Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1690 and GFF1691 are separated by 88 nucleotides GFF1691 and GFF1692 are separated by 146 nucleotides GFF1692 and GFF1693 are separated by 153 nucleotides
GFF1690 - RNA polymerase sigma factor for flagellar operon, at 215 to 934
GFF1690
GFF1691 - Flagellar biosynthesis protein FliZ, at 1,023 to 1,544
GFF1691
GFF1692 - Cystine ABC transporter, periplasmic cystine-binding protein FliY, at 1,691 to 2,491
GFF1692
GFF1693 - D-cysteine desulfhydrase (EC 4.4.1.15), at 2,645 to 3,631
GFF1693
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 0.812 kb on + strand, within GFF1690 at 0.813 kb on - strand, within GFF1690 at 0.925 kb on + strand at 0.979 kb on + strand at 0.980 kb on - strand at 0.980 kb on - strand at 1.127 kb on + strand, within GFF1691 at 1.157 kb on - strand, within GFF1691 at 1.320 kb on + strand, within GFF1691 at 1.351 kb on - strand, within GFF1691 at 1.463 kb on + strand, within GFF1691 at 1.464 kb on - strand, within GFF1691 at 1.535 kb on + strand at 1.536 kb on - strand at 1.595 kb on + strand at 1.601 kb on - strand at 1.677 kb on + strand at 1.688 kb on - strand at 1.691 kb on - strand at 2.060 kb on + strand, within GFF1692 at 2.060 kb on + strand, within GFF1692 at 2.062 kb on + strand, within GFF1692 at 2.168 kb on + strand, within GFF1692 at 2.169 kb on - strand, within GFF1692 at 2.378 kb on - strand, within GFF1692 at 2.378 kb on - strand, within GFF1692 at 2.407 kb on - strand, within GFF1692 at 2.410 kb on + strand, within GFF1692 at 2.542 kb on + strand at 2.543 kb on - strand at 2.545 kb on + strand at 2.545 kb on + strand at 2.546 kb on - strand at 2.546 kb on - strand at 2.608 kb on - strand at 2.652 kb on + strand at 2.750 kb on + strand, within GFF1693 at 3.104 kb on + strand, within GFF1693 at 3.153 kb on + strand, within GFF1693 at 3.249 kb on + strand, within GFF1693 at 3.355 kb on + strand, within GFF1693 at 3.400 kb on - strand, within GFF1693 at 3.411 kb on - strand, within GFF1693
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 812 + GFF1690 0.83 -1.8 813 - GFF1690 0.83 -0.1 925 + +0.6 979 + -0.1 980 - -0.7 980 - -0.4 1,127 + GFF1691 0.20 -0.7 1,157 - GFF1691 0.26 -1.8 1,320 + GFF1691 0.57 -0.3 1,351 - GFF1691 0.63 +0.5 1,463 + GFF1691 0.84 -0.7 1,464 - GFF1691 0.84 -1.9 1,535 + +0.3 1,536 - +0.2 1,595 + +0.2 1,601 - -0.7 1,677 + +0.6 1,688 - -0.7 1,691 - +0.5 2,060 + GFF1692 0.46 +0.5 2,060 + GFF1692 0.46 -1.3 2,062 + GFF1692 0.46 -2.5 2,168 + GFF1692 0.60 -1.4 2,169 - GFF1692 0.60 +0.0 2,378 - GFF1692 0.86 -1.5 2,378 - GFF1692 0.86 -0.5 2,407 - GFF1692 0.89 -1.5 2,410 + GFF1692 0.90 -0.2 2,542 + -1.5 2,543 - -4.0 2,545 + -1.2 2,545 + -0.5 2,546 - -1.7 2,546 - -0.5 2,608 - -0.4 2,652 + -0.8 2,750 + GFF1693 0.11 -0.6 3,104 + GFF1693 0.47 +1.1 3,153 + GFF1693 0.51 +0.4 3,249 + GFF1693 0.61 -0.1 3,355 + GFF1693 0.72 -0.9 3,400 - GFF1693 0.76 -0.6 3,411 - GFF1693 0.78 +0.4
Or see this region's nucleotide sequence