Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1868 and GFF1869 are separated by 14 nucleotides GFF1869 and GFF1870 overlap by 4 nucleotides GFF1870 and GFF1871 are separated by 85 nucleotides
GFF1868 - Naphthoate synthase (EC 4.1.3.36), at 60,392 to 61,249
GFF1868
GFF1869 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1- carboxylate synthase (EC 4.2.99.20), at 61,264 to 62,022
GFF1869
GFF1870 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase (EC 2.2.1.9), at 62,019 to 63,689
GFF1870
GFF1871 - Menaquinone-specific isochorismate synthase (EC 5.4.4.2), at 63,775 to 65,070
GFF1871
Position (kb) 62
63
64 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 61.034 kb on - strand, within GFF1868 at 61.409 kb on - strand, within GFF1869 at 61.799 kb on + strand, within GFF1869 at 61.812 kb on + strand, within GFF1869 at 61.892 kb on + strand, within GFF1869 at 62.022 kb on + strand at 62.022 kb on + strand at 62.022 kb on + strand at 62.022 kb on + strand at 62.022 kb on + strand at 62.178 kb on - strand at 62.224 kb on + strand, within GFF1870 at 62.224 kb on + strand, within GFF1870 at 62.224 kb on + strand, within GFF1870 at 62.325 kb on + strand, within GFF1870 at 62.462 kb on - strand, within GFF1870 at 62.650 kb on - strand, within GFF1870 at 62.650 kb on - strand, within GFF1870 at 62.828 kb on + strand, within GFF1870 at 62.935 kb on - strand, within GFF1870 at 63.167 kb on - strand, within GFF1870 at 63.548 kb on + strand at 63.745 kb on + strand at 63.799 kb on - strand at 63.824 kb on - strand at 63.916 kb on + strand, within GFF1871 at 63.917 kb on - strand, within GFF1871 at 63.953 kb on + strand, within GFF1871 at 63.954 kb on - strand, within GFF1871 at 63.971 kb on + strand, within GFF1871 at 63.971 kb on + strand, within GFF1871 at 64.107 kb on + strand, within GFF1871 at 64.219 kb on - strand, within GFF1871 at 64.275 kb on + strand, within GFF1871 at 64.276 kb on - strand, within GFF1871 at 64.478 kb on - strand, within GFF1871 at 64.623 kb on + strand, within GFF1871 at 64.624 kb on - strand, within GFF1871
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 61,034 - GFF1868 0.75 -0.3 61,409 - GFF1869 0.19 -1.3 61,799 + GFF1869 0.70 +1.5 61,812 + GFF1869 0.72 +1.1 61,892 + GFF1869 0.83 +0.8 62,022 + -1.4 62,022 + +0.4 62,022 + +0.2 62,022 + +1.5 62,022 + +0.3 62,178 - +0.4 62,224 + GFF1870 0.12 -0.1 62,224 + GFF1870 0.12 -0.4 62,224 + GFF1870 0.12 -2.2 62,325 + GFF1870 0.18 +2.1 62,462 - GFF1870 0.27 +1.2 62,650 - GFF1870 0.38 +3.7 62,650 - GFF1870 0.38 +2.1 62,828 + GFF1870 0.48 +3.0 62,935 - GFF1870 0.55 +1.2 63,167 - GFF1870 0.69 +0.2 63,548 + -3.1 63,745 + +0.4 63,799 - -3.1 63,824 - +0.6 63,916 + GFF1871 0.11 +1.6 63,917 - GFF1871 0.11 +1.0 63,953 + GFF1871 0.14 -1.0 63,954 - GFF1871 0.14 -1.6 63,971 + GFF1871 0.15 -2.4 63,971 + GFF1871 0.15 +0.4 64,107 + GFF1871 0.26 +2.7 64,219 - GFF1871 0.34 +0.0 64,275 + GFF1871 0.39 -0.1 64,276 - GFF1871 0.39 -0.6 64,478 - GFF1871 0.54 +1.2 64,623 + GFF1871 0.65 +0.1 64,624 - GFF1871 0.66 +0.3
Or see this region's nucleotide sequence