Experiment: Ffm_phage 10 MOI; agar plate assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1082 and GFF1083 are separated by 241 nucleotides GFF1083 and GFF1084 are separated by 110 nucleotides GFF1084 and GFF1085 are separated by 56 nucleotides
GFF1082 - Putative alanine/glycine transport protein, at 266,750 to 268,207
GFF1082
GFF1083 - Transaldolase (EC 2.2.1.2), at 268,449 to 269,402
GFF1083
GFF1084 - Molybdopterin biosynthesis molybdochelatase MogA, at 269,513 to 270,103
GFF1084
GFF1085 - YaaH protein, at 270,160 to 270,726
GFF1085
Position (kb) 268
269
270 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 267.522 kb on - strand, within GFF1082 at 267.543 kb on - strand, within GFF1082 at 267.608 kb on + strand, within GFF1082 at 267.784 kb on + strand, within GFF1082 at 267.785 kb on - strand, within GFF1082 at 267.787 kb on - strand, within GFF1082 at 267.787 kb on - strand, within GFF1082 at 267.832 kb on + strand, within GFF1082 at 267.985 kb on + strand, within GFF1082 at 268.149 kb on - strand at 268.223 kb on + strand at 268.243 kb on + strand at 268.243 kb on + strand at 268.261 kb on + strand at 268.262 kb on - strand at 268.287 kb on + strand at 268.287 kb on + strand at 268.287 kb on + strand at 268.302 kb on + strand at 268.328 kb on - strand at 268.328 kb on - strand at 268.392 kb on - strand at 268.528 kb on - strand at 268.544 kb on + strand at 268.545 kb on - strand, within GFF1083 at 268.545 kb on - strand, within GFF1083 at 268.595 kb on + strand, within GFF1083 at 268.731 kb on - strand, within GFF1083 at 268.796 kb on + strand, within GFF1083 at 268.907 kb on + strand, within GFF1083 at 268.984 kb on + strand, within GFF1083 at 268.985 kb on - strand, within GFF1083 at 268.991 kb on + strand, within GFF1083 at 269.348 kb on + strand at 269.400 kb on + strand at 269.423 kb on + strand at 269.435 kb on + strand at 269.436 kb on - strand at 269.518 kb on + strand at 269.518 kb on + strand at 269.519 kb on - strand at 269.523 kb on + strand at 269.523 kb on + strand at 269.523 kb on + strand at 269.524 kb on - strand at 269.524 kb on - strand at 269.527 kb on + strand at 269.649 kb on + strand, within GFF1084 at 269.950 kb on - strand, within GFF1084 at 270.009 kb on + strand, within GFF1084 at 270.010 kb on - strand, within GFF1084 at 270.030 kb on - strand, within GFF1084 at 270.119 kb on - strand at 270.150 kb on + strand at 270.231 kb on - strand, within GFF1085 at 270.231 kb on - strand, within GFF1085 at 270.280 kb on - strand, within GFF1085 at 270.280 kb on - strand, within GFF1085 at 270.301 kb on - strand, within GFF1085 at 270.390 kb on + strand, within GFF1085 at 270.391 kb on - strand, within GFF1085
Per-strain Table
Position Strand Gene LocusTag Fraction Ffm_phage 10 MOI; agar plate assay remove 267,522 - GFF1082 0.53 +0.6 267,543 - GFF1082 0.54 -2.1 267,608 + GFF1082 0.59 -0.2 267,784 + GFF1082 0.71 -0.4 267,785 - GFF1082 0.71 +0.2 267,787 - GFF1082 0.71 -0.1 267,787 - GFF1082 0.71 +0.3 267,832 + GFF1082 0.74 +0.7 267,985 + GFF1082 0.85 -0.5 268,149 - -0.9 268,223 + +0.2 268,243 + +0.4 268,243 + -2.0 268,261 + +0.5 268,262 - -0.5 268,287 + +0.4 268,287 + +1.0 268,287 + +2.6 268,302 + +1.4 268,328 - +0.6 268,328 - +0.2 268,392 - +1.0 268,528 - -0.4 268,544 + +1.3 268,545 - GFF1083 0.10 -1.5 268,545 - GFF1083 0.10 -1.0 268,595 + GFF1083 0.15 +0.4 268,731 - GFF1083 0.30 -0.0 268,796 + GFF1083 0.36 -0.1 268,907 + GFF1083 0.48 -2.7 268,984 + GFF1083 0.56 +1.2 268,985 - GFF1083 0.56 -1.3 268,991 + GFF1083 0.57 +1.8 269,348 + +0.2 269,400 + -1.5 269,423 + +0.3 269,435 + +0.5 269,436 - -0.5 269,518 + -2.7 269,518 + +2.8 269,519 - -2.8 269,523 + -1.9 269,523 + -4.5 269,523 + -1.6 269,524 - +1.5 269,524 - +0.1 269,527 + -0.3 269,649 + GFF1084 0.23 -0.2 269,950 - GFF1084 0.74 +0.1 270,009 + GFF1084 0.84 -1.1 270,010 - GFF1084 0.84 -0.8 270,030 - GFF1084 0.87 -1.3 270,119 - -1.3 270,150 + -0.9 270,231 - GFF1085 0.13 +0.7 270,231 - GFF1085 0.13 +0.4 270,280 - GFF1085 0.21 +0.5 270,280 - GFF1085 0.21 +0.2 270,301 - GFF1085 0.25 +0.2 270,390 + GFF1085 0.41 +0.6 270,391 - GFF1085 0.41 -0.3
Or see this region's nucleotide sequence