Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2268 and GFF2269 are separated by 3 nucleotides GFF2269 and GFF2270 are separated by 34 nucleotides GFF2270 and GFF2271 are separated by 194 nucleotides GFF2271 and GFF2272 are separated by 36 nucleotides
GFF2268 - L-rhamnose-proton symporter, at 59,823 to 60,857
GFF2268
GFF2269 - hypothetical protein, at 60,861 to 60,992
GFF2269
GFF2270 - hypothetical protein, at 61,027 to 61,140
GFF2270
GFF2271 - hypothetical protein, at 61,335 to 61,451
GFF2271
GFF2272 - Oligogalacturonide-Rhamnogalacturonide-specific porin, at 61,488 to 62,159
GFF2272
Position (kb)
60
61 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 59.868 kb on + strand at 59.886 kb on + strand at 59.889 kb on + strand at 59.890 kb on - strand at 59.890 kb on - strand at 59.929 kb on - strand, within GFF2268 at 59.962 kb on + strand, within GFF2268 at 59.962 kb on + strand, within GFF2268 at 59.963 kb on - strand, within GFF2268 at 59.963 kb on - strand, within GFF2268 at 59.993 kb on - strand, within GFF2268 at 59.993 kb on - strand, within GFF2268 at 59.998 kb on + strand, within GFF2268 at 59.998 kb on + strand, within GFF2268 at 60.031 kb on + strand, within GFF2268 at 60.226 kb on + strand, within GFF2268 at 60.227 kb on - strand, within GFF2268 at 60.227 kb on - strand, within GFF2268 at 60.475 kb on - strand, within GFF2268 at 60.475 kb on - strand, within GFF2268 at 60.488 kb on + strand, within GFF2268 at 60.501 kb on - strand, within GFF2268 at 60.501 kb on - strand, within GFF2268 at 60.531 kb on - strand, within GFF2268 at 60.589 kb on + strand, within GFF2268 at 60.594 kb on - strand, within GFF2268 at 60.594 kb on - strand, within GFF2268 at 60.594 kb on - strand, within GFF2268 at 60.667 kb on + strand, within GFF2268 at 60.790 kb on + strand at 60.938 kb on + strand, within GFF2269 at 61.078 kb on + strand, within GFF2270 at 61.602 kb on + strand, within GFF2272 at 61.719 kb on - strand, within GFF2272 at 61.736 kb on + strand, within GFF2272 at 61.801 kb on - strand, within GFF2272 at 61.801 kb on - strand, within GFF2272 at 61.873 kb on - strand, within GFF2272 at 61.920 kb on - strand, within GFF2272
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 59,868 + +1.0 59,886 + +0.0 59,889 + -0.8 59,890 - +0.5 59,890 - +0.5 59,929 - GFF2268 0.10 -1.0 59,962 + GFF2268 0.13 -0.4 59,962 + GFF2268 0.13 +1.7 59,963 - GFF2268 0.14 +0.6 59,963 - GFF2268 0.14 -0.1 59,993 - GFF2268 0.16 +0.2 59,993 - GFF2268 0.16 +0.1 59,998 + GFF2268 0.17 +0.8 59,998 + GFF2268 0.17 +0.6 60,031 + GFF2268 0.20 +0.9 60,226 + GFF2268 0.39 -0.3 60,227 - GFF2268 0.39 +0.9 60,227 - GFF2268 0.39 -2.1 60,475 - GFF2268 0.63 +0.5 60,475 - GFF2268 0.63 +0.4 60,488 + GFF2268 0.64 -0.3 60,501 - GFF2268 0.66 -3.2 60,501 - GFF2268 0.66 +0.9 60,531 - GFF2268 0.68 +1.3 60,589 + GFF2268 0.74 -0.4 60,594 - GFF2268 0.74 -1.0 60,594 - GFF2268 0.74 +1.6 60,594 - GFF2268 0.74 -4.1 60,667 + GFF2268 0.82 -1.9 60,790 + -1.9 60,938 + GFF2269 0.58 -1.7 61,078 + GFF2270 0.45 +0.6 61,602 + GFF2272 0.17 -0.4 61,719 - GFF2272 0.34 -1.4 61,736 + GFF2272 0.37 -0.0 61,801 - GFF2272 0.47 +0.3 61,801 - GFF2272 0.47 -0.3 61,873 - GFF2272 0.57 +0.0 61,920 - GFF2272 0.64 +1.8
Or see this region's nucleotide sequence