Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1237 and GFF1238 overlap by 38 nucleotides GFF1238 and GFF1239 are separated by 173 nucleotides GFF1239 and GFF1240 are separated by 173 nucleotides
GFF1237 - FIG01046247: hypothetical protein, at 70,235 to 70,402
GFF1237
GFF1238 - 28.1 kDa virulence protein, at 70,365 to 71,132
GFF1238
GFF1239 - Gifsy-2 prophage lysozyme( EC:3.2.1.17 ), at 71,306 to 71,470
GFF1239
GFF1240 - Virulence genes transcriptional activator, at 71,644 to 72,537
GFF1240
Position (kb) 71
72 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 70.346 kb on - strand, within GFF1237 at 70.346 kb on - strand, within GFF1237 at 70.367 kb on - strand at 70.424 kb on + strand at 70.432 kb on - strand at 70.469 kb on + strand, within GFF1238 at 70.505 kb on - strand, within GFF1238 at 70.597 kb on + strand, within GFF1238 at 70.597 kb on + strand, within GFF1238 at 70.598 kb on - strand, within GFF1238 at 70.598 kb on - strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.602 kb on - strand, within GFF1238 at 70.602 kb on - strand, within GFF1238 at 70.602 kb on - strand, within GFF1238 at 70.602 kb on - strand, within GFF1238 at 70.664 kb on + strand, within GFF1238 at 70.665 kb on - strand, within GFF1238 at 70.665 kb on - strand, within GFF1238 at 70.701 kb on + strand, within GFF1238 at 70.729 kb on + strand, within GFF1238 at 70.927 kb on + strand, within GFF1238 at 70.928 kb on - strand, within GFF1238 at 70.989 kb on - strand, within GFF1238 at 70.989 kb on - strand, within GFF1238 at 71.132 kb on + strand at 71.318 kb on + strand at 71.412 kb on + strand, within GFF1239 at 71.461 kb on + strand at 71.476 kb on - strand at 71.479 kb on + strand at 71.536 kb on - strand at 71.557 kb on + strand at 71.558 kb on - strand at 71.558 kb on - strand at 71.683 kb on + strand at 71.686 kb on - strand at 71.729 kb on + strand at 71.740 kb on - strand, within GFF1240 at 71.819 kb on - strand, within GFF1240 at 71.872 kb on - strand, within GFF1240 at 71.895 kb on + strand, within GFF1240 at 71.899 kb on - strand, within GFF1240 at 71.902 kb on + strand, within GFF1240 at 72.451 kb on - strand at 72.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 70,346 - GFF1237 0.66 +1.1 70,346 - GFF1237 0.66 +1.0 70,367 - +0.6 70,424 + +0.6 70,432 - +1.4 70,469 + GFF1238 0.14 +0.2 70,505 - GFF1238 0.18 -0.9 70,597 + GFF1238 0.30 +0.3 70,597 + GFF1238 0.30 +0.7 70,598 - GFF1238 0.30 +1.0 70,598 - GFF1238 0.30 -0.4 70,601 + GFF1238 0.31 +1.2 70,601 + GFF1238 0.31 -0.7 70,601 + GFF1238 0.31 +0.1 70,601 + GFF1238 0.31 -1.6 70,601 + GFF1238 0.31 -1.0 70,602 - GFF1238 0.31 +1.1 70,602 - GFF1238 0.31 -0.8 70,602 - GFF1238 0.31 -0.7 70,602 - GFF1238 0.31 -0.0 70,664 + GFF1238 0.39 -2.1 70,665 - GFF1238 0.39 -0.9 70,665 - GFF1238 0.39 -0.1 70,701 + GFF1238 0.44 +1.4 70,729 + GFF1238 0.47 +1.1 70,927 + GFF1238 0.73 +2.1 70,928 - GFF1238 0.73 -0.1 70,989 - GFF1238 0.81 -4.6 70,989 - GFF1238 0.81 -4.3 71,132 + -0.4 71,318 + -0.8 71,412 + GFF1239 0.64 -1.4 71,461 + +1.2 71,476 - -0.9 71,479 + +0.0 71,536 - +0.5 71,557 + -0.2 71,558 - -0.5 71,558 - +0.6 71,683 + +0.2 71,686 - -5.0 71,729 + +0.6 71,740 - GFF1240 0.11 +0.6 71,819 - GFF1240 0.20 -2.3 71,872 - GFF1240 0.26 +0.7 71,895 + GFF1240 0.28 -0.3 71,899 - GFF1240 0.29 +0.4 71,902 + GFF1240 0.29 -0.6 72,451 - +0.3 72,458 - +0.0
Or see this region's nucleotide sequence