Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2289 and GFF2290 are separated by 97 nucleotides GFF2290 and GFF2291 are separated by 15 nucleotides GFF2291 and GFF2292 are separated by 89 nucleotides GFF2292 and GFF2293 are separated by 13 nucleotides
GFF2289 - Transcriptional regulator, GntR family, at 77,945 to 78,667
GFF2289
GFF2290 - Putative periplasmic protein, at 78,765 to 78,929
GFF2290
GFF2291 - Putative periplasmic protein, at 78,945 to 79,076
GFF2291
GFF2292 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 79,166 to 79,516
GFF2292
GFF2293 - Autoinducer 2 (AI-2) kinase LsrK (EC 2.7.1.-), at 79,530 to 81,080
GFF2293
Position (kb) 79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 78.191 kb on + strand, within GFF2289 at 78.192 kb on - strand, within GFF2289 at 78.211 kb on + strand, within GFF2289 at 78.330 kb on - strand, within GFF2289 at 78.330 kb on - strand, within GFF2289 at 78.337 kb on + strand, within GFF2289 at 78.337 kb on + strand, within GFF2289 at 78.373 kb on + strand, within GFF2289 at 78.388 kb on - strand, within GFF2289 at 78.389 kb on + strand, within GFF2289 at 78.445 kb on + strand, within GFF2289 at 78.445 kb on + strand, within GFF2289 at 78.446 kb on - strand, within GFF2289 at 78.446 kb on - strand, within GFF2289 at 78.465 kb on + strand, within GFF2289 at 78.554 kb on - strand, within GFF2289 at 78.700 kb on + strand at 78.701 kb on - strand at 78.712 kb on - strand at 78.764 kb on + strand at 78.765 kb on - strand at 78.765 kb on - strand at 78.819 kb on - strand, within GFF2290 at 79.167 kb on + strand at 79.264 kb on - strand, within GFF2292 at 79.289 kb on + strand, within GFF2292 at 79.313 kb on - strand, within GFF2292 at 79.353 kb on - strand, within GFF2292 at 79.358 kb on - strand, within GFF2292 at 79.378 kb on + strand, within GFF2292 at 79.379 kb on - strand, within GFF2292 at 79.381 kb on + strand, within GFF2292 at 79.382 kb on - strand, within GFF2292 at 79.382 kb on - strand, within GFF2292 at 79.418 kb on + strand, within GFF2292 at 79.418 kb on + strand, within GFF2292 at 79.418 kb on + strand, within GFF2292 at 79.652 kb on + strand at 79.653 kb on - strand at 79.776 kb on + strand, within GFF2293 at 79.776 kb on + strand, within GFF2293 at 79.944 kb on + strand, within GFF2293 at 79.959 kb on - strand, within GFF2293 at 79.988 kb on + strand, within GFF2293 at 79.989 kb on - strand, within GFF2293 at 80.009 kb on - strand, within GFF2293 at 80.009 kb on - strand, within GFF2293 at 80.101 kb on + strand, within GFF2293 at 80.111 kb on + strand, within GFF2293 at 80.112 kb on - strand, within GFF2293 at 80.115 kb on + strand, within GFF2293 at 80.131 kb on - strand, within GFF2293 at 80.195 kb on + strand, within GFF2293 at 80.208 kb on + strand, within GFF2293 at 80.355 kb on + strand, within GFF2293
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 78,191 + GFF2289 0.34 -0.9 78,192 - GFF2289 0.34 -0.8 78,211 + GFF2289 0.37 +2.2 78,330 - GFF2289 0.53 +1.3 78,330 - GFF2289 0.53 -1.0 78,337 + GFF2289 0.54 +0.9 78,337 + GFF2289 0.54 -1.5 78,373 + GFF2289 0.59 -0.5 78,388 - GFF2289 0.61 -0.9 78,389 + GFF2289 0.61 +1.1 78,445 + GFF2289 0.69 +0.2 78,445 + GFF2289 0.69 -0.4 78,446 - GFF2289 0.69 -0.2 78,446 - GFF2289 0.69 +0.5 78,465 + GFF2289 0.72 -2.1 78,554 - GFF2289 0.84 -1.0 78,700 + +0.4 78,701 - +1.3 78,712 - -0.1 78,764 + +0.7 78,765 - -0.3 78,765 - -2.4 78,819 - GFF2290 0.33 +1.6 79,167 + +0.3 79,264 - GFF2292 0.28 +1.4 79,289 + GFF2292 0.35 -0.2 79,313 - GFF2292 0.42 +0.2 79,353 - GFF2292 0.53 +0.3 79,358 - GFF2292 0.55 +2.8 79,378 + GFF2292 0.60 +0.7 79,379 - GFF2292 0.61 +0.2 79,381 + GFF2292 0.61 +1.2 79,382 - GFF2292 0.62 -2.7 79,382 - GFF2292 0.62 +1.1 79,418 + GFF2292 0.72 +0.5 79,418 + GFF2292 0.72 +0.8 79,418 + GFF2292 0.72 +0.1 79,652 + +0.3 79,653 - -0.4 79,776 + GFF2293 0.16 +0.4 79,776 + GFF2293 0.16 +1.9 79,944 + GFF2293 0.27 -0.3 79,959 - GFF2293 0.28 +1.3 79,988 + GFF2293 0.30 -0.8 79,989 - GFF2293 0.30 +0.0 80,009 - GFF2293 0.31 +0.2 80,009 - GFF2293 0.31 +0.5 80,101 + GFF2293 0.37 -1.1 80,111 + GFF2293 0.37 -3.1 80,112 - GFF2293 0.38 -1.1 80,115 + GFF2293 0.38 +0.7 80,131 - GFF2293 0.39 +0.1 80,195 + GFF2293 0.43 +0.6 80,208 + GFF2293 0.44 +0.4 80,355 + GFF2293 0.53 +0.1
Or see this region's nucleotide sequence