Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF328 and GFF329 overlap by 14 nucleotides GFF329 and GFF330 are separated by 235 nucleotides
GFF328 - L-seryl-tRNA(Sec) selenium transferase-related protein, at 43,425 to 44,534
GFF328
GFF329 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16), at 44,521 to 45,264
GFF329
GFF330 - Transposase, at 45,500 to 46,441
GFF330
Position (kb) 44
45
46 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 43.607 kb on + strand, within GFF328 at 43.622 kb on + strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.623 kb on - strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.626 kb on + strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.627 kb on - strand, within GFF328 at 43.742 kb on - strand, within GFF328 at 43.760 kb on + strand, within GFF328 at 44.045 kb on + strand, within GFF328 at 44.049 kb on + strand, within GFF328 at 44.050 kb on - strand, within GFF328 at 44.050 kb on - strand, within GFF328 at 44.070 kb on - strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.099 kb on + strand, within GFF328 at 44.190 kb on + strand, within GFF328 at 44.191 kb on - strand, within GFF328 at 44.600 kb on - strand, within GFF329 at 44.600 kb on - strand, within GFF329 at 44.600 kb on - strand, within GFF329 at 44.600 kb on - strand, within GFF329 at 44.600 kb on - strand, within GFF329 at 44.657 kb on + strand, within GFF329 at 44.657 kb on + strand, within GFF329 at 44.743 kb on + strand, within GFF329 at 44.743 kb on + strand, within GFF329 at 44.746 kb on + strand, within GFF329 at 44.773 kb on - strand, within GFF329 at 45.041 kb on + strand, within GFF329 at 45.041 kb on + strand, within GFF329 at 45.041 kb on + strand, within GFF329 at 45.042 kb on - strand, within GFF329 at 45.245 kb on + strand at 45.246 kb on - strand at 45.313 kb on + strand at 45.314 kb on - strand at 45.314 kb on - strand at 45.382 kb on - strand at 45.382 kb on - strand at 45.606 kb on + strand, within GFF330 at 45.725 kb on + strand, within GFF330 at 45.728 kb on - strand, within GFF330 at 45.728 kb on - strand, within GFF330 at 45.728 kb on - strand, within GFF330 at 45.731 kb on + strand, within GFF330 at 45.731 kb on + strand, within GFF330 at 45.732 kb on - strand, within GFF330 at 45.739 kb on + strand, within GFF330 at 45.740 kb on - strand, within GFF330 at 45.833 kb on - strand, within GFF330 at 45.866 kb on + strand, within GFF330 at 45.970 kb on - strand, within GFF330 at 45.970 kb on - strand, within GFF330 at 46.046 kb on - strand, within GFF330 at 46.046 kb on - strand, within GFF330 at 46.047 kb on + strand, within GFF330 at 46.048 kb on - strand, within GFF330 at 46.048 kb on - strand, within GFF330 at 46.251 kb on + strand, within GFF330 at 46.251 kb on + strand, within GFF330 at 46.251 kb on + strand, within GFF330
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 43,607 + GFF328 0.16 +0.9 43,622 + GFF328 0.18 +0.7 43,623 - GFF328 0.18 -0.6 43,623 - GFF328 0.18 -1.0 43,623 - GFF328 0.18 +0.7 43,626 + GFF328 0.18 +2.1 43,626 + GFF328 0.18 -2.0 43,626 + GFF328 0.18 -1.7 43,626 + GFF328 0.18 -2.6 43,626 + GFF328 0.18 +0.6 43,626 + GFF328 0.18 +0.0 43,626 + GFF328 0.18 -0.6 43,626 + GFF328 0.18 +0.9 43,627 - GFF328 0.18 +0.2 43,627 - GFF328 0.18 +2.3 43,627 - GFF328 0.18 +0.6 43,627 - GFF328 0.18 +0.5 43,742 - GFF328 0.29 +1.0 43,760 + GFF328 0.30 +0.5 44,045 + GFF328 0.56 -1.9 44,049 + GFF328 0.56 -0.1 44,050 - GFF328 0.56 -0.2 44,050 - GFF328 0.56 +1.0 44,070 - GFF328 0.58 -0.1 44,099 + GFF328 0.61 -1.2 44,099 + GFF328 0.61 +0.1 44,099 + GFF328 0.61 -0.2 44,190 + GFF328 0.69 -0.3 44,191 - GFF328 0.69 -1.2 44,600 - GFF329 0.11 -2.4 44,600 - GFF329 0.11 +0.4 44,600 - GFF329 0.11 +0.7 44,600 - GFF329 0.11 -0.4 44,600 - GFF329 0.11 +0.7 44,657 + GFF329 0.18 +0.2 44,657 + GFF329 0.18 -1.1 44,743 + GFF329 0.30 -0.8 44,743 + GFF329 0.30 -0.0 44,746 + GFF329 0.30 -1.2 44,773 - GFF329 0.34 -1.1 45,041 + GFF329 0.70 -0.2 45,041 + GFF329 0.70 +1.4 45,041 + GFF329 0.70 +0.0 45,042 - GFF329 0.70 -0.6 45,245 + +0.4 45,246 - +0.5 45,313 + +0.7 45,314 - -2.3 45,314 - -4.3 45,382 - -0.2 45,382 - -2.3 45,606 + GFF330 0.11 -0.3 45,725 + GFF330 0.24 +0.2 45,728 - GFF330 0.24 +0.1 45,728 - GFF330 0.24 -0.2 45,728 - GFF330 0.24 -0.4 45,731 + GFF330 0.25 -1.5 45,731 + GFF330 0.25 +0.3 45,732 - GFF330 0.25 -0.5 45,739 + GFF330 0.25 +2.2 45,740 - GFF330 0.25 +2.5 45,833 - GFF330 0.35 -0.6 45,866 + GFF330 0.39 -0.4 45,970 - GFF330 0.50 -0.8 45,970 - GFF330 0.50 -0.1 46,046 - GFF330 0.58 -0.1 46,046 - GFF330 0.58 -0.9 46,047 + GFF330 0.58 +0.4 46,048 - GFF330 0.58 -2.8 46,048 - GFF330 0.58 -0.2 46,251 + GFF330 0.80 +1.0 46,251 + GFF330 0.80 -0.4 46,251 + GFF330 0.80 +4.0
Or see this region's nucleotide sequence