Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3242 and GFF3243 overlap by 4 nucleotides GFF3243 and GFF3244 overlap by 46 nucleotides GFF3244 and GFF3245 are separated by 430 nucleotides
GFF3242 - Paraquat-inducible protein B, at 17,051 to 18,691
GFF3242
GFF3243 - Paraquat-inducible protein B, at 18,688 to 19,251
GFF3243
GFF3244 - hypothetical protein, at 19,206 to 19,343
GFF3244
GFF3245 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, FabA form (EC 4.2.1.59), at 19,774 to 20,292
GFF3245
Position (kb) 19
20 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 18.260 kb on + strand, within GFF3242 at 18.398 kb on - strand, within GFF3242 at 18.398 kb on - strand, within GFF3242 at 18.430 kb on + strand, within GFF3242 at 18.430 kb on + strand, within GFF3242 at 18.431 kb on - strand, within GFF3242 at 18.442 kb on + strand, within GFF3242 at 18.542 kb on + strand at 18.543 kb on - strand at 18.656 kb on - strand at 18.760 kb on + strand, within GFF3243 at 18.763 kb on + strand, within GFF3243 at 18.824 kb on + strand, within GFF3243 at 18.824 kb on + strand, within GFF3243 at 18.845 kb on + strand, within GFF3243 at 18.853 kb on + strand, within GFF3243 at 18.898 kb on + strand, within GFF3243 at 18.899 kb on - strand, within GFF3243 at 19.168 kb on + strand, within GFF3243 at 19.261 kb on - strand, within GFF3244 at 19.261 kb on - strand, within GFF3244 at 19.368 kb on + strand at 19.430 kb on + strand at 19.490 kb on + strand at 19.555 kb on + strand at 19.761 kb on - strand at 20.331 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 18,260 + GFF3242 0.74 +1.3 18,398 - GFF3242 0.82 -3.0 18,398 - GFF3242 0.82 -1.4 18,430 + GFF3242 0.84 +0.9 18,430 + GFF3242 0.84 -3.3 18,431 - GFF3242 0.84 -1.2 18,442 + GFF3242 0.85 -0.2 18,542 + +0.4 18,543 - -2.0 18,656 - +0.4 18,760 + GFF3243 0.13 +0.9 18,763 + GFF3243 0.13 +2.0 18,824 + GFF3243 0.24 -0.1 18,824 + GFF3243 0.24 -0.9 18,845 + GFF3243 0.28 +1.2 18,853 + GFF3243 0.29 +0.0 18,898 + GFF3243 0.37 -0.1 18,899 - GFF3243 0.37 +0.4 19,168 + GFF3243 0.85 -0.8 19,261 - GFF3244 0.40 -3.2 19,261 - GFF3244 0.40 -1.7 19,368 + +1.1 19,430 + +0.8 19,490 + +1.0 19,555 + +0.2 19,761 - +0.0 20,331 - -2.1
Or see this region's nucleotide sequence