Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3478 and Atu3479 are separated by 7 nucleotides Atu3479 and Atu3480 are separated by 1 nucleotides Atu3480 and Atu3481 overlap by 11 nucleotides
Atu3478: Atu3478 - 3-methylcrotonoyl-CoA carboxylase beta subunit, at 525,705 to 527,306
Atu3478
Atu3479: Atu3479 - 3-methylcrotonyl-CoA carboxylase subunit alpha, at 527,314 to 529,305
Atu3479
Atu3480: Atu3480 - hydroxymethylglutaryl-CoA lyase, at 529,307 to 530,233
Atu3480
Atu3481: Atu3481 - enoyl-CoA hydratase, at 530,223 to 530,999
Atu3481
Position (kb) 527
528
529
530 Strain fitness (log2 ratio) -2
-1
0
1 at 526.352 kb on + strand, within Atu3478 at 526.379 kb on - strand, within Atu3478 at 526.389 kb on + strand, within Atu3478 at 526.474 kb on - strand, within Atu3478 at 526.509 kb on - strand, within Atu3478 at 526.565 kb on + strand, within Atu3478 at 526.570 kb on + strand, within Atu3478 at 526.622 kb on - strand, within Atu3478 at 526.632 kb on - strand, within Atu3478 at 526.682 kb on - strand, within Atu3478 at 526.890 kb on + strand, within Atu3478 at 526.979 kb on + strand, within Atu3478 at 526.979 kb on + strand, within Atu3478 at 526.979 kb on + strand, within Atu3478 at 527.045 kb on + strand, within Atu3478 at 527.104 kb on + strand, within Atu3478 at 527.232 kb on + strand at 527.240 kb on - strand at 527.265 kb on - strand at 527.389 kb on - strand at 527.439 kb on + strand at 527.443 kb on - strand at 527.534 kb on + strand, within Atu3479 at 527.614 kb on + strand, within Atu3479 at 527.796 kb on - strand, within Atu3479 at 528.004 kb on + strand, within Atu3479 at 528.052 kb on - strand, within Atu3479 at 528.083 kb on - strand, within Atu3479 at 528.131 kb on + strand, within Atu3479 at 528.192 kb on + strand, within Atu3479 at 528.273 kb on + strand, within Atu3479 at 528.287 kb on - strand, within Atu3479 at 528.490 kb on + strand, within Atu3479 at 528.707 kb on + strand, within Atu3479 at 528.840 kb on - strand, within Atu3479 at 528.840 kb on - strand, within Atu3479 at 528.859 kb on - strand, within Atu3479 at 528.871 kb on + strand, within Atu3479 at 528.949 kb on - strand, within Atu3479 at 528.970 kb on + strand, within Atu3479 at 528.976 kb on + strand, within Atu3479 at 529.088 kb on + strand, within Atu3479 at 529.268 kb on + strand at 529.276 kb on - strand at 529.447 kb on - strand, within Atu3480 at 529.447 kb on - strand, within Atu3480 at 529.518 kb on - strand, within Atu3480 at 529.530 kb on - strand, within Atu3480 at 529.672 kb on - strand, within Atu3480 at 529.693 kb on + strand, within Atu3480 at 529.816 kb on - strand, within Atu3480 at 529.877 kb on + strand, within Atu3480 at 529.938 kb on + strand, within Atu3480 at 529.938 kb on + strand, within Atu3480 at 529.982 kb on + strand, within Atu3480 at 530.036 kb on - strand, within Atu3480 at 530.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 526,352 + Atu3478 0.40 -0.5 526,379 - Atu3478 0.42 -0.4 526,389 + Atu3478 0.43 -0.8 526,474 - Atu3478 0.48 -0.2 526,509 - Atu3478 0.50 +1.2 526,565 + Atu3478 0.54 -1.4 526,570 + Atu3478 0.54 -1.6 526,622 - Atu3478 0.57 +0.2 526,632 - Atu3478 0.58 -1.1 526,682 - Atu3478 0.61 -0.9 526,890 + Atu3478 0.74 +0.2 526,979 + Atu3478 0.80 +0.0 526,979 + Atu3478 0.80 -1.2 526,979 + Atu3478 0.80 +1.2 527,045 + Atu3478 0.84 -1.3 527,104 + Atu3478 0.87 +0.8 527,232 + -1.1 527,240 - -0.5 527,265 - -0.9 527,389 - -0.6 527,439 + -0.3 527,443 - -1.1 527,534 + Atu3479 0.11 -1.8 527,614 + Atu3479 0.15 -1.3 527,796 - Atu3479 0.24 -1.6 528,004 + Atu3479 0.35 -1.7 528,052 - Atu3479 0.37 -0.7 528,083 - Atu3479 0.39 +0.2 528,131 + Atu3479 0.41 +0.2 528,192 + Atu3479 0.44 -0.5 528,273 + Atu3479 0.48 -0.8 528,287 - Atu3479 0.49 -0.4 528,490 + Atu3479 0.59 -0.9 528,707 + Atu3479 0.70 +0.6 528,840 - Atu3479 0.77 -0.5 528,840 - Atu3479 0.77 -1.5 528,859 - Atu3479 0.78 -1.4 528,871 + Atu3479 0.78 -0.6 528,949 - Atu3479 0.82 -0.6 528,970 + Atu3479 0.83 -1.1 528,976 + Atu3479 0.83 -0.4 529,088 + Atu3479 0.89 -0.4 529,268 + -0.6 529,276 - -1.1 529,447 - Atu3480 0.15 -1.0 529,447 - Atu3480 0.15 -1.2 529,518 - Atu3480 0.23 -1.1 529,530 - Atu3480 0.24 +0.5 529,672 - Atu3480 0.39 -0.6 529,693 + Atu3480 0.42 -0.6 529,816 - Atu3480 0.55 -0.5 529,877 + Atu3480 0.61 -0.2 529,938 + Atu3480 0.68 -1.5 529,938 + Atu3480 0.68 -2.3 529,982 + Atu3480 0.73 -0.9 530,036 - Atu3480 0.79 -0.8 530,206 + -0.1
Or see this region's nucleotide sequence