb on + strand at 167.901 kb on - strand at 168.003 kb on - strand, within GFF3402 at 168.344 kb on + strand, within GFF3402 at 168.345 kb on - strand, within GFF3402 at 168.346 kb on + strand, within GFF3402 at 168.347 kb on - strand, within GFF3402 at 168.367 kb on + strand, within GFF3402 at 168.367 kb on + strand, within GFF3402 at 168.486 kb on + strand, within GFF3402 at 168.486 kb on + strand, within GFF3402 at 168.497 kb on + strand, within GFF3402 at 168.954 kb on + strand, within GFF3403 at 169.102 kb on + strand, within GFF3403 at 169.102 kb on + strand, within GFF3403 at 169.456 kb on + strand, within GFF3403 at 169.531 kb on + strand, within GFF3403 at 169.627 kb on + strand at 169.646 kb on + strand at 169.646 kb on + strand at 169.731 kb on + strand at 169.758 kb on + strand at 169.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 167,900 + -0.1 167,901 - -0.3 168,003 - GFF3402 0.12 -0.3 168,344 + GFF3402 0.49 +0.6 168,345 - GFF3402 0.49 -0.0 168,346 + GFF3402 0.49 +0.1 168,347 - GFF3402 0.49 -1.8 168,367 + GFF3402 0.52 +1.9 168,367 + GFF3402 0.52 +1.2 168,486 + GFF3402 0.65 +0.2 168,486 + GFF3402 0.65 +0.9 168,497 + GFF3402 0.66 +0.3 168,954 + GFF3403 0.15 -0.1 169,102 + GFF3403 0.33 +0.5 169,102 + GFF3403 0.33 +0.3 169,456 + GFF3403 0.77 +0.0 169,531 + GFF3403 0.86 +1.7 169,627 + +0.7 169,646 + +1.7 169,646 + -0.5 169,731 + -0.6 169,758 + +0.5 169,758 + -2.0
Or see this region's nucleotide sequence