Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2193 and GFF2194 are separated by 127 nucleotides GFF2194 and GFF2195 are separated by 62 nucleotides GFF2195 and GFF2196 are separated by 19 nucleotides
GFF2193 - FIG01200701: possible membrane protein, at 44,359 to 45,402
GFF2193
GFF2194 - FIG01046050: hypothetical protein, at 45,530 to 45,760
GFF2194
GFF2195 - Transketolase (EC 2.2.1.1), at 45,823 to 47,823
GFF2195
GFF2196 - Transaldolase (EC 2.2.1.2), at 47,843 to 48,793
GFF2196
Position (kb) 45
46
47
48 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 45.058 kb on - strand, within GFF2193 at 45.094 kb on + strand, within GFF2193 at 45.097 kb on - strand, within GFF2193 at 45.156 kb on - strand, within GFF2193 at 45.174 kb on + strand, within GFF2193 at 45.354 kb on + strand at 45.382 kb on - strand at 45.464 kb on + strand at 45.474 kb on + strand at 45.613 kb on - strand, within GFF2194 at 45.838 kb on + strand at 45.962 kb on + strand at 45.962 kb on + strand at 45.983 kb on - strand at 46.138 kb on - strand, within GFF2195 at 46.174 kb on - strand, within GFF2195 at 46.508 kb on + strand, within GFF2195 at 46.538 kb on + strand, within GFF2195 at 46.539 kb on - strand, within GFF2195 at 46.614 kb on + strand, within GFF2195 at 46.617 kb on - strand, within GFF2195 at 46.617 kb on - strand, within GFF2195 at 46.662 kb on + strand, within GFF2195 at 47.189 kb on - strand, within GFF2195 at 47.314 kb on - strand, within GFF2195 at 47.378 kb on + strand, within GFF2195 at 47.378 kb on + strand, within GFF2195 at 47.378 kb on + strand, within GFF2195 at 47.384 kb on + strand, within GFF2195 at 47.384 kb on + strand, within GFF2195 at 47.433 kb on + strand, within GFF2195 at 47.610 kb on + strand, within GFF2195 at 47.613 kb on - strand, within GFF2195 at 47.663 kb on + strand at 47.686 kb on - strand at 47.686 kb on - strand at 47.708 kb on - strand at 47.823 kb on + strand at 47.823 kb on + strand at 47.846 kb on + strand at 48.067 kb on + strand, within GFF2196 at 48.214 kb on - strand, within GFF2196 at 48.230 kb on - strand, within GFF2196 at 48.344 kb on - strand, within GFF2196 at 48.559 kb on + strand, within GFF2196 at 48.683 kb on + strand, within GFF2196 at 48.683 kb on + strand, within GFF2196
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 45,058 - GFF2193 0.67 -0.2 45,094 + GFF2193 0.70 -0.1 45,097 - GFF2193 0.71 -2.6 45,156 - GFF2193 0.76 -1.3 45,174 + GFF2193 0.78 +0.5 45,354 + +0.3 45,382 - -0.8 45,464 + +0.1 45,474 + +2.3 45,613 - GFF2194 0.36 -0.4 45,838 + -0.5 45,962 + -0.3 45,962 + -0.6 45,983 - +0.3 46,138 - GFF2195 0.16 -0.8 46,174 - GFF2195 0.18 +0.4 46,508 + GFF2195 0.34 +0.0 46,538 + GFF2195 0.36 -3.2 46,539 - GFF2195 0.36 +0.0 46,614 + GFF2195 0.40 -4.7 46,617 - GFF2195 0.40 +1.2 46,617 - GFF2195 0.40 +0.1 46,662 + GFF2195 0.42 -0.3 47,189 - GFF2195 0.68 +0.5 47,314 - GFF2195 0.75 -0.7 47,378 + GFF2195 0.78 +0.6 47,378 + GFF2195 0.78 -0.9 47,378 + GFF2195 0.78 +1.9 47,384 + GFF2195 0.78 +1.1 47,384 + GFF2195 0.78 +0.9 47,433 + GFF2195 0.80 -1.3 47,610 + GFF2195 0.89 -0.1 47,613 - GFF2195 0.89 -0.3 47,663 + -0.9 47,686 - -0.1 47,686 - +0.2 47,708 - +2.9 47,823 + +0.6 47,823 + -3.7 47,846 + -0.3 48,067 + GFF2196 0.24 -0.6 48,214 - GFF2196 0.39 +0.1 48,230 - GFF2196 0.41 +0.5 48,344 - GFF2196 0.53 +0.0 48,559 + GFF2196 0.75 +0.6 48,683 + GFF2196 0.88 -1.5 48,683 + GFF2196 0.88 -2.1
Or see this region's nucleotide sequence