Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1954 and GFF1955 overlap by 1 nucleotides GFF1955 and GFF1956 are separated by 177 nucleotides GFF1956 and GFF1957 are separated by 370 nucleotides
GFF1954 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 144,495 to 146,642
GFF1954
GFF1955 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 146,642 to 147,952
GFF1955
GFF1956 - FIG00637865: hypothetical protein, at 148,130 to 148,414
GFF1956
GFF1957 - Long-chain fatty acid transport protein, at 148,785 to 150,092
GFF1957
Position (kb)
146
147
148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 145.917 kb on - strand, within GFF1954 at 145.959 kb on + strand, within GFF1954 at 145.991 kb on + strand, within GFF1954 at 146.246 kb on + strand, within GFF1954 at 146.247 kb on - strand, within GFF1954 at 146.421 kb on + strand, within GFF1954 at 146.433 kb on - strand at 146.454 kb on - strand at 146.785 kb on - strand, within GFF1955 at 146.891 kb on + strand, within GFF1955 at 147.006 kb on - strand, within GFF1955 at 147.006 kb on - strand, within GFF1955 at 147.055 kb on - strand, within GFF1955 at 147.265 kb on - strand, within GFF1955 at 147.265 kb on - strand, within GFF1955 at 147.365 kb on - strand, within GFF1955 at 147.365 kb on - strand, within GFF1955 at 147.369 kb on + strand, within GFF1955 at 147.370 kb on - strand, within GFF1955 at 147.669 kb on - strand, within GFF1955 at 147.728 kb on - strand, within GFF1955 at 147.750 kb on - strand, within GFF1955 at 147.752 kb on + strand, within GFF1955 at 147.904 kb on + strand at 147.905 kb on - strand at 147.905 kb on - strand at 147.956 kb on - strand at 148.053 kb on - strand at 148.417 kb on + strand at 148.639 kb on + strand at 148.765 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 145,917 - GFF1954 0.66 -2.9 145,959 + GFF1954 0.68 +1.0 145,991 + GFF1954 0.70 +1.4 146,246 + GFF1954 0.82 -0.7 146,247 - GFF1954 0.82 +1.0 146,421 + GFF1954 0.90 -0.1 146,433 - -1.2 146,454 - +0.1 146,785 - GFF1955 0.11 +0.3 146,891 + GFF1955 0.19 +0.4 147,006 - GFF1955 0.28 +1.7 147,006 - GFF1955 0.28 +1.2 147,055 - GFF1955 0.32 +0.0 147,265 - GFF1955 0.48 +1.0 147,265 - GFF1955 0.48 -2.1 147,365 - GFF1955 0.55 -1.4 147,365 - GFF1955 0.55 +1.8 147,369 + GFF1955 0.55 +0.8 147,370 - GFF1955 0.56 +0.6 147,669 - GFF1955 0.78 +0.8 147,728 - GFF1955 0.83 +0.4 147,750 - GFF1955 0.85 +2.0 147,752 + GFF1955 0.85 +0.2 147,904 + +0.3 147,905 - +0.6 147,905 - -1.4 147,956 - -0.8 148,053 - +0.2 148,417 + -0.3 148,639 + +1.3 148,765 + -0.9
Or see this region's nucleotide sequence