Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3122 and GFF3123 overlap by 11 nucleotides GFF3123 and GFF3124 are separated by 86 nucleotides GFF3124 and GFF3125 are separated by 105 nucleotides
GFF3122 - Transcriptional regulator, AraC family, at 128,180 to 129,079
GFF3122
GFF3123 - hypothetical protein, at 129,069 to 129,185
GFF3123
GFF3124 - NADPH-dependent broad range aldehyde dehydrogenase YqhD, at 129,272 to 130,435
GFF3124
GFF3125 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconate reductase A (EC 1.1.1.274), at 130,541 to 131,368
GFF3125
Position (kb)
129
130
131 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 128.470 kb on - strand, within GFF3122 at 128.488 kb on + strand, within GFF3122 at 128.850 kb on - strand, within GFF3122 at 128.976 kb on + strand, within GFF3122 at 129.012 kb on - strand at 129.158 kb on + strand, within GFF3123 at 129.158 kb on + strand, within GFF3123 at 129.288 kb on + strand at 129.288 kb on + strand at 129.377 kb on + strand at 129.378 kb on - strand at 129.378 kb on - strand at 129.433 kb on - strand, within GFF3124 at 129.482 kb on + strand, within GFF3124 at 129.527 kb on - strand, within GFF3124 at 129.599 kb on + strand, within GFF3124 at 129.807 kb on - strand, within GFF3124 at 129.814 kb on + strand, within GFF3124 at 129.814 kb on + strand, within GFF3124 at 129.815 kb on - strand, within GFF3124 at 129.815 kb on - strand, within GFF3124 at 129.815 kb on - strand, within GFF3124 at 129.866 kb on - strand, within GFF3124 at 129.878 kb on + strand, within GFF3124 at 129.879 kb on - strand, within GFF3124 at 129.933 kb on + strand, within GFF3124 at 129.984 kb on - strand, within GFF3124 at 130.015 kb on + strand, within GFF3124 at 130.072 kb on + strand, within GFF3124 at 130.072 kb on + strand, within GFF3124 at 130.168 kb on + strand, within GFF3124 at 130.190 kb on + strand, within GFF3124 at 130.190 kb on + strand, within GFF3124 at 130.191 kb on - strand, within GFF3124 at 130.237 kb on + strand, within GFF3124 at 130.238 kb on - strand, within GFF3124 at 130.292 kb on - strand, within GFF3124 at 130.482 kb on + strand at 130.482 kb on + strand at 130.483 kb on - strand at 130.524 kb on + strand at 130.581 kb on + strand at 130.582 kb on - strand at 130.839 kb on - strand, within GFF3125 at 130.839 kb on - strand, within GFF3125 at 130.936 kb on - strand, within GFF3125 at 130.954 kb on + strand, within GFF3125 at 131.055 kb on + strand, within GFF3125 at 131.128 kb on - strand, within GFF3125
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 128,470 - GFF3122 0.32 +0.4 128,488 + GFF3122 0.34 +2.0 128,850 - GFF3122 0.74 +0.1 128,976 + GFF3122 0.88 +0.8 129,012 - -0.1 129,158 + GFF3123 0.76 +0.5 129,158 + GFF3123 0.76 -4.0 129,288 + -2.2 129,288 + -0.9 129,377 + +1.0 129,378 - +0.3 129,378 - -0.8 129,433 - GFF3124 0.14 -0.1 129,482 + GFF3124 0.18 +0.2 129,527 - GFF3124 0.22 +0.1 129,599 + GFF3124 0.28 +0.4 129,807 - GFF3124 0.46 +0.0 129,814 + GFF3124 0.47 +0.3 129,814 + GFF3124 0.47 +0.2 129,815 - GFF3124 0.47 +2.7 129,815 - GFF3124 0.47 -1.1 129,815 - GFF3124 0.47 +0.2 129,866 - GFF3124 0.51 -0.1 129,878 + GFF3124 0.52 +2.0 129,879 - GFF3124 0.52 -0.1 129,933 + GFF3124 0.57 +0.3 129,984 - GFF3124 0.61 +0.6 130,015 + GFF3124 0.64 -1.1 130,072 + GFF3124 0.69 +0.5 130,072 + GFF3124 0.69 +0.1 130,168 + GFF3124 0.77 +0.9 130,190 + GFF3124 0.79 -0.7 130,190 + GFF3124 0.79 -2.5 130,191 - GFF3124 0.79 +1.3 130,237 + GFF3124 0.83 +0.6 130,238 - GFF3124 0.83 -2.1 130,292 - GFF3124 0.88 +1.3 130,482 + +0.3 130,482 + -0.1 130,483 - +0.7 130,524 + -2.1 130,581 + -0.5 130,582 - +1.4 130,839 - GFF3125 0.36 +0.7 130,839 - GFF3125 0.36 -2.6 130,936 - GFF3125 0.48 -0.8 130,954 + GFF3125 0.50 +0.6 131,055 + GFF3125 0.62 +1.4 131,128 - GFF3125 0.71 +0.3
Or see this region's nucleotide sequence