Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3073 and GFF3074 are separated by 24 nucleotides GFF3074 and GFF3075 are separated by 23 nucleotides GFF3075 and GFF3076 are separated by 91 nucleotides
GFF3073 - 4-hydroxybutyrate:acetyl-CoA CoA transferase (EC 2.3.1.-), at 81,960 to 83,285
GFF3073
GFF3074 - Acyl dehydratase, at 83,310 to 83,837
GFF3074
GFF3075 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 83,861 to 84,703
GFF3075
GFF3076 - LysR family transcriptional regulator STM3121, at 84,795 to 85,673
GFF3076
Position (kb) 83
84 Strain fitness (log2 ratio) -1
0
1 at 82.421 kb on - strand, within GFF3073 at 82.421 kb on - strand, within GFF3073 at 82.425 kb on - strand, within GFF3073 at 82.800 kb on - strand, within GFF3073 at 82.871 kb on + strand, within GFF3073 at 82.961 kb on - strand, within GFF3073 at 82.961 kb on - strand, within GFF3073 at 82.961 kb on - strand, within GFF3073 at 83.027 kb on + strand, within GFF3073 at 83.027 kb on + strand, within GFF3073 at 83.199 kb on - strand at 83.223 kb on + strand at 83.264 kb on - strand at 83.356 kb on - strand at 83.403 kb on - strand, within GFF3074 at 83.403 kb on - strand, within GFF3074 at 83.482 kb on + strand, within GFF3074 at 83.489 kb on + strand, within GFF3074 at 83.490 kb on - strand, within GFF3074 at 83.564 kb on + strand, within GFF3074 at 83.922 kb on - strand at 84.023 kb on + strand, within GFF3075 at 84.322 kb on + strand, within GFF3075 at 84.362 kb on - strand, within GFF3075 at 84.418 kb on - strand, within GFF3075 at 84.448 kb on + strand, within GFF3075 at 84.448 kb on + strand, within GFF3075 at 84.449 kb on - strand, within GFF3075 at 84.449 kb on - strand, within GFF3075 at 84.494 kb on + strand, within GFF3075 at 84.674 kb on - strand at 84.674 kb on - strand at 84.757 kb on + strand at 84.758 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 82,421 - GFF3073 0.35 +0.1 82,421 - GFF3073 0.35 +0.6 82,425 - GFF3073 0.35 +0.6 82,800 - GFF3073 0.63 -0.9 82,871 + GFF3073 0.69 +1.1 82,961 - GFF3073 0.75 -0.0 82,961 - GFF3073 0.75 +0.2 82,961 - GFF3073 0.75 +0.9 83,027 + GFF3073 0.80 +1.2 83,027 + GFF3073 0.80 -0.8 83,199 - -0.1 83,223 + -0.9 83,264 - +0.3 83,356 - +0.4 83,403 - GFF3074 0.18 -0.2 83,403 - GFF3074 0.18 +0.4 83,482 + GFF3074 0.33 +0.4 83,489 + GFF3074 0.34 +0.2 83,490 - GFF3074 0.34 -1.5 83,564 + GFF3074 0.48 +0.4 83,922 - +0.6 84,023 + GFF3075 0.19 -0.8 84,322 + GFF3075 0.55 -0.8 84,362 - GFF3075 0.59 +0.1 84,418 - GFF3075 0.66 +0.0 84,448 + GFF3075 0.70 +0.1 84,448 + GFF3075 0.70 +0.3 84,449 - GFF3075 0.70 +0.3 84,449 - GFF3075 0.70 -1.1 84,494 + GFF3075 0.75 +0.1 84,674 - -0.4 84,674 - -0.4 84,757 + -0.3 84,758 - +0.9
Or see this region's nucleotide sequence