Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF113

Experiment: Ffm_phage 10 MOI; agar plate assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF112 and GFF113 are separated by 234 nucleotidesGFF113 and GFF114 are separated by 245 nucleotides GFF112 - 2-keto-3-deoxygluconate permease (KDG permease), at 97,937 to 98,890 GFF112 GFF113 - FIG01045153: hypothetical protein, at 99,125 to 99,487 GFF113 GFF114 - HNH endonuclease, at 99,733 to 100,575 GFF114 Position (kb) 99 100Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 98.335 kb on + strand, within GFF112at 98.335 kb on + strand, within GFF112at 98.335 kb on + strand, within GFF112at 98.465 kb on - strand, within GFF112at 98.477 kb on - strand, within GFF112at 98.477 kb on - strand, within GFF112at 98.530 kb on - strand, within GFF112at 98.650 kb on + strand, within GFF112at 98.651 kb on - strand, within GFF112at 98.651 kb on - strand, within GFF112at 98.777 kb on - strand, within GFF112at 99.213 kb on + strand, within GFF113at 99.300 kb on + strand, within GFF113at 99.391 kb on + strand, within GFF113at 99.533 kb on + strandat 99.534 kb on - strandat 99.540 kb on - strandat 99.544 kb on - strandat 99.553 kb on - strandat 99.553 kb on - strandat 99.553 kb on - strandat 99.693 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay
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98,335 + GFF112 0.42 -2.2
98,335 + GFF112 0.42 -1.2
98,335 + GFF112 0.42 +0.0
98,465 - GFF112 0.55 +0.2
98,477 - GFF112 0.57 -0.8
98,477 - GFF112 0.57 -0.2
98,530 - GFF112 0.62 -1.9
98,650 + GFF112 0.75 +1.3
98,651 - GFF112 0.75 -0.0
98,651 - GFF112 0.75 -0.7
98,777 - GFF112 0.88 -0.1
99,213 + GFF113 0.24 -4.2
99,300 + GFF113 0.48 -1.0
99,391 + GFF113 0.73 -2.5
99,533 + +0.7
99,534 - +0.5
99,540 - -3.0
99,544 - +0.5
99,553 - -2.0
99,553 - -1.9
99,553 - +0.1
99,693 - +0.0

Or see this region's nucleotide sequence