Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2524 and GFF2525 are separated by 2 nucleotides GFF2525 and GFF2526 are separated by 238 nucleotides GFF2526 and GFF2527 are separated by 148 nucleotides
GFF2524 - Uptake hydrogenase large subunit (EC 1.12.99.6), at 37,613 to 39,415
GFF2524
GFF2525 - Uptake hydrogenase small subunit precursor (EC 1.12.99.6), at 39,418 to 40,410
GFF2525
GFF2526 - hypothetical protein, at 40,649 to 40,801
GFF2526
GFF2527 - Choloylglycine hydrolase (EC 3.5.1.24), at 40,950 to 42,041
GFF2527
Position (kb) 39
40
41 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 38.440 kb on + strand, within GFF2524 at 38.860 kb on - strand, within GFF2524 at 38.860 kb on - strand, within GFF2524 at 39.074 kb on + strand, within GFF2524 at 39.079 kb on + strand, within GFF2524 at 39.091 kb on + strand, within GFF2524 at 39.296 kb on - strand at 39.296 kb on - strand at 39.608 kb on + strand, within GFF2525 at 39.705 kb on + strand, within GFF2525 at 39.738 kb on - strand, within GFF2525 at 39.903 kb on - strand, within GFF2525 at 39.903 kb on - strand, within GFF2525 at 39.999 kb on + strand, within GFF2525 at 40.188 kb on - strand, within GFF2525 at 40.357 kb on + strand at 40.378 kb on - strand at 40.392 kb on - strand at 40.397 kb on - strand at 40.397 kb on - strand at 40.542 kb on - strand at 40.543 kb on + strand at 40.722 kb on - strand, within GFF2526 at 41.322 kb on + strand, within GFF2527
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 38,440 + GFF2524 0.46 -0.2 38,860 - GFF2524 0.69 +0.6 38,860 - GFF2524 0.69 +1.1 39,074 + GFF2524 0.81 +0.5 39,079 + GFF2524 0.81 +0.5 39,091 + GFF2524 0.82 +0.4 39,296 - -0.7 39,296 - +0.9 39,608 + GFF2525 0.19 -3.2 39,705 + GFF2525 0.29 -1.1 39,738 - GFF2525 0.32 -0.1 39,903 - GFF2525 0.49 +0.5 39,903 - GFF2525 0.49 -0.5 39,999 + GFF2525 0.59 +0.2 40,188 - GFF2525 0.78 -2.0 40,357 + -0.5 40,378 - +2.1 40,392 - -0.1 40,397 - -3.1 40,397 - -2.1 40,542 - -3.3 40,543 + +1.2 40,722 - GFF2526 0.48 +0.0 41,322 + GFF2527 0.34 +1.3
Or see this region's nucleotide sequence