Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3323 and Atu3324 are separated by 83 nucleotides Atu3324 and Atu3325 overlap by 4 nucleotides
Atu3323: Atu3323 - HlyD family secretion protein, at 356,437 to 357,795
Atu3323
Atu3324: Atu3324 - hypothetical protein, at 357,879 to 358,373
Atu3324
Atu3325: Atu3325 - exopolysaccharide production protein, at 358,370 to 359,638
Atu3325
Position (kb)
357
358
359 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 356.976 kb on + strand, within Atu3323 at 357.106 kb on + strand, within Atu3323 at 357.155 kb on - strand, within Atu3323 at 357.322 kb on + strand, within Atu3323 at 357.374 kb on + strand, within Atu3323 at 357.380 kb on + strand, within Atu3323 at 357.498 kb on + strand, within Atu3323 at 357.613 kb on - strand, within Atu3323 at 357.620 kb on - strand, within Atu3323 at 357.621 kb on + strand, within Atu3323 at 357.631 kb on - strand, within Atu3323 at 357.719 kb on - strand at 357.808 kb on + strand at 357.816 kb on - strand at 357.850 kb on + strand at 357.897 kb on - strand at 357.932 kb on + strand, within Atu3324 at 358.075 kb on - strand, within Atu3324 at 358.096 kb on - strand, within Atu3324 at 358.200 kb on + strand, within Atu3324 at 358.220 kb on + strand, within Atu3324 at 358.263 kb on - strand, within Atu3324 at 358.321 kb on - strand, within Atu3324 at 358.421 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 356,976 + Atu3323 0.40 -1.4 357,106 + Atu3323 0.49 -0.9 357,155 - Atu3323 0.53 -0.1 357,322 + Atu3323 0.65 -0.4 357,374 + Atu3323 0.69 +0.2 357,380 + Atu3323 0.69 -0.6 357,498 + Atu3323 0.78 +0.2 357,613 - Atu3323 0.87 -1.8 357,620 - Atu3323 0.87 -0.3 357,621 + Atu3323 0.87 -0.5 357,631 - Atu3323 0.88 -0.8 357,719 - -0.2 357,808 + +0.6 357,816 - +0.6 357,850 + -0.2 357,897 - -1.9 357,932 + Atu3324 0.11 -0.2 358,075 - Atu3324 0.40 -0.8 358,096 - Atu3324 0.44 +0.2 358,200 + Atu3324 0.65 -0.8 358,220 + Atu3324 0.69 +1.2 358,263 - Atu3324 0.78 -3.4 358,321 - Atu3324 0.89 -2.1 358,421 - +0.5
Or see this region's nucleotide sequence