Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1678

Experiment: Ffm_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1677 and GFF1678 are separated by 550 nucleotidesGFF1678 and GFF1679 are separated by 178 nucleotides GFF1677 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 62,553 to 63,110 GFF1677 GFF1678 - Ferritin-like protein 2, at 63,661 to 64,164 GFF1678 GFF1679 - predicted 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17), at 64,343 to 64,981 GFF1679 Position (kb) 63 64 65Strain fitness (log2 ratio) -2 -1 0 1at 62.704 kb on + strand, within GFF1677at 62.750 kb on - strand, within GFF1677at 62.904 kb on + strand, within GFF1677at 62.969 kb on + strand, within GFF1677at 62.970 kb on - strand, within GFF1677at 63.053 kb on + strand, within GFF1677at 63.054 kb on - strand, within GFF1677at 63.172 kb on - strandat 63.561 kb on + strandat 63.731 kb on - strand, within GFF1678at 63.741 kb on - strand, within GFF1678at 64.092 kb on + strand, within GFF1678at 64.151 kb on - strandat 64.151 kb on - strandat 64.197 kb on + strandat 64.198 kb on - strandat 64.830 kb on - strand, within GFF1679at 64.927 kb on - strandat 64.927 kb on - strandat 65.018 kb on - strandat 65.126 kb on + strandat 65.142 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay
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62,704 + GFF1677 0.27 +0.1
62,750 - GFF1677 0.35 +0.6
62,904 + GFF1677 0.63 +1.7
62,969 + GFF1677 0.75 +0.0
62,970 - GFF1677 0.75 -0.7
63,053 + GFF1677 0.90 +0.0
63,054 - GFF1677 0.90 +0.3
63,172 - +0.3
63,561 + -0.2
63,731 - GFF1678 0.14 -1.2
63,741 - GFF1678 0.16 -1.3
64,092 + GFF1678 0.86 +0.5
64,151 - +0.8
64,151 - +1.5
64,197 + -0.0
64,198 - -1.0
64,830 - GFF1679 0.76 -0.5
64,927 - -2.1
64,927 - +0.0
65,018 - +0.5
65,126 + +1.7
65,142 - +1.1

Or see this region's nucleotide sequence