Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3326 and GFF3327 are separated by 16 nucleotides GFF3327 and GFF3328 are separated by 253 nucleotides
GFF3326 - Cardiolipin synthetase (EC 2.7.8.-), at 95,722 to 97,026
GFF3326
GFF3327 - Glucans biosynthesis protein C (EC 2.1.-.-), at 97,043 to 98,197
GFF3327
GFF3328 - Glucans biosynthesis protein G precursor, at 98,451 to 100,004
GFF3328
Position (kb) 97
98
99 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 96.188 kb on + strand, within GFF3326 at 96.188 kb on + strand, within GFF3326 at 96.188 kb on + strand, within GFF3326 at 96.189 kb on - strand, within GFF3326 at 96.189 kb on - strand, within GFF3326 at 96.245 kb on - strand, within GFF3326 at 96.246 kb on + strand, within GFF3326 at 96.276 kb on + strand, within GFF3326 at 96.277 kb on - strand, within GFF3326 at 96.369 kb on + strand, within GFF3326 at 96.447 kb on + strand, within GFF3326 at 96.505 kb on - strand, within GFF3326 at 96.539 kb on + strand, within GFF3326 at 96.540 kb on - strand, within GFF3326 at 96.544 kb on - strand, within GFF3326 at 96.668 kb on + strand, within GFF3326 at 96.669 kb on - strand, within GFF3326 at 96.708 kb on - strand, within GFF3326 at 96.714 kb on + strand, within GFF3326 at 96.736 kb on - strand, within GFF3326 at 96.907 kb on - strand at 97.119 kb on - strand at 97.119 kb on - strand at 97.160 kb on + strand, within GFF3327 at 97.252 kb on - strand, within GFF3327 at 97.408 kb on - strand, within GFF3327 at 97.426 kb on - strand, within GFF3327 at 97.599 kb on - strand, within GFF3327 at 97.630 kb on - strand, within GFF3327 at 97.726 kb on + strand, within GFF3327 at 97.727 kb on - strand, within GFF3327 at 98.104 kb on - strand at 98.132 kb on + strand at 98.155 kb on + strand at 98.197 kb on + strand at 98.199 kb on + strand at 98.200 kb on - strand at 98.209 kb on + strand at 98.270 kb on - strand at 98.418 kb on - strand at 98.443 kb on + strand at 98.444 kb on - strand at 98.444 kb on - strand at 98.448 kb on - strand at 98.459 kb on + strand at 98.459 kb on + strand at 98.502 kb on - strand at 98.689 kb on + strand, within GFF3328 at 98.689 kb on + strand, within GFF3328 at 98.690 kb on - strand, within GFF3328 at 98.703 kb on - strand, within GFF3328 at 98.730 kb on + strand, within GFF3328 at 98.803 kb on - strand, within GFF3328 at 98.918 kb on + strand, within GFF3328 at 98.934 kb on + strand, within GFF3328 at 98.934 kb on + strand, within GFF3328 at 98.965 kb on - strand, within GFF3328 at 98.972 kb on - strand, within GFF3328
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 96,188 + GFF3326 0.36 +0.7 96,188 + GFF3326 0.36 +0.5 96,188 + GFF3326 0.36 +2.3 96,189 - GFF3326 0.36 +0.6 96,189 - GFF3326 0.36 -1.5 96,245 - GFF3326 0.40 +0.5 96,246 + GFF3326 0.40 +0.2 96,276 + GFF3326 0.42 +0.2 96,277 - GFF3326 0.43 +0.0 96,369 + GFF3326 0.50 -3.3 96,447 + GFF3326 0.56 -1.0 96,505 - GFF3326 0.60 +0.4 96,539 + GFF3326 0.63 -0.3 96,540 - GFF3326 0.63 -0.8 96,544 - GFF3326 0.63 -1.3 96,668 + GFF3326 0.72 +0.3 96,669 - GFF3326 0.73 +0.5 96,708 - GFF3326 0.76 +0.7 96,714 + GFF3326 0.76 +0.5 96,736 - GFF3326 0.78 +0.1 96,907 - +0.8 97,119 - -0.9 97,119 - -2.6 97,160 + GFF3327 0.10 -1.2 97,252 - GFF3327 0.18 -0.5 97,408 - GFF3327 0.32 +0.8 97,426 - GFF3327 0.33 -0.3 97,599 - GFF3327 0.48 -1.3 97,630 - GFF3327 0.51 +0.4 97,726 + GFF3327 0.59 -3.7 97,727 - GFF3327 0.59 +0.1 98,104 - +0.2 98,132 + -0.5 98,155 + -0.2 98,197 + -2.6 98,199 + +0.8 98,200 - +0.4 98,209 + +1.5 98,270 - -1.1 98,418 - -0.1 98,443 + -0.9 98,444 - -0.8 98,444 - +0.6 98,448 - +0.4 98,459 + -1.9 98,459 + +1.1 98,502 - +0.6 98,689 + GFF3328 0.15 -2.8 98,689 + GFF3328 0.15 +0.5 98,690 - GFF3328 0.15 -0.3 98,703 - GFF3328 0.16 -0.1 98,730 + GFF3328 0.18 -1.6 98,803 - GFF3328 0.23 -0.5 98,918 + GFF3328 0.30 +0.1 98,934 + GFF3328 0.31 -0.5 98,934 + GFF3328 0.31 +0.2 98,965 - GFF3328 0.33 +0.2 98,972 - GFF3328 0.34 -0.2
Or see this region's nucleotide sequence