Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1397 and GFF1398 are separated by 63 nucleotides GFF1398 and GFF1399 overlap by 4 nucleotides GFF1399 and GFF1400 overlap by 1 nucleotides
GFF1397 - YjbE secreted protein, at 42,382 to 42,627
GFF1397
GFF1398 - YjbF outer membrane lipoprotein, at 42,691 to 43,329
GFF1398
GFF1399 - YjbG polysaccharide synthesis-related protein, at 43,326 to 44,063
GFF1399
GFF1400 - YjbH outer membrane lipoprotein, at 44,063 to 46,159
GFF1400
Position (kb) 43
44
45 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 42.400 kb on + strand at 42.533 kb on + strand, within GFF1397 at 42.533 kb on + strand, within GFF1397 at 42.534 kb on - strand, within GFF1397 at 42.641 kb on - strand at 42.725 kb on + strand at 42.835 kb on + strand, within GFF1398 at 43.005 kb on + strand, within GFF1398 at 43.005 kb on + strand, within GFF1398 at 43.125 kb on + strand, within GFF1398 at 43.185 kb on - strand, within GFF1398 at 43.185 kb on - strand, within GFF1398 at 43.271 kb on - strand at 43.731 kb on + strand, within GFF1399 at 43.732 kb on - strand, within GFF1399 at 43.833 kb on + strand, within GFF1399 at 43.876 kb on - strand, within GFF1399 at 43.934 kb on + strand, within GFF1399 at 43.934 kb on + strand, within GFF1399 at 43.943 kb on + strand, within GFF1399 at 43.944 kb on - strand, within GFF1399 at 44.061 kb on + strand at 44.061 kb on + strand at 44.308 kb on + strand, within GFF1400 at 44.320 kb on + strand, within GFF1400 at 44.366 kb on + strand, within GFF1400 at 44.480 kb on + strand, within GFF1400 at 44.481 kb on - strand, within GFF1400 at 44.483 kb on - strand, within GFF1400 at 44.742 kb on - strand, within GFF1400 at 44.742 kb on - strand, within GFF1400 at 44.852 kb on - strand, within GFF1400 at 44.858 kb on - strand, within GFF1400 at 44.895 kb on + strand, within GFF1400 at 45.048 kb on + strand, within GFF1400 at 45.050 kb on + strand, within GFF1400 at 45.051 kb on - strand, within GFF1400 at 45.051 kb on - strand, within GFF1400 at 45.051 kb on - strand, within GFF1400
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 42,400 + -0.5 42,533 + GFF1397 0.61 -0.6 42,533 + GFF1397 0.61 -0.4 42,534 - GFF1397 0.62 -4.4 42,641 - +1.0 42,725 + -2.9 42,835 + GFF1398 0.23 -0.2 43,005 + GFF1398 0.49 +0.5 43,005 + GFF1398 0.49 +0.5 43,125 + GFF1398 0.68 -0.5 43,185 - GFF1398 0.77 +0.7 43,185 - GFF1398 0.77 -0.1 43,271 - +0.1 43,731 + GFF1399 0.55 +1.1 43,732 - GFF1399 0.55 +0.9 43,833 + GFF1399 0.69 +0.6 43,876 - GFF1399 0.75 -2.8 43,934 + GFF1399 0.82 +0.3 43,934 + GFF1399 0.82 +0.9 43,943 + GFF1399 0.84 -0.4 43,944 - GFF1399 0.84 -2.8 44,061 + +0.2 44,061 + +1.1 44,308 + GFF1400 0.12 +1.2 44,320 + GFF1400 0.12 +0.7 44,366 + GFF1400 0.14 -0.1 44,480 + GFF1400 0.20 -3.6 44,481 - GFF1400 0.20 -5.1 44,483 - GFF1400 0.20 -0.5 44,742 - GFF1400 0.32 -0.5 44,742 - GFF1400 0.32 -0.9 44,852 - GFF1400 0.38 -0.4 44,858 - GFF1400 0.38 -0.5 44,895 + GFF1400 0.40 -1.4 45,048 + GFF1400 0.47 +0.7 45,050 + GFF1400 0.47 -0.3 45,051 - GFF1400 0.47 -0.8 45,051 - GFF1400 0.47 -0.7 45,051 - GFF1400 0.47 +0.5
Or see this region's nucleotide sequence