Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1992 and GFF1993 are separated by 66 nucleotides GFF1993 and GFF1994 are separated by 51 nucleotides
GFF1992 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 3,915 to 7,241
GFF1992
GFF1993 - FIG00639836: hypothetical protein, at 7,308 to 7,622
GFF1993
GFF1994 - Putative amino acid permease, at 7,674 to 9,176
GFF1994
Position (kb)
7
8 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6.402 kb on - strand, within GFF1992 at 6.402 kb on - strand, within GFF1992 at 6.437 kb on + strand, within GFF1992 at 6.461 kb on + strand, within GFF1992 at 6.493 kb on + strand, within GFF1992 at 6.494 kb on - strand, within GFF1992 at 6.613 kb on + strand, within GFF1992 at 6.614 kb on - strand, within GFF1992 at 6.807 kb on - strand, within GFF1992 at 6.816 kb on - strand, within GFF1992 at 6.892 kb on + strand, within GFF1992 at 7.043 kb on + strand at 7.044 kb on - strand at 7.044 kb on - strand at 7.070 kb on + strand at 7.070 kb on + strand at 7.071 kb on - strand at 7.134 kb on - strand at 7.134 kb on - strand at 7.134 kb on - strand at 7.134 kb on - strand at 7.178 kb on + strand at 7.281 kb on + strand at 7.282 kb on - strand at 7.299 kb on + strand at 7.304 kb on + strand at 7.324 kb on + strand at 7.359 kb on + strand, within GFF1993 at 7.396 kb on - strand, within GFF1993 at 7.396 kb on - strand, within GFF1993 at 7.405 kb on + strand, within GFF1993 at 7.625 kb on + strand at 7.640 kb on + strand at 7.654 kb on + strand at 7.717 kb on + strand at 7.726 kb on + strand at 7.823 kb on + strand at 8.219 kb on + strand, within GFF1994 at 8.326 kb on + strand, within GFF1994 at 8.326 kb on + strand, within GFF1994 at 8.340 kb on + strand, within GFF1994 at 8.346 kb on - strand, within GFF1994 at 8.357 kb on + strand, within GFF1994 at 8.358 kb on - strand, within GFF1994 at 8.361 kb on + strand, within GFF1994 at 8.362 kb on - strand, within GFF1994
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 6,402 - GFF1992 0.75 +1.7 6,402 - GFF1992 0.75 -0.0 6,437 + GFF1992 0.76 +0.1 6,461 + GFF1992 0.77 +0.0 6,493 + GFF1992 0.77 +0.4 6,494 - GFF1992 0.78 -0.5 6,613 + GFF1992 0.81 -0.6 6,614 - GFF1992 0.81 +0.7 6,807 - GFF1992 0.87 +0.1 6,816 - GFF1992 0.87 +0.5 6,892 + GFF1992 0.89 +0.1 7,043 + +0.8 7,044 - +0.6 7,044 - +0.2 7,070 + -0.0 7,070 + +1.0 7,071 - -2.0 7,134 - -0.1 7,134 - +0.7 7,134 - -3.5 7,134 - -0.5 7,178 + +0.3 7,281 + -0.5 7,282 - -0.2 7,299 + -2.0 7,304 + +0.0 7,324 + -1.4 7,359 + GFF1993 0.16 +0.2 7,396 - GFF1993 0.28 +0.4 7,396 - GFF1993 0.28 +0.8 7,405 + GFF1993 0.31 +0.4 7,625 + +2.1 7,640 + +0.1 7,654 + -3.2 7,717 + -1.5 7,726 + +0.4 7,823 + -0.2 8,219 + GFF1994 0.36 -4.3 8,326 + GFF1994 0.43 -3.0 8,326 + GFF1994 0.43 +0.6 8,340 + GFF1994 0.44 -1.9 8,346 - GFF1994 0.45 +0.2 8,357 + GFF1994 0.45 +0.4 8,358 - GFF1994 0.46 -0.8 8,361 + GFF1994 0.46 -0.1 8,362 - GFF1994 0.46 -0.5
Or see this region's nucleotide sequence