Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3069 and GFF3070 are separated by 61 nucleotides GFF3070 and GFF3071 are separated by 272 nucleotides GFF3071 and GFF3072 are separated by 38 nucleotides
GFF3069 - Nucleoside permease NupG, at 76,590 to 77,846
GFF3069
GFF3070 - Ornithine decarboxylase (EC 4.1.1.17), at 77,908 to 80,043
GFF3070
GFF3071 - hypothetical protein, at 80,316 to 80,429
GFF3071
GFF3072 - Putative inner membrane protein YqgA, at 80,468 to 81,175
GFF3072
Position (kb)
77
78
79
80
81 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 76.908 kb on - strand, within GFF3069 at 76.931 kb on + strand, within GFF3069 at 76.932 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.941 kb on + strand, within GFF3069 at 76.970 kb on + strand, within GFF3069 at 77.019 kb on - strand, within GFF3069 at 77.019 kb on - strand, within GFF3069 at 77.076 kb on + strand, within GFF3069 at 77.121 kb on - strand, within GFF3069 at 77.298 kb on - strand, within GFF3069 at 77.298 kb on - strand, within GFF3069 at 77.334 kb on - strand, within GFF3069 at 77.429 kb on + strand, within GFF3069 at 77.444 kb on + strand, within GFF3069 at 77.445 kb on - strand, within GFF3069 at 77.451 kb on - strand, within GFF3069 at 77.600 kb on - strand, within GFF3069 at 77.688 kb on - strand, within GFF3069 at 77.757 kb on + strand at 77.800 kb on - strand at 77.800 kb on - strand at 77.922 kb on - strand at 77.928 kb on - strand at 77.928 kb on - strand at 77.961 kb on + strand at 77.962 kb on - strand at 77.963 kb on + strand at 77.966 kb on - strand at 77.966 kb on - strand at 78.135 kb on + strand, within GFF3070 at 78.148 kb on + strand, within GFF3070 at 78.260 kb on + strand, within GFF3070 at 78.260 kb on + strand, within GFF3070 at 78.283 kb on + strand, within GFF3070 at 78.283 kb on + strand, within GFF3070 at 78.284 kb on - strand, within GFF3070 at 78.500 kb on + strand, within GFF3070 at 78.501 kb on - strand, within GFF3070 at 78.618 kb on - strand, within GFF3070 at 79.005 kb on + strand, within GFF3070 at 79.005 kb on + strand, within GFF3070 at 79.005 kb on + strand, within GFF3070 at 79.006 kb on - strand, within GFF3070 at 79.006 kb on - strand, within GFF3070 at 79.010 kb on - strand, within GFF3070 at 79.153 kb on - strand, within GFF3070 at 79.175 kb on + strand, within GFF3070 at 79.179 kb on + strand, within GFF3070 at 79.180 kb on - strand, within GFF3070 at 79.180 kb on - strand, within GFF3070 at 79.190 kb on + strand, within GFF3070 at 79.191 kb on - strand, within GFF3070 at 79.277 kb on + strand, within GFF3070 at 79.347 kb on - strand, within GFF3070 at 79.491 kb on - strand, within GFF3070 at 79.555 kb on + strand, within GFF3070 at 79.556 kb on - strand, within GFF3070 at 79.559 kb on - strand, within GFF3070 at 79.695 kb on + strand, within GFF3070 at 79.699 kb on - strand, within GFF3070 at 79.810 kb on + strand, within GFF3070 at 79.811 kb on - strand, within GFF3070 at 79.811 kb on - strand, within GFF3070 at 79.822 kb on - strand, within GFF3070 at 79.860 kb on + strand at 79.929 kb on + strand at 79.929 kb on + strand at 80.029 kb on - strand at 80.128 kb on - strand at 80.177 kb on - strand at 80.285 kb on + strand at 80.388 kb on + strand, within GFF3071 at 80.412 kb on - strand, within GFF3071 at 80.485 kb on + strand at 80.505 kb on + strand at 80.734 kb on - strand, within GFF3072 at 80.738 kb on - strand, within GFF3072 at 80.974 kb on - strand, within GFF3072
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 76,908 - GFF3069 0.25 -1.6 76,931 + GFF3069 0.27 +0.5 76,932 - GFF3069 0.27 -0.3 76,935 - GFF3069 0.27 +0.6 76,935 - GFF3069 0.27 -0.0 76,935 - GFF3069 0.27 +0.1 76,941 + GFF3069 0.28 +0.5 76,970 + GFF3069 0.30 -0.2 77,019 - GFF3069 0.34 +1.2 77,019 - GFF3069 0.34 -0.8 77,076 + GFF3069 0.39 -1.0 77,121 - GFF3069 0.42 +0.2 77,298 - GFF3069 0.56 +0.1 77,298 - GFF3069 0.56 -0.3 77,334 - GFF3069 0.59 +0.0 77,429 + GFF3069 0.67 -2.3 77,444 + GFF3069 0.68 -1.9 77,445 - GFF3069 0.68 -2.6 77,451 - GFF3069 0.68 +1.0 77,600 - GFF3069 0.80 -3.7 77,688 - GFF3069 0.87 -0.0 77,757 + -0.7 77,800 - +0.9 77,800 - +0.6 77,922 - -0.7 77,928 - -1.5 77,928 - +0.4 77,961 + -0.6 77,962 - -2.0 77,963 + -2.5 77,966 - +0.5 77,966 - -0.3 78,135 + GFF3070 0.11 +0.8 78,148 + GFF3070 0.11 +2.0 78,260 + GFF3070 0.16 -0.2 78,260 + GFF3070 0.16 -0.4 78,283 + GFF3070 0.18 +1.1 78,283 + GFF3070 0.18 -2.1 78,284 - GFF3070 0.18 -0.3 78,500 + GFF3070 0.28 +1.1 78,501 - GFF3070 0.28 -1.0 78,618 - GFF3070 0.33 +0.4 79,005 + GFF3070 0.51 -0.3 79,005 + GFF3070 0.51 +0.2 79,005 + GFF3070 0.51 +0.3 79,006 - GFF3070 0.51 -0.5 79,006 - GFF3070 0.51 -0.3 79,010 - GFF3070 0.52 -0.0 79,153 - GFF3070 0.58 -2.0 79,175 + GFF3070 0.59 +2.2 79,179 + GFF3070 0.60 +0.4 79,180 - GFF3070 0.60 +1.4 79,180 - GFF3070 0.60 -0.4 79,190 + GFF3070 0.60 +0.3 79,191 - GFF3070 0.60 -0.2 79,277 + GFF3070 0.64 +1.5 79,347 - GFF3070 0.67 -0.3 79,491 - GFF3070 0.74 +3.1 79,555 + GFF3070 0.77 +0.3 79,556 - GFF3070 0.77 +0.6 79,559 - GFF3070 0.77 +0.2 79,695 + GFF3070 0.84 +0.8 79,699 - GFF3070 0.84 +1.4 79,810 + GFF3070 0.89 +0.6 79,811 - GFF3070 0.89 -0.5 79,811 - GFF3070 0.89 +0.3 79,822 - GFF3070 0.90 +0.3 79,860 + +0.4 79,929 + -1.5 79,929 + +0.2 80,029 - +0.6 80,128 - -0.0 80,177 - +1.5 80,285 + -0.8 80,388 + GFF3071 0.63 -0.3 80,412 - GFF3071 0.84 +1.0 80,485 + -1.8 80,505 + +0.4 80,734 - GFF3072 0.38 +0.2 80,738 - GFF3072 0.38 -0.8 80,974 - GFF3072 0.71 +0.2
Or see this region's nucleotide sequence