Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3195 and GFF3196 are separated by 2 nucleotides GFF3196 and GFF3197 overlap by 4 nucleotides GFF3197 and GFF3198 are separated by 144 nucleotides GFF3198 and GFF3199 are separated by 67 nucleotides
GFF3195 - Uncharacterized membrane protein YqjD, at 201,933 to 202,238
GFF3195
GFF3196 - Inner membrane protein YqjE, at 202,241 to 202,639
GFF3196
GFF3197 - Inner membrane protein YqjK, at 202,636 to 202,935
GFF3197
GFF3198 - Inner membrane protein YqjF, at 203,080 to 203,565
GFF3198
GFF3199 - Glutathione S-transferase, omega (EC 2.5.1.18), at 203,633 to 204,619
GFF3199
Position (kb) 203
204 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 202.155 kb on + strand, within GFF3195 at 202.220 kb on - strand at 202.350 kb on + strand, within GFF3196 at 202.767 kb on + strand, within GFF3197 at 202.775 kb on + strand, within GFF3197 at 202.812 kb on - strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 203.281 kb on + strand, within GFF3198 at 203.281 kb on + strand, within GFF3198 at 203.282 kb on - strand, within GFF3198 at 203.327 kb on + strand, within GFF3198 at 203.406 kb on + strand, within GFF3198 at 203.453 kb on + strand, within GFF3198 at 203.454 kb on - strand, within GFF3198 at 203.504 kb on + strand, within GFF3198 at 203.573 kb on - strand at 203.574 kb on + strand at 203.583 kb on - strand at 203.735 kb on - strand, within GFF3199 at 203.990 kb on + strand, within GFF3199 at 204.062 kb on - strand, within GFF3199 at 204.062 kb on - strand, within GFF3199 at 204.093 kb on + strand, within GFF3199 at 204.095 kb on - strand, within GFF3199 at 204.140 kb on + strand, within GFF3199 at 204.141 kb on - strand, within GFF3199 at 204.262 kb on - strand, within GFF3199 at 204.270 kb on + strand, within GFF3199 at 204.270 kb on + strand, within GFF3199 at 204.318 kb on - strand, within GFF3199 at 204.460 kb on + strand, within GFF3199 at 204.461 kb on - strand, within GFF3199 at 204.471 kb on + strand, within GFF3199 at 204.506 kb on - strand, within GFF3199
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 202,155 + GFF3195 0.73 -0.6 202,220 - -2.6 202,350 + GFF3196 0.27 -0.1 202,767 + GFF3197 0.44 +0.2 202,775 + GFF3197 0.46 -2.3 202,812 - GFF3197 0.59 +1.6 202,842 + GFF3197 0.69 -0.7 202,842 + GFF3197 0.69 +0.3 202,842 + GFF3197 0.69 +0.9 202,843 - GFF3197 0.69 +0.5 202,843 - GFF3197 0.69 +1.2 202,843 - GFF3197 0.69 +1.0 202,843 - GFF3197 0.69 -1.6 203,281 + GFF3198 0.41 +0.4 203,281 + GFF3198 0.41 +0.2 203,282 - GFF3198 0.42 +0.0 203,327 + GFF3198 0.51 -0.6 203,406 + GFF3198 0.67 +0.1 203,453 + GFF3198 0.77 +0.6 203,454 - GFF3198 0.77 -0.3 203,504 + GFF3198 0.87 +0.1 203,573 - -2.6 203,574 + +0.4 203,583 - +0.9 203,735 - GFF3199 0.10 +0.1 203,990 + GFF3199 0.36 +0.9 204,062 - GFF3199 0.43 -3.0 204,062 - GFF3199 0.43 -0.8 204,093 + GFF3199 0.47 -0.9 204,095 - GFF3199 0.47 -2.0 204,140 + GFF3199 0.51 -0.3 204,141 - GFF3199 0.51 +0.1 204,262 - GFF3199 0.64 +0.9 204,270 + GFF3199 0.65 -4.1 204,270 + GFF3199 0.65 -0.8 204,318 - GFF3199 0.69 +1.0 204,460 + GFF3199 0.84 +0.4 204,461 - GFF3199 0.84 +0.2 204,471 + GFF3199 0.85 -0.0 204,506 - GFF3199 0.88 -1.3
Or see this region's nucleotide sequence