Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1330 and GFF1331 are separated by 122 nucleotides GFF1331 and GFF1332 are separated by 103 nucleotides GFF1332 and GFF1333 are separated by 141 nucleotides
GFF1330 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 38,466 to 39,644
GFF1330
GFF1331 - LysR-family transcriptional regulator STM3020, at 39,767 to 40,630
GFF1331
GFF1332 - FIG01045951: hypothetical protein, at 40,734 to 41,189
GFF1332
GFF1333 - Serine transporter, at 41,331 to 42,560
GFF1333
Position (kb) 39
40
41 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 38.767 kb on - strand, within GFF1330 at 38.913 kb on - strand, within GFF1330 at 38.931 kb on + strand, within GFF1330 at 38.936 kb on + strand, within GFF1330 at 38.936 kb on + strand, within GFF1330 at 39.026 kb on + strand, within GFF1330 at 39.026 kb on + strand, within GFF1330 at 39.112 kb on + strand, within GFF1330 at 39.113 kb on - strand, within GFF1330 at 39.113 kb on - strand, within GFF1330 at 39.213 kb on + strand, within GFF1330 at 39.294 kb on - strand, within GFF1330 at 39.398 kb on + strand, within GFF1330 at 39.398 kb on + strand, within GFF1330 at 39.478 kb on - strand, within GFF1330 at 39.478 kb on - strand, within GFF1330 at 39.769 kb on - strand at 39.969 kb on - strand, within GFF1331 at 39.971 kb on + strand, within GFF1331 at 39.972 kb on - strand, within GFF1331 at 40.007 kb on - strand, within GFF1331 at 40.058 kb on - strand, within GFF1331 at 40.058 kb on - strand, within GFF1331 at 40.116 kb on + strand, within GFF1331 at 40.116 kb on + strand, within GFF1331 at 40.117 kb on - strand, within GFF1331 at 40.196 kb on + strand, within GFF1331 at 40.197 kb on - strand, within GFF1331 at 40.267 kb on + strand, within GFF1331 at 40.298 kb on + strand, within GFF1331 at 40.425 kb on + strand, within GFF1331 at 40.425 kb on + strand, within GFF1331 at 40.426 kb on - strand, within GFF1331 at 40.429 kb on + strand, within GFF1331 at 40.429 kb on + strand, within GFF1331 at 40.430 kb on - strand, within GFF1331 at 40.430 kb on - strand, within GFF1331 at 40.430 kb on - strand, within GFF1331 at 40.604 kb on + strand at 40.607 kb on + strand at 40.607 kb on + strand at 40.635 kb on + strand at 40.639 kb on + strand at 41.063 kb on - strand, within GFF1332 at 41.190 kb on + strand at 41.276 kb on - strand at 41.379 kb on + strand at 41.379 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 38,767 - GFF1330 0.26 +0.2 38,913 - GFF1330 0.38 +0.8 38,931 + GFF1330 0.39 -1.9 38,936 + GFF1330 0.40 +1.4 38,936 + GFF1330 0.40 +0.2 39,026 + GFF1330 0.47 +0.4 39,026 + GFF1330 0.47 +0.7 39,112 + GFF1330 0.55 +0.3 39,113 - GFF1330 0.55 +1.2 39,113 - GFF1330 0.55 +0.2 39,213 + GFF1330 0.63 +2.1 39,294 - GFF1330 0.70 +0.4 39,398 + GFF1330 0.79 +0.5 39,398 + GFF1330 0.79 +0.8 39,478 - GFF1330 0.86 +0.9 39,478 - GFF1330 0.86 +0.3 39,769 - -0.0 39,969 - GFF1331 0.23 +0.2 39,971 + GFF1331 0.24 -2.0 39,972 - GFF1331 0.24 +0.6 40,007 - GFF1331 0.28 -0.5 40,058 - GFF1331 0.34 -3.2 40,058 - GFF1331 0.34 -1.8 40,116 + GFF1331 0.40 +0.3 40,116 + GFF1331 0.40 +1.6 40,117 - GFF1331 0.41 -2.2 40,196 + GFF1331 0.50 +2.0 40,197 - GFF1331 0.50 -3.4 40,267 + GFF1331 0.58 +0.0 40,298 + GFF1331 0.61 -0.1 40,425 + GFF1331 0.76 +0.0 40,425 + GFF1331 0.76 +0.4 40,426 - GFF1331 0.76 -1.4 40,429 + GFF1331 0.77 -1.6 40,429 + GFF1331 0.77 +1.1 40,430 - GFF1331 0.77 -0.0 40,430 - GFF1331 0.77 +0.7 40,430 - GFF1331 0.77 +0.6 40,604 + -1.9 40,607 + +0.5 40,607 + +0.0 40,635 + +1.2 40,639 + +0.6 41,063 - GFF1332 0.72 +0.6 41,190 + +0.1 41,276 - -0.4 41,379 + +0.3 41,379 + +0.8
Or see this region's nucleotide sequence