Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1714 and GFF1715 are separated by 89 nucleotides GFF1715 and GFF1716 are separated by 378 nucleotides GFF1716 and GFF1717 are separated by 102 nucleotides
GFF1714 - Regulatory protein AsnC, at 14,682 to 15,140
GFF1714
GFF1715 - Flavoprotein MioC, at 15,230 to 15,673
GFF1715
GFF1716 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 16,052 to 17,941
GFF1716
GFF1717 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 18,044 to 18,667
GFF1717
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 15.197 kb on - strand at 15.241 kb on + strand at 15.241 kb on + strand at 15.242 kb on - strand at 15.242 kb on - strand at 15.351 kb on + strand, within GFF1715 at 15.477 kb on + strand, within GFF1715 at 15.513 kb on - strand, within GFF1715 at 15.513 kb on - strand, within GFF1715 at 15.993 kb on + strand at 16.048 kb on + strand at 16.132 kb on + strand at 16.165 kb on + strand at 16.359 kb on + strand, within GFF1716 at 16.360 kb on - strand, within GFF1716 at 16.559 kb on + strand, within GFF1716 at 16.559 kb on + strand, within GFF1716 at 16.664 kb on - strand, within GFF1716 at 16.789 kb on + strand, within GFF1716 at 16.790 kb on - strand, within GFF1716 at 16.820 kb on - strand, within GFF1716 at 16.845 kb on - strand, within GFF1716 at 16.845 kb on - strand, within GFF1716 at 16.921 kb on + strand, within GFF1716 at 16.974 kb on - strand, within GFF1716 at 16.984 kb on + strand, within GFF1716 at 16.984 kb on + strand, within GFF1716 at 17.042 kb on - strand, within GFF1716 at 17.338 kb on + strand, within GFF1716 at 17.610 kb on - strand, within GFF1716 at 17.702 kb on + strand, within GFF1716 at 17.739 kb on + strand, within GFF1716 at 17.739 kb on + strand, within GFF1716 at 17.753 kb on - strand at 17.753 kb on - strand at 17.753 kb on - strand at 17.830 kb on + strand at 17.830 kb on + strand at 17.831 kb on - strand at 17.920 kb on + strand at 17.920 kb on + strand at 17.921 kb on - strand at 17.921 kb on - strand at 17.921 kb on - strand at 17.948 kb on + strand at 17.949 kb on - strand at 18.122 kb on + strand, within GFF1717 at 18.130 kb on + strand, within GFF1717 at 18.130 kb on + strand, within GFF1717 at 18.155 kb on + strand, within GFF1717 at 18.155 kb on + strand, within GFF1717 at 18.155 kb on + strand, within GFF1717 at 18.156 kb on - strand, within GFF1717 at 18.216 kb on + strand, within GFF1717 at 18.482 kb on - strand, within GFF1717 at 18.584 kb on - strand, within GFF1717
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 15,197 - -0.1 15,241 + -2.4 15,241 + +2.2 15,242 - -0.8 15,242 - +1.6 15,351 + GFF1715 0.27 -1.1 15,477 + GFF1715 0.56 +0.3 15,513 - GFF1715 0.64 +0.3 15,513 - GFF1715 0.64 -0.7 15,993 + -0.1 16,048 + -0.7 16,132 + -1.2 16,165 + -1.7 16,359 + GFF1716 0.16 -4.4 16,360 - GFF1716 0.16 -3.3 16,559 + GFF1716 0.27 -2.6 16,559 + GFF1716 0.27 -2.7 16,664 - GFF1716 0.32 -3.6 16,789 + GFF1716 0.39 -2.7 16,790 - GFF1716 0.39 -1.7 16,820 - GFF1716 0.41 -2.8 16,845 - GFF1716 0.42 -3.4 16,845 - GFF1716 0.42 -1.4 16,921 + GFF1716 0.46 -2.7 16,974 - GFF1716 0.49 -2.7 16,984 + GFF1716 0.49 -2.3 16,984 + GFF1716 0.49 -2.6 17,042 - GFF1716 0.52 -3.4 17,338 + GFF1716 0.68 -2.6 17,610 - GFF1716 0.82 -4.1 17,702 + GFF1716 0.87 -3.2 17,739 + GFF1716 0.89 -2.5 17,739 + GFF1716 0.89 -2.6 17,753 - -2.3 17,753 - -3.3 17,753 - -3.9 17,830 + -1.7 17,830 + -3.0 17,831 - -2.9 17,920 + +0.2 17,920 + +0.4 17,921 - +3.0 17,921 - +0.1 17,921 - +0.5 17,948 + +0.9 17,949 - -0.0 18,122 + GFF1717 0.12 +0.8 18,130 + GFF1717 0.14 +0.5 18,130 + GFF1717 0.14 -1.5 18,155 + GFF1717 0.18 -2.4 18,155 + GFF1717 0.18 +2.3 18,155 + GFF1717 0.18 -1.0 18,156 - GFF1717 0.18 -0.7 18,216 + GFF1717 0.28 +0.9 18,482 - GFF1717 0.70 +1.2 18,584 - GFF1717 0.87 -0.7
Or see this region's nucleotide sequence