Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2172 and GFF2173 are separated by 70 nucleotides GFF2173 and GFF2174 are separated by 26 nucleotides GFF2174 and GFF2175 are separated by 39 nucleotides
GFF2172 - Uracil permease, at 19,439 to 20,728
GFF2172
GFF2173 - Chromosomal replication initiator protein DnaA, at 20,799 to 21,524
GFF2173
GFF2174 - Arsenate reductase (EC 1.20.4.1), at 21,551 to 21,910
GFF2174
GFF2175 - Exported zinc metalloprotease YfgC precursor, at 21,950 to 23,413
GFF2175
Position (kb) 20
21
22 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 20.016 kb on - strand, within GFF2172 at 20.300 kb on + strand, within GFF2172 at 20.301 kb on - strand, within GFF2172 at 20.301 kb on - strand, within GFF2172 at 20.462 kb on + strand, within GFF2172 at 20.691 kb on + strand at 20.774 kb on + strand at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.956 kb on - strand, within GFF2173 at 20.957 kb on + strand, within GFF2173 at 20.957 kb on + strand, within GFF2173 at 20.961 kb on + strand, within GFF2173 at 21.183 kb on + strand, within GFF2173 at 21.251 kb on - strand, within GFF2173 at 21.760 kb on + strand, within GFF2174 at 21.761 kb on - strand, within GFF2174 at 21.889 kb on - strand at 21.964 kb on - strand at 21.984 kb on - strand at 22.104 kb on + strand, within GFF2175 at 22.209 kb on + strand, within GFF2175 at 22.275 kb on - strand, within GFF2175
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 20,016 - GFF2172 0.45 +1.4 20,300 + GFF2172 0.67 -0.5 20,301 - GFF2172 0.67 -0.4 20,301 - GFF2172 0.67 +0.2 20,462 + GFF2172 0.79 -0.8 20,691 + -0.4 20,774 + +0.1 20,955 + GFF2173 0.21 -3.2 20,955 + GFF2173 0.21 -4.6 20,955 + GFF2173 0.21 -2.2 20,955 + GFF2173 0.21 -1.7 20,956 - GFF2173 0.22 -0.7 20,957 + GFF2173 0.22 -3.6 20,957 + GFF2173 0.22 -2.5 20,961 + GFF2173 0.22 -2.6 21,183 + GFF2173 0.53 -3.5 21,251 - GFF2173 0.62 -2.5 21,760 + GFF2174 0.58 -0.3 21,761 - GFF2174 0.58 +0.4 21,889 - -3.9 21,964 - -1.5 21,984 - -0.5 22,104 + GFF2175 0.11 +0.2 22,209 + GFF2175 0.18 -1.2 22,275 - GFF2175 0.22 +0.1
Or see this region's nucleotide sequence