Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3143

Experiment: Ffm_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3140 and GFF3141 overlap by 4 nucleotidesGFF3141 and GFF3142 are separated by 31 nucleotidesGFF3142 and GFF3143 are separated by 4 nucleotidesGFF3143 and GFF3144 are separated by 3 nucleotides GFF3140 - hypothetical protein, at 147,932 to 148,048 GFF3140 GFF3141 - Modulator of drug activity B, at 148,045 to 148,626 GFF3141 GFF3142 - FIG00896075: hypothetical protein, at 148,658 to 148,972 GFF3142 GFF3143 - hypothetical protein, at 148,977 to 149,093 GFF3143 GFF3144 - Topoisomerase IV subunit B (EC 5.99.1.-), at 149,097 to 150,989 GFF3144 Position (kb) 148 149 150Strain fitness (log2 ratio) -3 -2 -1 0 1at 148.186 kb on + strand, within GFF3141at 148.288 kb on - strand, within GFF3141at 148.325 kb on - strand, within GFF3141at 148.401 kb on + strand, within GFF3141at 148.470 kb on - strand, within GFF3141at 148.476 kb on - strand, within GFF3141at 148.584 kb on + strandat 148.653 kb on + strandat 148.654 kb on - strandat 148.754 kb on - strand, within GFF3142at 148.898 kb on - strand, within GFF3142at 148.978 kb on + strandat 149.079 kb on + strand, within GFF3143at 149.079 kb on + strand, within GFF3143

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Per-strain Table

Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay
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148,186 + GFF3141 0.24 +0.5
148,288 - GFF3141 0.42 +0.0
148,325 - GFF3141 0.48 -0.4
148,401 + GFF3141 0.61 +0.4
148,470 - GFF3141 0.73 +0.5
148,476 - GFF3141 0.74 +0.2
148,584 + +0.4
148,653 + +0.1
148,654 - +0.1
148,754 - GFF3142 0.30 +0.3
148,898 - GFF3142 0.76 -0.4
148,978 + +1.2
149,079 + GFF3143 0.87 -2.0
149,079 + GFF3143 0.87 -3.0

Or see this region's nucleotide sequence